Biomedical Applications of Raman Imaging

Spectroscopic Imaging of Deep Tissue through Photoacoustic Detection of Molecular Vibration

“…Vibrational spectroscopy and imaging based on infrared absorption, near infrared absorption, and spontaneous Raman scattering have been applied intensively to biomedicine 1–3 . With high sensitivity and 3-demensional sectioning capability, nonlinear vibrational microscopies based on coherent anti-Stokes Raman scattering 4,5 and stimulated Raman scattering 6,7 have shed new light on biomedical research 8 .…”

“…Vibrational spectroscopy and imaging based on infrared absorption, near-infrared absorption, and spontaneous Raman scattering have been applied intensively to biomedicine. − With high sensitivity and three-dimensional sectioning capability, nonlinear vibrational microscopies based on coherent anti-Stokes Raman scattering , and stimulated Raman scattering , have shed new light on biomedical research . While coherent Raman scattering microscopy offers submicrometer resolution for mapping subcellular structures, it employs ballistic photons under the tight focusing condition and thus has a limited tissue penetration depth of ∼100 μm, which prevents its potential use for imaging deep tissue in clinical settings.…”