Biomedical Mass Spectrometry in Today's and Tomorrow's Clinical Microbiology Laboratories

Belkum, Alex van; Welker, Martin; Erhard, Marcel; Chatellier, Sonia

doi:10.1128/jcm.00420-12

Cited by 92 publications

(58 citation statements)

References 34 publications

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“…T he introduction of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) in the clinical microbiology laboratories is changing the approach to bacterial and fungal identification (1)(2)(3)(4). In particular, several studies have already demonstrated the reliability of MALDI-TOF MS in the rapid identification of yeasts in different clinical settings (5-7), evidencing its cost-effectiveness in allowing the initiation of species-targeted antifungal therapy (7)(8)(9).…”

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confidence: 99%

Comparative Evaluation of the Bruker Biotyper and Vitek MS Matrix-Assisted Laser Desorption Ionization–Time Of Flight (MALDI-TOF) Mass Spectrometry Systems for Identification of Yeasts of Medical Importance

et al. 2013

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We report the first comparative evaluation between the Bruker Biotyper MS (BMS) and the Vitek MS (VMS) for the identification of yeasts. The rate of correct identifications at the species level was comparable using the commercial databases (89.8% versus 84.3%; P ‫؍‬ 0.712), but higher for BMS using an in-house-extended database (100% versus 84.3%; P ‫؍‬ 0.245). Importantly, the rate of misidentification was significantly higher for VMS (1% versus 12.1%; P < 0.0001), including the rate of major errors (0% versus 4.5%; P ‫؍‬ 0.0036). The introduction of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) in the clinical microbiology laboratories is changing the approach to bacterial and fungal identification (1-4). In particular, several studies have already demonstrated the reliability of MALDI-TOF MS in the rapid identification of yeasts in different clinical settings (5-7), evidencing its cost-effectiveness in allowing the initiation of species-targeted antifungal therapy (7-9). To date, four MALDI-TOF MS systems are commercially available: the Microflex LT Biotyper (Bruker Daltonics, Bremen, Germany) (BMS), the Saramis system (bio-Mérieux, Marcy l'Etoile, France), the Vitek MS system (bioMérieux, Marcy l'Etoile, France) (VMS), and, very recently, the Andromas system (Andromas, Paris, France). Several comparative studies have already been performed using the most common systems (BMS and VMS), but, to the best of our knowledge, they have focused only on the identification of bacteria (10-13). Only very recently was a comparative study on yeasts performed using BMS and Saramis (bio-Mérieux, Marcy l'Etoile, France), the previously distributed version of VMS (14). In the present study, we evaluated the ability of BMS and VMS to identify a broad panel of yeasts of medical interest.One hundred ninety-seven isolates from different human samples, previously identified by conventional biochemical techniques or by sequencing the internal transcribed spacer 1 (ITS1) and ITS2 regions, were blindly identified using the two systems. In order to minimize the risk of misidentification related to the use of incomplete and error-filled public databases (15), the sequences obtained were compared to reference data available in two databases: GenBank, searched by using the nucleotide BLAST tool (blast.ncbi.nlm.nih.gov), and the CBS (Centraalbureau voor Schimmelcultures) yeast database (www.cbs.knaw.nl). The panel included 157 (79.7%) isolates belonging to 30 Candida or Candida-related species (Table 1), and 40 (20.3%) isolates belonging to 15 non-Candida species (Table 2). Before processing for MS identification, each isolate was cultured on Sabouraud dextrose (Kima, Padua, Italy) agar and incubated for 24 h at 35°C. For BMS, proteins were extracted as recommended by the manufacturer. Briefly, a loopful of yeasts was suspended in one volume of water and three volumes of absolute ethanol, and after centrifugation, the pellets were processed with an equal amount of formic acid and acetonitrile ...

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confidence: 99%

Comparative Evaluation of the Bruker Biotyper and Vitek MS Matrix-Assisted Laser Desorption Ionization–Time Of Flight (MALDI-TOF) Mass Spectrometry Systems for Identification of Yeasts of Medical Importance

et al. 2013

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“…Discriminatory peaks have been detected suggesting a direct link between infectious-related protease activity and a sepsis-specific diagnostic pattern for discrimination of patients with SIRS [30] . An interesting example is represented by the detection of the staphylococcal delta-toxin which was found associated with the acute infection [31] .…”

Section: Detection Of Virulence Factorsmentioning

confidence: 99%

“…The LC-ESI-QqQ in selected or multiple reactions way has been used for routine detection of small molecules including metabolites and drugs [31] . More recently, the LC-ESI-QqQ has been suggested as a replacement for classical ELISAs for the quantitation of proteins in complex matrices [98] .…”

Section: Other Technologiesmentioning

confidence: 99%

Mass spectrometry: a revolution in clinical microbiology?

