Biomedical Membrane of Fish Collagen/Gellan Gum Containing Bone Graft Materials

Abstract

The development of a guided bone regeneration (GBR) membrane with non-mammalian fish collagen has the advantage of low risk for transmission of infectious diseases in tissue regeneration. In this work, a fish collagen/gellan gum and bone graft material (FC/GG-BGM) composite GBR membrane were fabricated through solution blending and casting procedures in a vacuum. The membranes were characterized using Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), scanning electron microscopy observation (SEM), and atomic force microscope (AFM) analyses. FT-IR results suggested that ionic interactions were formed between FC and GG both in composite powder and membranes. In vivo experiments showed that these FC/GG-BGM composite membranes could generate osteoblast minerals and promote loose bone calcification, thus accelerating bone regeneration. At 2 weeks, the defected site of rats treated with FC/GG-BGM membrane (0.377 ± 0.012 mm3) showed higher regeneration than that of rats treated with the bovine collagen membrane (0.290 ± 0.015 mm3) and control rats without membrane (0.160 ± 0.008 mm3). Compared with bovine collagen membrane, the FC/GG-BGM composite membrane displays better bone regeneration ability. Therefore, FC/GG-BGM composite membrane is suitable as a GBR membrane for bone regeneration.

Bioactive Scaffolds as a Promising Alternative for Enhancing Critical-Size Bone Defect Regeneration in the Craniomaxillofacial Region

“…In preclinical studies, Col sponges were able to accelerate bone healing in cranial CSDs in rats. − The most frequent source of Col has been cows and pigs; however, new sources, such as fishes and marine organisms, have been recently proposed to overcome religious issues and potential transmission of pathogens . For example, scaffolds made with fish Col mixed with gellan gum and bovine bone particles showed better osteoinductive properties than bovine Col membranes in CSDs in rats . Col mats’ mechanical properties and osteoinductivity have been enhanced by adding calcium phosphate (CaP) or apatite by in vitro mineralization.…”

Recent Progress in Stimuli-Responsive Hydrogels Application for Bone Regeneration

“…These defects can be either small or large, and they always require an efficient and secure treatment to restore bone tissue. Bone grafting has traditionally been the therapy of choice for bone abnormalities [3]. The restricted availability of autologous bone grafts [3], high treatment costs [4], danger of immunological rejection [5], difficulties in bone handling technologies [6], and the possibility of infection and consequences [7] have all contributed to the limited success of bone transplantation [3][4][5][6][7].…”

“…Bone grafting has traditionally been the therapy of choice for bone abnormalities [3]. The restricted availability of autologous bone grafts [3], high treatment costs [4], danger of immunological rejection [5], difficulties in bone handling technologies [6], and the possibility of infection and consequences [7] have all contributed to the limited success of bone transplantation [3][4][5][6][7]. The current state of the art in treating bone defects is inadequate to provide adequate care for patients [8].…”

The efficacy of a semi-resorbable membrane based on silk fibroin-Glycerol on bone regeneration in rabbit calvarial defects compared to a commercial collagen membrane

“…8 and some of them are approved in some countries for clinical use such as a wound dressing and a tympanic membrane. [9][10][11][12] Silk fibroin, extracted from silk fibers, can be constructed into films, sheets, scaffolds, and filaments. The material can be soft to super-hard due to its high malleability and rigidity.…”

“…And there are many in vitro studies that reported the potential of clinical use of fish collagen. [9][10][11] Recently, silk fibroin and fish collagen-based materials with a glycerol plasticizer had been fabricated and characterized to overcome the brittleness and low mechanical strength of silk fibroin material for a barrier membrane and to diminish religious restrictions from the mammal sources. 20 The in vitro study showed that the silk fibroin-glycerol and silk fibroin-glycerol-collagen membranes had good physical and mechanical properties with high stiffness 1.5-2.2 times that of the collagen membrane but still yielded sufficient elongation when compared to the collagen membrane, particularly the silk fibroin-glycerolcollagen.…”