Biomedical Applications of Bacterial Inclusion Bodies

Ratera, Imma; Peternel, Špela; Seras‐Franzoso, Joaquin; Cano‐Garrido, Olivia; García‐Fruitós, Elena; Cubarsi, Rafael; Vázquez, Esther; Corchero, José Luís; Rodríguez‐Carmona, Escarlata; Veciana, Jaume; Villaverde, Antonio

doi:10.1002/9781118845363.ch8

Cited by 4 publications

(3 citation statements)

References 81 publications

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“…Last but not least, we will briefly outline the application potential for CatIBs for biocatalysis, synthetic chemistry, and biotechnology. Here, we will not focus on biomedical applications of CatIBs or IBs as this aspect has been reviewed recently (Ratera et al 2014 ; Krauss et al 2017 ).…”

Section: Biotechnological Potential and Application Of Catibsmentioning

confidence: 99%

“…The resulting CatIBs can thus be considered as cellularly produced, insoluble bionanomaterials, or protein immobilizates (Fig. 1d ) with potential application in biocatalysis, synthetic chemistry, and biomedicine (Yang et al 2018 ; Jäger et al 2018 ; Jäger et al 2019b ; Kloss et al 2018a , b ; Diener et al 2016 ; Nahalka 2008 ; Nahalka and Nidetzky 2007 ; Nahalka and Patoprsty 2009 ; Nahalka et al 2008 ; Ratera et al 2014 ; Rueda et al 2014 ; García-Fruitós et al 2009 ; Vazquez et al 2012 ). Since CatIBs are produced heterologously in bacteria, it is not surprising that different parameters, like fusion protein design, expression conditions, and downstream processing, strongly influence not only the general success of immobilization as CatIBs but also their properties.…”

Section: Introductionmentioning

confidence: 99%

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Catalytically-active inclusion bodies for biotechnology—general concepts, optimization, and application

Jäger

Lamm

Küsters

et al. 2020

Appl Microbiol Biotechnol

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Bacterial inclusion bodies (IBs) have long been considered as inactive, unfolded waste material produced by heterologous overexpression of recombinant genes. In industrial applications, they are occasionally used as an alternative in cases where a protein cannot be expressed in soluble form and in high enough amounts. Then, however, refolding approaches are needed to transform inactive IBs into active soluble protein. While anecdotal reports about IBs themselves showing catalytic functionality/ activity (CatIB) are found throughout literature, only recently, the use of protein engineering methods has facilitated the ondemand production of CatIBs. CatIB formation is induced usually by fusing short peptide tags or aggregation-inducing protein domains to a target protein. The resulting proteinaceous particles formed by heterologous expression of the respective genes can be regarded as a biologically produced bionanomaterial or, if enzymes are used as target protein, carrier-free enzyme immobilizates. In the present contribution, we review general concepts important for CatIB production, processing, and application. Key points • Catalytically active inclusion bodies (CatIBs) are promising bionanomaterials. • Potential applications in biocatalysis, synthetic chemistry, and biotechnology. • CatIB formation represents a generic approach for enzyme immobilization. • CatIB formation efficiency depends on construct design and expression conditions.

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Section: Biotechnological Potential and Application Of Catibsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Catalytically-active inclusion bodies for biotechnology—general concepts, optimization, and application

Jäger

Lamm

Küsters

et al. 2020

Appl Microbiol Biotechnol

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“…Improving our knowledge on protein stability under concentrated conditions is of fundamental relevance in several research fields, such as cell biology or biomedical science, , and also has practical applications in various industrial sectors, including the manufacture of food products, biomaterials, and therapeutic proteins . In this study, we focus on the aggregation of monoclonal antibodies (mAbs), which have emerged as a major class of pharmaceuticals since the 1980s .…”

Section: Introductionmentioning

confidence: 99%

Kinetics of Monoclonal Antibody Aggregation from Dilute toward Concentrated Conditions

et al. 2016

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Gaining understanding on the aggregation behavior of proteins under concentrated conditions is of both fundamental and industrial relevance. Here, we study the aggregation kinetics of a model monoclonal antibody (mAb) under thermal stress over a wide range of protein concentrations in various buffer solutions. We follow experimentally the monomer depletion and the aggregate growth by size exclusion chromatography with inline light scattering. We describe the experimental results in the frame of a kinetic model based on population balance equations, which allows one to discriminate the contributions of the conformational and of the colloidal stabilities to the global aggregation rate. Finally, we propose an expression for the aggregation rate constant, which accounts for solution viscosity, protein-protein interactions, as well as aggregate compactness. All these effects can be quantified by light scattering techniques. It is found that the model describes well the experimental data under dilute conditions. Under concentrated conditions, good model predictions are obtained when the solution pH is far below the isoelectric point (pI) of the mAb. However, peculiar effects arise when the solution pH is increased toward the mAb pI, and possible explanations are discussed.

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High cell density cultivation of E. coli in shake flasks for the production of recombinant proteins

Ganjave

Dodia

Sunder

et al. 2022

Biotechnology Reports

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Highlights True fed-batch strategy for high cell density cultivation of E. coli in shake flask. Cybernetic model-based optimization of the feeding recipe. Biomass of 19.9–21.5 g DCW/L, in agreement with the model prediction. Volumetric productivity for tested proteins increased 8–34-fold compared to batch. Scale up of fed-batch recipe to bioreactor resulted in further 2.8-fold increase.

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Biomedical Applications of Bacterial Inclusion Bodies

Cited by 4 publications

References 81 publications

Catalytically-active inclusion bodies for biotechnology—general concepts, optimization, and application

Catalytically-active inclusion bodies for biotechnology—general concepts, optimization, and application

Kinetics of Monoclonal Antibody Aggregation from Dilute toward Concentrated Conditions

High cell density cultivation of E. coli in shake flasks for the production of recombinant proteins

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