Biomimetic macroporous PEG hydrogels as 3D scaffolds for the multiplication of human hematopoietic stem and progenitor cells

Raic, Annamarija; Rödling, Lisa; Kalbacher, Hubert; Lee-Thedieck, Cornelia

doi:10.1016/j.biomaterials.2013.10.038

Cited by 169 publications

(163 citation statements)

References 53 publications

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“…Several different approaches to culture and investigate HSCs in vitro using 3D systems have been tested up until now: HSCs †, § have been encapsulated in hydrogels made of natural (e.g., collagen, hyaluronic acid) or synthetic (e.g., polyacrylates) monomers; 48,50,52 Other approaches included seeding of HSCs † onto scaffolds made of fibres or fibre meshworks, 51 as well as culturing HSCs †, ‡ ‡ in macroporous scaffolds that resemble the spongy architecture of trabecular bones 49,[181][182][183] (these host the red, blood-forming bone marrow); Furthermore, microwell-arrays have been used as quasi-or pseudo-3D environments for HSCs § §, ¶ ¶ in order to delineate signals and parameters that are present in the HSC niche. [184][185][186][187] All of these studies showed that HSCs respond and act differently in 3D materials than in standard 2D culture, backing the assumption that conventional 2D culture is a highly artificial situation for HSCs, which may even induce 'unnatural' cell behaviour.…”

Section: D Versus 2dmentioning

confidence: 99%

“…Neither stromal support in 2D nor 3D scaffolds alone was able to support HSC marker maintenance to this degree. 49 Each of the afore-mentioned approaches for HSC 3D culture has its advantages and disadvantages -and each system can best imitate a different aspect of the natural 3D situation. A combination of the different approaches and techniques might be a promising future approach to mimic the natural niche as realistically as possible.…”

Section: D Versus 2dmentioning

confidence: 99%

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Biophysical regulation of hematopoietic stem cells

Lee-Thedieck

Spatz

2014

Biomater. Sci.

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Section: D Versus 2dmentioning

confidence: 99%

Section: D Versus 2dmentioning

confidence: 99%

Biophysical regulation of hematopoietic stem cells

Lee-Thedieck

Spatz

2014

Biomater. Sci.

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“…Tissue-engineered approaches to the HSC niche are frequently based on hydrogels [32,[37][38][39][40]. However, the use of solid scaffolds with higher mechanical properties allows more complex approaches.…”

Section: Discussionmentioning

confidence: 99%

“…Mineralised osteoblasts enhanced expansion of haematopoietic cells in 2D cultures, while myeloid [9] and lymphoid [73] differentiation was induced. The incorporation of bmMSCs in hydrogels maintained primitive HSCs and induced differentiation of a sub-population [32,37].…”

Section: Discussionmentioning

confidence: 99%

Endosteal-like extracellular matrix expression on melt electrospun written scaffolds

et al. 2017

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Endosteal-like extracellular matrix expression on melt electrospun written scaffolds, Acta Biomaterialia (2016), doi: http://dx.doi.org/10. 1016/j.actbio.2016.12.040 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. models that mimic the endosteal microenvironment enable researchers to discover the causes and improve treatments for blood and immune-related diseases. The aim of this study was to establish a physiologically relevant in vitro model using 3D printed scaffolds to assess the contribution of human cells to the formation of a construct that mimics human endosteum. Melt electrospun written scaffolds were used to compare the suitability of primary human osteoblasts (hOBs) and placenta-derived mesenchymal stem cells (plMSCs) in ( when compared to HSCs maintained using tissue culture plastic. This 3D testing platform represents an endosteal bone-like platform and warrants future investigation for the maintenance and expansion of human HSCs.

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“…Raic et al [88] could show that by employing a salt leaching technique to prepare porous poly (ethylene glycol) diacrylate (PEGDA) hydrogels, HSCs from cord blood in co-culture with mesenchymal stromal cells from bone marrow had the strongest proliferative effect and that this effect was due to the 3D-environmentwhen compared to a 2D-system. Using collagen gels, with or without embedded MSCs, only HSCs from bone marrow could be shown to preserve stemness whereas HSCs from cord blood displayed increased proliferation and lineage commitment [89].…”

Section: Gel-based Systemsmentioning

confidence: 99%

Artificial Hematopoietic Stem Cell Niches-Dimensionality Matters

Gottwald¹

2017

ATROA

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Hematopoietic stem cell niches are perhaps the best described niche system in mammals. The niches themselves, as well as their cellular and structural constituents and factors that play a role in maintaining the niche structure and function, are being refined on a more or less daily basis. Despite this, it seems as if the more we know the harder it gets to mimic the in vivo-situation using in vitro-systems. This is due to the fact that hematopoiesis is a multi step maturation process leading to HSC heterogeneity. Subpopulations of HSCs and niche supporting cells can be defined depending on characteristics such as their potency of leading to successful reconstitution of sub lethally irradiated mice in serial transplantation experiments or, with less scientific impact, clonogenic assays. Since the bone marrow obviously provides all necessary information to maintain the stem cell pool constant and to adapt the number of blood cells according to physiologic needs, it has been the goal to engineer artificial niches that display at least one or several of the major characteristics of the in vivosituation to make use of these systems for not only fundamental research purposes but, moreover, also for clinical applications. This review will give an overview of approaches to engineering artificial hematopoietic niches with a focus on the complexity/dimensionality of the systems used.

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Biomimetic macroporous PEG hydrogels as 3D scaffolds for the multiplication of human hematopoietic stem and progenitor cells

Cited by 169 publications

References 53 publications

Biophysical regulation of hematopoietic stem cells

Biophysical regulation of hematopoietic stem cells

Endosteal-like extracellular matrix expression on melt electrospun written scaffolds

Artificial Hematopoietic Stem Cell Niches-Dimensionality Matters

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