Biomedical applications of FTIR difference spectroscopy

Moss, David A.; Fuechsle, Kathrin; Masuch, Ralf; Wolf, A.

doi:10.1117/12.384962

Cited by 4 publications

(4 citation statements)

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“…In litter FTIR spectra, the two phosphodiester bands of DNA could be resolved through FTIR difference spectroscopy. FTIR difference spectroscopy is a method to overcome the problem that small changes in the molecular structure of a complex tissue are difficult to study as the corresponding spectral changes are very small compared to the signals of the unchanged residual organic matter (Moss et al, 2000). Yet, a subtraction of two FTIR spectra (unmodified and modified tissue) leads to a FTIR difference spectrum that selectively shows spectral changes.…”

Section: Quantification Of Microbial N In Litter From Ftirmentioning

confidence: 99%

“…On the other hand, stoichiometric considerations predict lower amounts of microbial biomass and thus microbial N for litters with lower C loss as the total decomposition process is less advanced. In order to place the microbial N content of different samples into a relationship with one another and to take variations in C loss into account, we assumed microbial homeostasis and a microbial C/N ratio of 5 (Mouginot et al, 2014). The thereby determinable total amount of newly formed microbial C was set in relation to the litter C loss, which leads to an estimate of the CUE (Manzoni, 2017).…”

Section: Microbial N In Decomposed Littermentioning

confidence: 99%

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Evidence for preferential protein depolymerization in wetland soils in response to external nitrogen availability provided by a novel FTIR routine

et al. 2020

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Abstract. Phragmites australis litters were incubated in three waterlogged anoxic wetland soils of different nutrient status for 75 d, and litter nitrogen (N) dynamics were analyzed by elemental analyses and Fourier transform infrared spectroscopy (FTIR). At the end of the incubation time, the N content in the remaining litter tissue had increased in most samples. Yet, the increase in N content was less pronounced when litters had been decomposed in a more-N-poor environment. FTIR was used to quantify the relative content of proteins in litter tissue and revealed a highly linear relationship between bulk N content and protein content. Changes in bulk N content thus paralleled and probably were governed by changes in litter protein content. Such changes are the result of two competing processes within decomposing litter: enzymatic protein depolymerization as a part of the litter breakdown process and microbial protein synthesis as a part of microbial biomass growth within the litter. Assuming microbial homeostasis, DNA signals in FTIR spectra were used to calculate the amount of microbial N in decomposed litter which ranged from 14 % to 42 % of the total litter N for all leaf samples. Microbial carbon (C) content and resultant calculated carbon use efficiencies (CUEs) indicate that microbial N in litter accumulated according to predictions of the stoichiometric decomposition theory. Subtracting microbial C and N contributions from litter, however, revealed site-dependent variations in the percentual amount of the remaining still-unprocessed plant N in litter compared to remaining plant C, an indicator for preferential protein depolymerization. For all leaf litters, the coefficient of preferential protein depolymerization (α), which relates N-compound depolymerization to C-compound depolymerization, ranged from 0.74–0.88 in a nutrient-rich detritus mud to 1.38–1.82 in Sphagnum peat, the most nutrient-poor substrate in this experiment. Preferential protein depolymerization from litter decomposing in Sphagnum peat leads to a gradual N depletion in the early phase of litter decomposition, which we propose as a preservation mechanism for vascular litter in Sphagnum peatlands.

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Section: Quantification Of Microbial N In Litter From Ftirmentioning

confidence: 99%

Section: Microbial N In Decomposed Littermentioning

confidence: 99%

Evidence for preferential protein depolymerization in wetland soils in response to external nitrogen availability provided by a novel FTIR routine

et al. 2020

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“…Amide I line arises from the C=O hydrogen bonded stretching vibrations and Amide II line arises from the C-N stretching and CNH bending vibrations Other bands at 1240 cm -1 and 1076 cm-1 are due to PO-2 ionized asymmetric and symmetric stretching of RNA and DNA spectra [3]. A useful technique was introduced in the 1980s by several groups is the FTIR difference spectroscopy which could show very slight changes in cells [4].…”

Section: Introductionmentioning

confidence: 99%

New Applications of Lasers in Photobiology and Photochemistry

Badr

Kareim

2005

AIP Conference Proceedings

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“…Amide I line arises from the C=O hydrogen bonded stretching vibrations and Amide II line arises from the C-N stretching and CNH bending vibrations Other bands at 1240 cm' and 1076 cm1 are due to P0 2 ionized asymmetric and symmetric stretching of RNA and DNA spectra [3]. show very slight changes in cells [4]. he most widely employed technique for detecting non-palpable highly curable breast cancer.…”

Section: -Introductionmentioning

confidence: 99%