Biomedical Applications of Microfluidic Devices: A Review

Gharıb, Ghazaleh; Bütün, İsmail; Muganlı, Zülâl; Kozalak, Gül; Namlı, İlayda; Sarraf, Seyedali Seyedmirzaei; Ahmadi, Vahid; Toyran, Erçil; Wijnen, André J. van; Koşar, Ali

doi:10.3390/bios12111023

Cited by 78 publications

(26 citation statements)

References 485 publications

(595 reference statements)

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“…As transparency permits, oxygen, carbon dioxide, or other chemical compounds could be detected using optical methods. While it is challenging to use biocompatible materials, the difficulties in their use have been overcome by developing various fabrication techniques such as moulding, 3D printing, nanofabrication, and etching [ 154 ]. Microfluidic cell culturing chambers developed with soft lithography, which is a subbranch of moulding techniques, have been used in haematological cancer studies [ 155 ], drug delivery [ 156 ], and treatment of bortezomib-resistant MM [ 157 ].…”

Section: Emerging Technologiesmentioning

confidence: 99%

Review on Bortezomib Resistance in Multiple Myeloma and Potential Role of Emerging Technologies

et al. 2023

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Multiple myeloma is a hematological cancer type. For its treatment, Bortezomib has been widely used. However, drug resistance to this effective chemotherapeutic has been developed for various reasons. 2D cell cultures and animal models have failed to understand the MM disease and Bortezomib resistance. It is therefore essential to utilize new technologies to reveal a complete molecular profile of the disease. In this review, we in-depth examined the possible molecular mechanisms that cause Bortezomib resistance and specifically addressed MM and Bortezomib resistance. Moreover, we also included the use of nanoparticles, 3D culture methods, microfluidics, and organ-on-chip devices in multiple myeloma. We also discussed whether the emerging technology offers the necessary tools to understand and prevent Bortezomib resistance in multiple myeloma. Despite the ongoing research activities on MM, the related studies cannot provide a complete summary of MM. Nanoparticle and 3D culturing have been frequently used to understand MM disease and Bortezomib resistance. However, the number of microfluidic devices for this application is insufficient. By combining siRNA/miRNA technologies with microfluidic devices, a complete molecular genetic profile of MM disease could be revealed. Microfluidic chips should be used clinically in personal therapy and point-of-care applications. At least with Bortezomib microneedles, it could be ensured that MM patients can go through the treatment process more painlessly. This way, MM can be switched to the curable cancer type list, and Bortezomib can be targeted for its treatment with fewer side effects.

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Section: Emerging Technologiesmentioning

confidence: 99%

Review on Bortezomib Resistance in Multiple Myeloma and Potential Role of Emerging Technologies

et al. 2023

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“…Microfluidic devices rely inherently on confined flow dynamics for a wide range of applications, including disease diagnostics (Gharib et al 2022), single-cell encapsulation and analysis (Ling et al 2020), droplet-based microreactors (Liu, Xiang & Ni 2020), and the fabrication of tailored particulate matter (Li et al 2018). Often, shallow rectangular channels are utilized in which suspended objects flow at roughly the same depth, and hence the same optical focus, thus allowing microscopy-based characterization and monitoring.…”

Section: Introductionmentioning

confidence: 99%

Order–disorder transitions within deformable particle suspensions in planar Poiseuille flow

Millett

2024

J. Fluid Mech.

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Three-dimensional simulations of the ordering of elastic capsule suspensions within planar Poiseuille flow channels are reported. The simulations utilize the immersed boundary method coupled with the lattice Boltzmann method to capture the complex flow-induced capsule deformations and hydrodynamic interactions within the suspensions. A parametric study is presented whereby the confinement ratio and the particle deformability are varied independently within a two-dimensional range relevant to this ordering phenomenon. The initial distribution of capsules is random, and the simulations evolve the system from a disordered state to an ordered one, while an order parameter that quantifies the fraction of capsules belonging to one-dimensional train assemblies is computed throughout time. A monotonic increase in ordering is observed with increasing deformability. However, an optimal confinement ratio is identified corresponding to a peak in the order parameter. This peak is attributed to the competition between increasing long-range capsule attractions and decreasing in-plane capsule density (with fixed volume fraction) as the confinement ratio increases. Simulations are also performed to understand how dispersity in capsule size and deformability impact the degree of ordering. It is shown that ordering is quite sensitive to dispersity in capsule size, and much less sensitive to dispersity in deformability. Overall, the results provide important insights for the design of microfluidic devices.

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“…There are numerous strategies to design and fabricate miniaturized biosensors. One of them is microfluidics, which has been widely explored [ 3 , 19 , 20 , 21 , 22 ]. Integrating a biosensor into a microfluidic chip brings miniaturization with extremely low sample consumption and continuous measurement of the analyte.…”

Section: Introductionmentioning

confidence: 99%

Microfluidic Electrochemical Glucose Biosensor with In Situ Enzyme Immobilization

et al. 2023

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The development and characterization of a microfluidic electrochemical glucose biosensor are presented herein. The transducer part is based on thin-film metal electrodes on a glass substrate. The biological recognition element of the biosensor is the pyrroloquinoline quinone–glucose dehydrogenase (PQQ-GdhB) enzyme, selectively in situ immobilized via microcontact printing of a mixed self-assembling monolayer (SAM) on a gold working electrode, while the microfluidic part of the device comprises microchannel and microfluidic connections formed in a polydimethylsiloxane (PDMS) elastomer. The electrode properties throughout all steps of biosensor construction and the biosensor response to glucose concentration and analyte flow rate were characterized by cyclic voltammetry and chronoamperometry. A measurement range of up to 10 mM in glucose concentration with a linear range up to 200 μM was determined. A detection limit of 30 µM in glucose concentration was obtained. Respective biosensor sensitivities of 0.79 nA/µM/mm2 and 0.61 nA/µM/mm2 were estimated with and without a flow at 20 µL/min. The developed approach of in situ enzyme immobilization can find a wide number of applications in the development of microfluidic biosensors, offering a path towards continuous and time-independent detection.

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Biomedical Applications of Microfluidic Devices: A Review

Cited by 78 publications

References 485 publications

Review on Bortezomib Resistance in Multiple Myeloma and Potential Role of Emerging Technologies

Review on Bortezomib Resistance in Multiple Myeloma and Potential Role of Emerging Technologies

Order–disorder transitions within deformable particle suspensions in planar Poiseuille flow

Microfluidic Electrochemical Glucose Biosensor with In Situ Enzyme Immobilization

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