Biomimetic hydroxyapate/polydopamine composites with good biocompatibility and efficiency for uncontrolled bleeding

Abstract

Uncontrolled bleeding is thought to be the most deadly cause of pre‐hospital, traffic, and military accidents death. However, the popular commercial hemostats can only realize the hemostasis of mild bleeding. Therefore, we developed polydopamine (PDA) composite materials (PMs), which applied hydroxyapatite as the parent body. The PMs were produced via lyophilization and functionalized with amino, phenol hydroxyls groups, which endowed hydrophobicity to materials. This ensured a high aggregation ability of blood cells to the PMs and they were tested to be as high as 300% compared with the negative control group. The clotting time was shortened to 79.7% compared with the usually used commercial hemostat (Celox) in the test of in vitro hemostasis. Through the results of PT and APTT tests, blood coagulation index test, and the analysis of intracellular Ca2+ activation, we further understood the mechanism of the hemostasis of the materials, which explained the low blood loss and quick coagulation time of the PM hemostats in detail. Besides, the low hemolysis and cytotoxicity of the PMs suggested the good biocompatibility of the hemostats, which was further proved by the regular morphology maintained by erythrocytes in the hemolysis tests. The study of nanoscale composites led the research for the methods of hemostasis.

Synergistic surface modification of anhydrous calcium sulfate by polydopamine/oleylamine and its application in reinforced HDPE composites

“…Compared with CSA‐OH, new peaks appear at 1626 and 1431 cm −1 in mCSA, which are attributed to the specific structure of oleylamine double bond and polyindole in the reaction of oleylamine and polydopamine, 28 and a new peak appears at 871 cm −1 , which belongs to the hydrogen‐oxygen peak in polydopamine, 29 there are new peaks appearing at 2920 and 2850 cm −1 , which belong to the hydrocarbon peaks of aromatic and oleylamine long chains. In addition, the peak at 3432 cm −1 in Figure 1D becomes larger and broader, indicating that the interaction between polydopamine and CSA may be through hydrogen bonds, which comes from the phenolic hydroxyl groups and amino groups on PDA 30 . The FT‐IR results show that polydopamine could be successfully combined with oleylamine and deposited on the surface of calcium sulfate.…”

“…In addition, the peak at 3432 cm À1 in Figure 1D becomes larger and broader, indicating that the interaction between polydopamine and CSA may be through hydrogen bonds, which comes from the phenolic hydroxyl groups and amino groups on PDA. 30 The FT-IR results show that polydopamine could be successfully combined with oleylamine and deposited on the surface of calcium sulfate.…”

Recent Development and Applications of Polydopamine in Tissue Repair and Regeneration Biomaterials

“…52 Gong et al designed a hydroxyapatite hemostatic material with PDA. 53 They work out that the best ratio of nano hydroxyapatite and DOPA hydrochloride is 20:1. The hemostatic capacity between a popular commercial production Celox and this composite was studied.…”

Mussel-inspired collagen-hyaluronic acid composite scaffold with excellent antioxidant properties and sustained release of a growth factor for enhancing diabetic wound healing

“…It has been proved by Liu et al. that pDA has good biocompatibility with low cytotoxicity and stability in vivo [ 11 ]. And the free radical scavenging ability of pDA-NPs has been convinced by Zhao et al.…”