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Dunyach-Rémy

et al. 2012

Clinical Chemistry and Laboratory Medicine (CCLM)

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Recently, different bacteriological laboratory interventions that decrease reporting time have been developed. These promising new broad-based techniques have merit, based on their ability to identify rapidly many bacteria, organisms difficult to grow or newly emerging strains, as well as their capacity to track disease transmission. The benefit of rapid reporting of identification and/or resistance of bacteria can greatly impact patient outcomes, with an improvement in the use of antibiotics, in the reduction of the emergence of multidrug resistant bacteria and in mortality rates. Different techniques revolve around mass spectrometry (MS) technology: matrix-assisted laser desorption ionization time-offlight mass spectrometry (MALDI-TOF MS), PCR combined with electrospray ionization-mass spectrometry (PCR/ESI-MS), iPLEX MassArray system and other new evolutions combining different techniques. This report emphasizes the (r)evolution of these technologies in clinical microbiology.

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“…Our isolate could not be identified beyond the genus level using this approach since no SuperSpectrum specific for P. glucanolyticus was, at the time, included in the RUO database. With the Vitek MS IVD system, the isolate was also only identified to the genus level using the Advanced Spectra Classifier approach, which represents an extension of the SuperSpectrum approach (28). Our isolate could be identified beyond the genus level with the Bruker Biotyper system and the Vitek MS RUO system when the compare mode was used.…”

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confidence: 99%

“…The two major commercial MALDI-TOF MS systems are the Bruker Biotyper and the bioMérieux Vitek MS systems (28). The databases of both systems contain mass spectra of P. glucanolyticus.…”

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Cardiac Device-Related Endocarditis Caused by Paenibacillus glucanolyticus

et al. 2013

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cWe report the first case of Paenibacillus glucanolyticus infection in a 65-year-old patient with type 2 diabetes who developed a cardiac device-related endocarditis. The identification of the isolate was performed using phenotypic methods, including mass spectrometry-based methods, and 16S rRNA gene sequencing. CASE REPORT In November 2012, a 65-year-old woman with type 2 diabetes was admitted to the University Hospital Center of Nancy because of a localized pacemaker pocket infection. The pacemaker was implanted 12 years before because of the patient's high-degree atrioventricular block. The generator was replaced in 2010. Since January 2012, the patient had reported signs of inflammation at the site of pacemaker implantation. In October 2012, the patient, who was afebrile, was admitted to another hospital with local signs of inflammation at the generator pocket with skin fistulization and purulent discharge. Pocket abscess drainage was then performed, and a pus specimen was sent to the local laboratory. The patient, who was allergic to penicillin, was treated with trimethoprim-sulfamethoxazole and erythromycin for 10 days. Gram staining of the pus sample did not reveal any organism, while a rod-shaped bacterium that appeared to be Gram negative was obtained by culture. This isolate was identified first as Cronobacter sakazakii (Vitek 2 GN card; bioMérieux, Marcy l'Etoile, France) and finally as Aeromonas salmonicida (API ID 32GN kit; bioMérieux).On admission to our hospital, the patient was afebrile, and physical examination revealed an inflammatory pacemaker pocket with a purulent discharge. No other symptoms were noted. The white blood cell count was 13.8 ϫ 10 9 /liter with 62% neutrophils. The serum C-reactive protein level was normal, while the serum procalcitonin level was slightly elevated. Transesophageal echocardiography revealed a hyperechogenic mass on the distal portion of the right atrial lead compatible with a vegetation. The patient underwent implantation of an epicardial pacemaker prior to percutaneous removal of the generator and the atrial and ventricular leads. The presence of a vegetation attached to the atrial lead was confirmed macroscopically. The patient was treated with ceftriaxone (2 g intravenously [i.v.] once a day) for 42 days. Four sets of blood cultures were drawn before the onset of antibiotic therapy. The leads as well as a pus sample obtained from the pacemaker pocket were sent for microbiological analysis.Specimens taken during the intervention were processed using standard methods and inoculated onto tryptic soy agar with 5% sheep blood (bioMérieux) incubated at 37°C in air and brucella blood agar with hemin and vitamin K1 (Becton, Dickinson, Le Pont de Claix, France) incubated at 37°C anaerobically. In addition, brain heart infusion and Rosenow broths (Bio-Rad, Marnesla-Coquette, France) were inoculated and incubated at 37°C, respectively, in air and anaerobically. Part of the vegetation embedding the atrial lead was also sampled for broad-range bacterial 16S rRNA gene PCR....

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Biomedical Mass Spectrometry in Today's and Tomorrow's Clinical Microbiology Laboratories

Cited by 92 publications

References 34 publications

Comparative Evaluation of the Bruker Biotyper and Vitek MS Matrix-Assisted Laser Desorption Ionization–Time Of Flight (MALDI-TOF) Mass Spectrometry Systems for Identification of Yeasts of Medical Importance

Comparative Evaluation of the Bruker Biotyper and Vitek MS Matrix-Assisted Laser Desorption Ionization–Time Of Flight (MALDI-TOF) Mass Spectrometry Systems for Identification of Yeasts of Medical Importance

Mass spectrometry: a revolution in clinical microbiology?

Cardiac Device-Related Endocarditis Caused by Paenibacillus glucanolyticus

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