Biomimetic Magnetite Nanoparticles as Targeted Drug Nanocarriers and Mediators of Hyperthermia in an Experimental Cancer Model

Oltolina, Francesca; Peigneux, Ana; Colangelo, Donato; Clemente, Nausicaa; D’Urso, Annarita; Valente, Guido; Iglesias, Guillermo R.; Jiménez-López, Concepción; Prat, María

doi:10.3390/cancers12092564

Cited by 39 publications

(44 citation statements)

References 81 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On one hand, their nano scale size makes them display a larger surface area that allows them to carry relatively large amounts of the relevant molecule. On the other hand, their magnetic properties allow an external guidance and/or concentration at the target site plus the combination of therapies, such as targeted drug delivery and magnetic hyperthermia [ 2 , 3 , 4 , 5 ].…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, the production of those BMNPs is eco-friendly and cost-effective [ 10 ]. MamC-mediated BMNPs have raised special interest, as they have shown in vitro to be effective drug nanocarriers and magnetic hyperthermia agents, both uncoated and coated by liposomes [ 4 , 5 , 11 , 12 , 13 ]. These BMNPs are larger than most commercial SPION and/or other biomimetic magnetites.…”

Section: Introductionmentioning

confidence: 99%

“…Additionally, these BMNPs have proven to be effective hyperthermia agents under the application of an alternate magnetic field (AMF) [ 4 , 5 , 12 , 14 ]. Temperature variations could interfere with basal metabolisms and homeostasis processes.…”

Section: Introductionmentioning

confidence: 99%

“…This effect is due to heat generation by magnetic energy dissipation from the single-domain particles caused by internal Néel fluctuations of the nanoparticle magnetic moment and external Brownian fluctuations following the application of an AMF [ 17 ]. Therefore, the use of magnetic nanoparticles able to be directed to the target and, once there, to locally increase the temperature is a promising alternative to treat cancer, being the optimum results when magnetic hyperthermia is combined with directed chemotherapy by using the magnetic nanoparticles as nanocarriers [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

“…Nevertheless, to maximize the effects of the directed chemotherapy and/or magnetic hyperthermia, it is important to facilitate the cellular uptake of the BMNPs [ 4 , 5 ]. While placing a magnet at the target site has been shown to facilitate internalization [ 3 , 5 ], other strategies need to be developed to further optimize BMNPs cellular uptake. Encapsulation of BMNPs becomes an attractive candidate to improve, not only internalization, but also biocompatibility and to prevent aggregation and oxidation.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Improving the Cellular Uptake of Biomimetic Magnetic Nanoparticles

et al. 2021

Self Cite

View full text Add to dashboard Cite

Magnetococcus marinus magnetosome-associated protein MamC, expressed as recombinant, has been proven to mediate the formation of novel biomimetic magnetic nanoparticles (BMNPs) that are successful drug nanocarriers for targeted chemotherapy and hyperthermia agents. These BMNPs present several advantages over inorganic magnetic nanoparticles, such as larger sizes that allow the former to have larger magnetic moment per particle, and an isoelectric point at acidic pH values, which allows both the stable functionalization of BMNPs at physiological pH value and the molecule release at acidic (tumor) environments, simply based on electrostatic interactions. However, difficulties for BMNPs cell internalization still hold back the efficiency of these nanoparticles as drug nanocarriers and hyperthermia agents. In the present study we explore the enhanced BMNPs internalization following upon their encapsulation by poly (lactic-co-glycolic) acid (PLGA), a Food and Drug Administration (FDA) approved molecule. Internalization is further optimized by the functionalization of the nanoformulation with the cell-penetrating TAT peptide (TATp). Our results evidence that cells treated with the nanoformulation [TAT-PLGA(BMNPs)] show up to 80% more iron internalized (after 72 h) compared to that of cells treated with BMNPs (40%), without any significant decrease in cell viability. This nanoformulation showing optimal internalization is further characterized. In particular, the present manuscript demonstrates that neither its magnetic properties nor its performance as a hyperthermia agent are significantly altered due to the encapsulation. In vitro experiments demonstrate that, following upon the application of an alternating magnetic field on U87MG cells treated with BMNPs and TAT-PLGA(BMNPs), the cytotoxic effect of BMNPs was not affected by the TAT-PLGA enveloping. Based on that, difficulties shown in previous studies related to poor cell uptake of BMNPs can be overcome by the novel nanoassembly described here.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Improving the Cellular Uptake of Biomimetic Magnetic Nanoparticles

