Biomimetic Joint Paint for Efficient Cartilage Repair by Simultaneously Regulating Cartilage Degeneration and Regeneration in Pigs

Abstract

Irregular partial-thickness cartilage defect is a common pathogenesis of osteoarthritis (OA) with no available treatment in clinical practice. Currently, cartilage tissue engineering is only suitable for a limited area of full-thickness cartilage defect. Here, we design a biomimetic joint paint for the intractable partial-thickness cartilage defect repair. The joint paint, composed of a bridging layer of chondroitin sulfate and a surface layer of gelatin methacrylate with hyaluronic acid, can quickly and tightly adhere to the cartilage defect by light activation. Being treated by the joint paint, the group of rabbit and pig models with partial-thickness cartilage defects showed a restoration of a smooth cartilage surface and the preservation of normal glycosaminoglycan content, whereas the untreated control group exhibited serious progressive OA development. This paint treatment functions by prohibiting chondrocyte apoptosis, maintaining chondrocyte phenotype, and preserving the content of glycosaminoglycan in the partial-thickness cartilage defects. These findings illustrated that the biomimetic joint paint is an effective and revolutionary therapeutics for the patients with noncurable partial-thickness cartilage defects.

Human urine-derived stem cell exosomes delivered via injectable GelMA templated hydrogel accelerate bone regeneration

“…Combined with the introduction of photocross-linked HAMA hydrogels and ultrasonically emulsified nHAP, the mechanical properties of the composite hydrogels were significantly reinforced. Besides, the hydrogen proton 1 H NMR spectra of the composite hydrogels demonstrated that the methacrylated gelatin and hyaluronic acid methacrylate successfully photocrosslinked with each other and simultaneously retained –CH CH–CH 3 or –CH CH 2 functional groups, which is consistent with the published literature [ 29 , 33 , 34 ]. The physiological roles of USC EXOs implicated in osteogenesis, angiogenesis, and anti-inflammation have been demonstrated [ 11 , 35 ].…”

Dual role of injectable curcumin-loaded microgels for efficient repair of osteoarthritic cartilage injury

“…Surprisingly, the long-term toxicity profile of local distribution ( Saifi et al, 2018 ) as well as the body wide non-targeting infiltration ( Mohammadpour et al, 2019 ; Wu et al, 2019 ) of nanoparticles are still one of the most important concerns when drug-loaded nano-emusions or nanoparticles were intraarticularly injected. On the contrary, hydrogels spheroids at micro-scale were proven to be more available for the direct release of cells, growth factors and drugs ( Annamalai et al, 2019 ; Volpatti et al, 2021 ), and could provide as a dense barrier for inflammatory erosion and chondrocyte apoptosis ( Wei et al, 2021 ; Han et al, 2022 ).…”

Knowledge domain and hotspots concerning photosensitive hydrogels for tissue engineering applications: A bibliometric and visualized analysis (1996-2022)

“…Since then, the university has been committed to the study of photosensitive hydrogels for TE applications. Recently, the research group of Ouyang from Zhejiang Univ developed a biomimetic joint paint containing GelMA with hyaluronic acid which can be rapidly activated by light for the treatment of cartilage defect ( Wei et al, 2021 ). However, as the two countries that published the most papers, the United States and China did not cooperate closely in this field.…”