Biomimetic Chemical Sensors Using Nanoelectronic Readout of Olfactory Receptor Proteins

Abstract

We have designed and implemented a practical nanoelectronic interface to G-protein coupled receptors (GPCRs), a large family of membrane proteins whose roles in the detection of molecules outside eukaryotic cells make them important pharmaceutical targets. Specifically, we have coupled olfactory receptor proteins (ORs) with carbon nanotube transistors. The resulting devices transduce signals associated with odorant binding to ORs in the gas phase under ambient conditions and show responses that are in excellent agreement with results from established assays for OR–ligand binding. The work represents significant progress on a path toward a bioelectronic nose that can be directly compared to biological olfactory systems as well as a general method for the study of GPCR function in multiple domains using electronic readout.

Rapid green synthesis of silver nanoparticles from Chrysanthemum indicum L and its antibacterial and cytotoxic effects: an in vitro study

“…The nature and unique properties of nanomaterials have a wide range of applications in biosensors, tissue engineering, deoxyribonucleic acid (DNA) modification, drug delivery systems, cosmetics, and medical devices. [1][2][3][4][5] Silver NPs (AgNPs) are well known biocidal substances that can be incorporated as antimicrobial agents in pharmacology, veterinary medicine, implants, wound dressings, and topical ointments.…”

Ultrasensitive Flexible Graphene Based Field-Effect Transistor (FET)-Type Bioelectronic Nose

“…0.04 fM (signal-to-noise ratio: 4.2), which is 2 to 5 orders of magnitude more sensitive than previously reported odorant sensors. [6][7][8][9]58 Moreover, the B-noses had rapid response time of less than 1 s and showed an instantaneous signal increase at all concentrations (0.04 fM to 400 pM). This is explained that the olfactory signaling in sensory transduction is relatively fast, in the order of milliseconds ( Figure S9).…”

Properties and Customization of Sensor Materials for Biomedical Applications

“…Thanks to their high conductivity and large surface-to-volume ratio, small changes on the conformation or displacement of the immobilized bioreceptors can be detected (243,244). This transduction mechanism has been reported in field-effect transistors (FET) (2,69,245,246) and may provide the basis of future devices like sensor-based bioelectronic noses and tongues (114,236,247), although, at present, the response patterns obtained by these devices are not stable over time (2). This is a serious limitation in bioelectronic tongues and noses, although their potential to extract complex information from the sensor array seems very powerful (2).…”