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

From Micromagnetic to In Silico Modeling of Magnetic Nanodisks for Hyperthermia Applications

Manzin

Ferrero

Vicentini

2021

Advcd Theory and Sims

View full text Add to dashboard Cite

Magnetic nanodisks have been recently proposed as biomedical tools for therapeutics at the nanoscale level, with a special focus on hyperthermia for cancer cure. Here we present a detailed study of permalloy nanodisks to be used in alternative to superparamagnetic iron oxide nanoparticles, as efficient heating agents that release heat via magnetic hysteresis. A micromagnetic modeling analysis is carried out to identify sizes and ac field parameters that maximize the specific loss power (SLP), guaranteeing the fulfillment of biophysical constraints (Hergt–Dutz limit) and vortex state at remanence (reduced agglomeration effects). The highest SLP (790 W g−1) is found for 100 nm diameter and 20 nm thickness nanodisks, excited at a frequency of 75 kHz. Further analysis elucidates the influence of magnetostatic interactions and local nanodisk‐field orientation on the SLP of nanodisk clusters, which originate from the deposition in target tissues. At high concentrations, magnetostatic interactions can lead to a reduction of 40–50% in the hysteresis losses. From thermal simulations, we finally demonstrate that in a murine model temperature increments comparable to that obtained in calorimetric measurements under quasi‐adiabatic conditions can be achieved only by using an order of magnitude larger dosage of nanodisks, due to blood perfusion effects.

show abstract

The effect of the magnetically dead layer on the magnetization and the magnetic anisotropy of the dextran-coated magnetite nanoparticles

et al. 2022

View full text Add to dashboard Cite

We present a study on the magnetic behavior of dextran-coated magnetite nanoparticles (DM NPs) with sizes between 3 and 19 nm, synthesized by hydrothermal-assisted co-precipitation method.The decrease of saturation magnetization (M s ) with decreasing particle size has been modeled by assuming the existence of a spin-disordered layer at the particle surface, which is magnetically dead. Based on this core-shell model and taking into account the weight contribution of nonmagnetic coating layer (dextran) to the whole magnetization, the dead layer thickness (t) and saturation magnetization M s of the magnetic cores in our samples were estimated to be t = 6.8 Å and M s = 98.8 emu g ⁄ , respectively. The data of M s were analyzed using a law of approach to saturation, indicating an increase in effective magnetic anisotropy (K eff ) with decreasing the particle size as expected from the increased surface/volume ratio in small MNPs. The obtained K eff values were successfully modeled by including an extra contribution of dipolar interactions due to the formation of chain-like clusters of MNPs. The surface magnetic anisotropy (K s ) was estimated to be about K s = 1.04 × 10 5 J m 3 ⁄ . Our method provides a simple and accurate way to

show abstract

Biomimetic Magnetite Nanoparticles as Targeted Drug Nanocarriers and Mediators of Hyperthermia in an Experimental Cancer Model

Cited by 39 publications

References 81 publications

Improving the Cellular Uptake of Biomimetic Magnetic Nanoparticles

Improving the Cellular Uptake of Biomimetic Magnetic Nanoparticles

From Micromagnetic to In Silico Modeling of Magnetic Nanodisks for Hyperthermia Applications

The effect of the magnetically dead layer on the magnetization and the magnetic anisotropy of the dextran-coated magnetite nanoparticles

Contact Info

Product

Resources

About