Biomedical applications of engineered heparin-based materials

Nazarzadeh Zare, Ehsan; Khorsandi, Danial; Zarepour, Atefeh; Yilmaz, Hulya; Agarwal, Tarun; Hooshmand, Sara; Mohammadinejad, Reza; Ozdemir, Fatma; Sahin, Onur; Adiguzel, Sevin; Khan, Haroon; Zarrabi, Ali; Sharifi, Esmaeel; Kumar, Arun; Mostafavi, Ebrahim; Kouchehbaghi, Negar Hosseinzadeh; Mattoli, Virgilio; Zhang, Feng; Jucaud, Vadim; Najafabadi, Alireza Hassani; Khademhosseini, Ali

doi:10.1016/j.bioactmat.2023.08.002

Cited by 13 publications

(10 citation statements)

References 263 publications

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“…As the number of LBL layers increases, so does the number of Hep grafts. Hep improves the catheter’s surface hydrophilicity and negative charge to enhance the antibacterial adhesion and coagulation of the SR catheters. , …”

Section: Resultsmentioning

confidence: 99%

“…Hep improves the catheter's surface hydrophilicity and negative charge to enhance the antibacterial adhesion and coagulation of the SR catheters. 19,46 Furthermore, the coating's stability plays a crucial role in its application. 10,21 We evaluated this aspect by immersing the catheters in PBS for 15 days, simulating the physiological environment (Figure S3a).…”

Section: Surface Characterizationmentioning

confidence: 99%

“…In recent years, there have been significant advancements in the development of advanced medical catheters. − Catheter coating is an effective way to treat bacterial infections and thrombosis caused by catheter implantation. , However, current antibacterial coatings, including those involving chemical modification, silver ions, and inorganic nanoparticles, still exhibit some limitations. For instance, the chemical modification method is limited by substrate compatibility, and the use of silver ion antibacterial agents poses certain biosafety risks. , Hep has been modified on the surface of the coating and remains the most effective means of anticoagulation .…”

Section: Introductionmentioning

confidence: 99%

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Generalized Multifunctional Coating Strategies Based on Polyphenol-Amine-Inspired Chemistry and Layer-by-Layer Deposition for Blood Contact Catheters

Du,

Zhang,

et al. 2024

ACS Biomater. Sci. Eng.

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Blood-contacting catheters play a pivotal role in contemporary medical treatments, particularly in the management of cardiovascular diseases. However, these catheters exhibit inappropriate wettability and lack antimicrobial characteristics, which often lead to catheter-related infections and thrombosis. Therefore, there is an urgent need for blood contact catheters with antimicrobial and anticoagulant properties. In this study, we employed tannic acid (TA) and 3-aminopropyltriethoxysilane (APTES) to create a stable hydrophilic coating under mild conditions. Heparin (Hep) and poly(lysine) (PL) were then modified on the TA-APTES coating surface using the layer-by-layer (LBL) technique to create a superhydrophilic TA/APTES/ (LBL) 4 coating on silicone rubber (SR) catheters. Leveraging the superhydrophilic nature of this coating, it can be effectively applied to blood-contacting catheters to impart antibacterial, antiprotein adsorption, and anticoagulant properties. Due to Hep's anticoagulant attributes, the activated partial thromboplastin time and thrombin time tests conducted on SR/TA-APTES/(LBL) 4 catheters revealed remarkable extensions of 276 and 103%, respectively, when compared to uncoated commercial SR catheters. Furthermore, the synergistic interaction between PL and TA serves to enhance the resistance of SR/TA-APTES/(LBL) 4 catheters against bacterial adherence, reducing it by up to 99.9% compared to uncoated commercial SR catheters. Remarkably, the SR/TA-APTES/(LBL) 4 catheter exhibits good biocompatibility with human umbilical vein endothelial cells in culture, positioning it as a promising solution to address the current challenges associated with blood-contact catheters.

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Section: Resultsmentioning

confidence: 99%

Section: Surface Characterizationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Generalized Multifunctional Coating Strategies Based on Polyphenol-Amine-Inspired Chemistry and Layer-by-Layer Deposition for Blood Contact Catheters

Du,

Zhang,

et al. 2024

ACS Biomater. Sci. Eng.

