Biomimetic 3D-printed PCL scaffold containing a high concentration carbonated-nanohydroxyapatite with immobilized-collagen for bone tissue engineering: enhanced bioactivity and physicomechanical characteristics

Moghaddaszadeh, Ali; Seddiqi, Hadi; Najmoddin, Najmeh; Ravasjani, Sonia Abbasi; Klein‐Nulend, Jenneke

doi:10.1088/1748-605x/ac3147

Cited by 23 publications

(19 citation statements)

References 93 publications

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“…No impurity is observed in the XRD peaks. The most intense peak (101) was chosen to calculate the crystallite size according to Scherrer's Equation, 35 which gave an estimated average value of about 33 nm.…”

Section: Resultsmentioning

confidence: 99%

“…The XRD diffraction peaks at around 2θ values of 32 , 34 , 36 , 48 , 57 , 63 , 66 , 68 , 69 , 73 , and 77 respectively correspond to the (100), ( 002), ( 101), ( 102), ( 110), ( 103), ( 200), ( 112), ( 201 impurity is observed in the XRD peaks. The most intense peak (101) was chosen to calculate the crystallite size according to Scherrer's Equation, 35 which gave an estimated average value of about 33 nm.…”

Section: Synthesis and Characterization Of Clay@ Znomentioning

confidence: 99%

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Enhancing mechanical and antibacterial properties of polycaprolactone nanocomposite nanofibers using decorated clay with ZnO nanorods

Maghfoori

Najmoddin

Pezeshki‐Modaress

2022

J of Applied Polymer Sci

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It is urgently necessitate to develop a construct with desirable mechanical properties and antibacterial activity for biomedical applications. Zinc oxide (ZnO) has emerged as a promising antibacterial inorganic agent for the improvement of biopolymer scaffold properties. However, its agglomeration/aggregation has an adverse effect on its performance. In the present work, clay platelets have served as a substrate for decoration of high density, well‐distributed ZnO nanorods via in‐situ synthesis. Then, polycaprolactone (PCL) nanofibers containing 1%, 2%, 4%, and 6% ZnO nanorods decorated on clay platelets (Clay@ ZnO) are fabricated using the electrospinning technique. The tensile strength and modulus of the PCL scaffold are significantly enhanced by increasing Clay@ ZnO content, thanks to the presence of well‐distributed ZnO rods on exfoliated clay platelets. However, the maximum elongation at break is observed for PCL/1% (Clay@ ZnO). Disc diffusion method reveals that good coverage of clay platelets with ZnO nanorods boosts the antibacterial performance of ZnO against both Staphylococcus aureus and Escherichia coli bacteria strains. Moreover, incorporation of Clay@ ZnO powders into the PCL scaffold considerably enhances its antibacterial activity. Cell culturing assay demonstrates the favorable cytocompatibility of prepared nanocomposite nanofibers by promoting cell attachment and proliferation. Such engineered nanocomposite has a high potential to utilize in biomedical applications.

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Section: Resultsmentioning

confidence: 99%

Section: Synthesis and Characterization Of Clay@ Znomentioning

confidence: 99%

Enhancing mechanical and antibacterial properties of polycaprolactone nanocomposite nanofibers using decorated clay with ZnO nanorods

Maghfoori

Najmoddin

Pezeshki‐Modaress

2022

J of Applied Polymer Sci

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“…Therefore, the increased Mepe expression might be related to the improved cell spreading on the OCP coating containing κ-carrageenan. ALP activity and matrix mineralization are important for bone regeneration ( Moghaddaszadeh et al, 2021 ). We found that κ-carrageenan in the OCP coating enhanced ALP activity and ALP protein in the short term (3, 7 days), as well as enhanced matrix mineralization in the long term (21 days) in pre-osteoblasts, suggesting stimulation of bone formation.…”

Section: Discussionmentioning

confidence: 99%

Kappa-carrageenan-Functionalization of octacalcium phosphate-coated titanium Discs enhances pre-osteoblast behavior and osteogenic differentiation

Cao

Jin

et al. 2022

Front. Bioeng. Biotechnol.

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Bioactive coatings are promising for improving osseointegration and the long-term success of titanium dental or orthopaedic implants. Biomimetic octacalcium phosphate (OCP) coating can be used as a carrier for osteoinductive agents. κ-Carrageenan, a highly hydrophilic and biocompatible seaweed-derived sulfated-polysaccharide, promotes pre-osteoblast activity required for bone regeneration. Whether κ-carrageenan can functionalize OCP-coating to enhance osseointegration of titanium implants is unclear. This study aimed to analyze carrageenan-functionalized biomimetic OCP-coated titanium structure, and effects of carrageenan functionalization on pre-osteoblast behavior and osteogenic differentiation. Titanium discs were coated with OCP/κ-carrageenan at 0.125–2 mg/ml OCP solution, and physicochemical and biological properties were investigated. κ-Carrageenan (2 mg/ml) in the OCP coating of titanium discs decreased the pore size in the sheet-like OCP crystal by 41.32%. None of the κ-carrageenan concentrations tested in the OCP-coating did affect hydrophilicity. However, κ-carrageenan (2 mg/ml) increased (1.26-fold) MC3T3-E1 pre-osteoblast spreading at 1 h i.e., κ-Carrageenan in the OCP-coating increased pre-osteoblast proliferation (max. 1.92-fold at 2 mg/ml, day 1), metabolic activity (max. 1.50-fold at 2 mg/ml, day 3), and alkaline phosphatase protein (max. 4.21-fold at 2 mg/ml, day 3), as well as matrix mineralization (max. 5.45-fold at 2 mg/ml, day 21). κ-Carrageenan (2 mg/ml) in the OCP-coating increased gene expression of Mepe (4.93-fold) at day 14, and Runx2 (2.94-fold), Opn (3.59-fold), Fgf2 (3.47-fold), Ocn (3.88-fold), and Dmp1 (4.59-fold) at day 21 in pre-osteoblasts. In conclusion, κ-carrageenan modified the morphology and microstructure of OCP-coating on titanium discs, and enhanced pre-osteoblast metabolic activity, proliferation, and osteogenic differentiation. This suggests that κ-carrageenan-functionalized OCP coating may be promising for in vivo improvement of titanium implant osseointegration.

