The current research aims to elucidate the influence of reduction process of TiO2 nanostructures on the surface properties of a bioinspired Ti modified implant, considering that the interface between a biomaterial surface and the living tissue plays an important role for this interaction. The production of reduced TiO2 nanotubes (RNT) with lower band gap is optimized and their performance is compared with those of simple TiO2 nanotubes (NT). The more conductive surfaces provided by the presence of RNT on Ti, allow a facile deposition of silk fibroin (SF) film using the electrochemical deposition method. This hybrid film is then functionalized with ZnO nanoparticles, to improve the antibacterial effect of the coating. The modified Ti surface is evaluated in terms of surface chemistry, morphology and roughness, wettability, surface energy, surface charge and antibacterial properties. Surface analysis such as SEM, AFM, FTIR and contact angle measurements were performed to obtain topographical features and wettability.The antibacterial effect is studied for new created surfaces against Staphylococcus aureus and Escherichia coli. Changing the titanium surface morphology with RNT increases the bactericidal effect by 27%, then, after SF deposition, by 45% and for ZnO modified samples, the bactericidal rate increases up to 75%.