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“…Heparin, a component of the extracellular matrix, exhibits a notable affinity toward diverse GFs, which may be a suitable carrier for the sequestration and controlled release of GFs in DMEP via chemical conjugation or electrostatic interactions . However, heparin lacks the ability to induce odontogenic differentiation, which is indispensable for the regeneration of pulp-dentin complex.…”

Section: Introductionmentioning

confidence: 99%

Heparin-Functionalized Bioactive Glass to Harvest Endogenous Growth Factors for Pulp Regeneration

Wu,

Li,

Mao

et al. 2024

ACS Appl. Mater. Interfaces

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Pulp and periapical diseases can lead to the cessation of tooth development, resulting in compromised tooth structure and functions. Despite numerous efforts to induce pulp regeneration, effective strategies are still lacking. Growth factors (GFs) hold considerable promise in pulp regeneration due to their diverse cellular regulatory properties. However, the limited half-lives and susceptibility to degradation of exogenous GFs necessitate the administration of supra-physiological doses, leading to undesirable side effects. In this research, a heparin-functionalized bioactive glass (CaO−P 2 O 5 −SiO 2 −Heparin, abbreviated as PSC-Heparin) with strong bioactivity and a stable neutral pH is developed as a promising candidate to addressing challenges in pulp regeneration. Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and thermogravimetric analysis reveal the successful synthesis of PSC-Heparin. Scanning electron microscopy and X-ray diffraction show the hydroxyapatite formation can be observed on the surface of PSC-Heparin after soaking in simulated body fluid for 12 h. PSC-Heparin is capable of harvesting various endogenous GFs and sustainably releasing them over an extended duration by the enzyme-linked immunosorbent assay. Cytological experiments show that developed PSC-Heparin can facilitate the adhesion, migration, proliferation, and odontogenic differentiation of stem cells from apical papillae. Notably, the histological analysis of subcutaneous implantation in nude mice demonstrates PSC-Heparin is capable of promoting the odontoblast-like layers and pulp-dentin complex formation without the addition of exogenous GFs, which is vital for clinical applications. This work highlights an effective strategy of harvesting endogenous GFs and avoiding the involvement of exogenous GFs to achieve pulp-dentin complex regeneration, which may open a new horizon for regenerative endodontic therapy.

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“…The capacity of polyelectrolytes to interact with proteins was exploited in the development of some systems used in the separation or purification of proteins [ 18 , 19 , 20 ] or some support materials for protein release [ 21 , 22 , 23 ]. The interaction between protein and polyelectrolyte can form soluble complexes, precipitates (insoluble complexes), or complex coacervates as a function of the medium condition (ionic strength, pH, protein/polyelectrolyte ratio) [ 24 , 25 ].…”

Section: Introductionmentioning

confidence: 99%

Effect of pH on the Poly(acrylic acid)/Poly(vinyl alcohol)/Lysozyme Complexes Formation

Morariu,

Avadanei,

Nita

2023

Molecules

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The interactions between poly(acrylic acid) (PAA), poly(vinyl alcohol) (PVA), and lysozyme (Lys) in an aqueous environment at pHs of 2, 4, and 7.4 were discussed considering the experimental data obtained by turbidimetry, electrokinetic and rheological measurements, and FTIR analysis. It was found that the increase in PAA amount reduces the coacervation zone by shifting the critical pHcr1to higher values while the critical pHcr2 remains unchanged. The coacervation zone extended from 3.1–4.2 to 2.9–4.7 increasing the Lys concentration from 0.2% to 0.5%. The zeta potential measurements showed that the PAA–PVA–Lys mixture in water is the most stable in the pH range of 4.5–8. Zero shear viscosity exhibited deviations from additivity at both investigated pHs, and a maximum value corresponding to a maximum hydrodynamic volume was revealed at PAA weight fractions of 0.4 and 0.5 for pHs of 4 and 7.4, respectively. The binding affinity to Lys of PAA, established by molecular dynamics simulation, was slightly higher than that of PVA. The more stable complex was PAA–Lys formed in a very acidic environment; for that, a binding affinity of −7.1 kcal/mol was determined.

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Biomedical applications of engineered heparin-based materials

Cited by 13 publications

References 263 publications

Generalized Multifunctional Coating Strategies Based on Polyphenol-Amine-Inspired Chemistry and Layer-by-Layer Deposition for Blood Contact Catheters

Generalized Multifunctional Coating Strategies Based on Polyphenol-Amine-Inspired Chemistry and Layer-by-Layer Deposition for Blood Contact Catheters

Heparin-Functionalized Bioactive Glass to Harvest Endogenous Growth Factors for Pulp Regeneration

Effect of pH on the Poly(acrylic acid)/Poly(vinyl alcohol)/Lysozyme Complexes Formation

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