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“…Bone tissue engineering requires three-dimensional (3D)bioactive scaffolds mimicking bone in their structural, chemical, and mechanical properties in order to support cell adhesion, proliferation, and differentiation (Moghaddaszadeh et al, 2021). 3D printing is a favorable technology for producing bone scaffolds with controlled physical and mechanical properties, as well as a hierarchical structure analogous to the bone matrix (Entezari et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Sulfated carboxymethyl cellulose and carboxymethyl κ-carrageenan immobilization on 3D-printed poly-ε-caprolactone scaffolds differentially promote pre-osteoblast proliferation and osteogenic activity

Abbasi-Ravasjani

Seddiqi

Moghaddaszadeh

et al. 2022

Front. Bioeng. Biotechnol.

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The lack of bioactivity in three-dimensional (3D)-printing of poly-є-caprolactone (PCL) scaffolds limits cell-material interactions in bone tissue engineering. This constraint can be overcome by surface-functionalization using glycosaminoglycan-like anionic polysaccharides, e.g., carboxymethyl cellulose (CMC), a plant-based carboxymethylated, unsulfated polysaccharide, and κ-carrageenan, a seaweed-derived sulfated, non-carboxymethylated polysaccharide. The sulfation of CMC and carboxymethylation of κ-carrageenan critically improve their bioactivity. However, whether sulfated carboxymethyl cellulose (SCMC) and carboxymethyl κ-carrageenan (CM-κ-Car) affect the osteogenic differentiation potential of pre-osteoblasts on 3D-scaffolds is still unknown. Here, we aimed to assess the effects of surface-functionalization by SCMC or CM-κ-Car on the physicochemical and mechanical properties of 3D-printed PCL scaffolds, as well as the osteogenic response of pre-osteoblasts. MC3T3-E1 pre-osteoblasts were seeded on 3D-printed PCL scaffolds that were functionalized by CM-κ-Car (PCL/CM-κ-Car) or SCMC (PCL/SCMC), cultured up to 28 days. The scaffolds’ physicochemical and mechanical properties and pre-osteoblast function were assessed experimentally and by finite element (FE) modeling. We found that the surface-functionalization by SCMC and CM-κ-Car did not change the scaffold geometry and structure but decreased the elastic modulus. Furthermore, the scaffold surface roughness and hardness increased and the scaffold became more hydrophilic. The FE modeling results implied resilience up to 2% compression strain, which was below the yield stress for all scaffolds. Surface-functionalization by SCMC decreased Runx2 and Dmp1 expression, while surface-functionalization by CM-κ-Car increased Cox2 expression at day 1. Surface-functionalization by SCMC most strongly enhanced pre-osteoblast proliferation and collagen production, while CM-κ-Car most significantly increased alkaline phosphatase activity and mineralization after 28 days. In conclusion, surface-functionalization by SCMC or CM-κ-Car of 3D-printed PCL-scaffolds enhanced pre-osteoblast proliferation and osteogenic activity, likely due to increased surface roughness and hydrophilicity. Surface-functionalization by SCMC most strongly enhanced cell proliferation, while CM-κ-Car most significantly promoted osteogenic activity, suggesting that surface-functionalization by CM-κ-Car may be more promising, especially in the short-term, for in vivo bone formation.

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Biomimetic 3D-printed PCL scaffold containing a high concentration carbonated-nanohydroxyapatite with immobilized-collagen for bone tissue engineering: enhanced bioactivity and physicomechanical characteristics

Cited by 23 publications

References 93 publications

Enhancing mechanical and antibacterial properties of polycaprolactone nanocomposite nanofibers using decorated clay with ZnO nanorods

Enhancing mechanical and antibacterial properties of polycaprolactone nanocomposite nanofibers using decorated clay with ZnO nanorods

Kappa-carrageenan-Functionalization of octacalcium phosphate-coated titanium Discs enhances pre-osteoblast behavior and osteogenic differentiation

Sulfated carboxymethyl cellulose and carboxymethyl κ-carrageenan immobilization on 3D-printed poly-ε-caprolactone scaffolds differentially promote pre-osteoblast proliferation and osteogenic activity

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