Biomimetic Growth of Hydroxyapatite on SiO2 Microspheres to Improve Its Biocompatibility and Gentamicin Loading Capacity

Abstract

The interest in multifunctional biomaterials to be implanted are also able to release drugs that reduce pain and inflammation or prevent a possible infection has increased. Bioactive materials such as silica (SiO2) containing surface silanol groups contribute to the nucleation and growth of hydroxyapatite (HAp) in a physiological environment. Regarding biocompatibility, the spherical shape of particles is the desirable one, since it does not cause mechanical damage to the cell membrane. In this work, the synthesis of SiO2 microspheres was performed by the modified Stöber method and they were used for the biomimetic growth of HAp on their surface. The effect of the type of surfactant (sodium dodecyl sulphate (SDS), cetyltrimethylammonium bromide (CTAB), and polyethylene glycol (PEG)), and heat treatment on the morphology and size of SiO2 particles was investigated. Monodisperse, spherical-shaped SiO2 microparticles with an average particle size of 179 nm, were obtained when using PEG (SiO2-PEG). The biomimetic growth of HAp was performed on this sample to improve its biocompatibility and drug-loading capacity using gentamicin as a model drug. Biomimetic growth of HAp was confirmed by FTIR-ATR, SEM-EDX and TEM techniques. SiO2-PEG/HAp sample had a better biocompatibility in vitro and gentamicin loading capacity than SiO2-PEG sample.

Osteogenic Induction with Silicon Hydroxyapatite Using Modified Autologous Adipose Tissue-Derived Stromal Vascular Fraction: In Vitro and Qualitative Histomorphometric Analysis

“…The results are expressed as the hemolytic ratio, labelled in three categories, with <2% indicating non-hemolytic, 2–5% slightly non-hemolytic, and >5% hemolytic. In our study, the observations were in agreement with previously reported results [ 41 , 49 , 50 ]. A maximum hemolysis value of 3.66% in Sample 2 showed increased thrombogenicity, which may be the result of greater blood–surface interactions due to the ionic groups in Si-HA interacting with certain blood components ( Table 4 ) [ 51 ].…”

Hydroxyapatite-based carriers for tumor targeting therapy

“…As the surface of SiO 2 contains silanol groups, it is conducive to the nucleation and growth of HAP on the surface of SiO 2 in the physiological environment. 96 The results conrmed that SiO 2 /HAP samples had better biocompatibility and drug loading ability in vitro than SiO 2 samples. Adding a biocompatible HAP microsheet layer to the TiO 2 /polymer hybrid layer, can produce a loose lm structure and enhance the photocatalytic surface, which can help kill more microorganisms and improve the antibacterial performance.…”