Biomimetic Membranes with Aqueous Nanochannels. Phase Transitions and Oscillations

Kocherginsky, N.M.

doi:10.1134/s2517751621060111

Cited by 3 publications

(1 citation statement)

References 18 publications

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“…The accumulation of saturated long-chain FFAs in liver mitochondria incubated with divalent cations was confirmed previously [ 38 , 46 ], and their participation in Ca 2+ /H + and K + /H + exchange in the organelles was proposed [ 48 ]. Recent data also suggest that fatty acids in the presence of calcium ions can induce self-sustaining fluctuations in transmembrane voltage and current in biomimetic membranes without proteins [ 49 , 50 ].…”

Section: Discussionmentioning

confidence: 99%

The Short-Term Opening of Cyclosporin A-Independent Palmitate/Sr2+-Induced Pore Can Underlie Ion Efflux in the Oscillatory Mode of Functioning of Rat Liver Mitochondria

et al. 2022

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Mitochondria are capable of synchronized oscillations in many variables, but the underlying mechanisms are still unclear. In this study, we demonstrated that rat liver mitochondria, when exposed to a pulse of Sr2+ ions in the presence of valinomycin (a potassium ionophore) and cyclosporin A (a specific inhibitor of the permeability transition pore complex) under hypotonia, showed prolonged oscillations in K+ and Sr2+ fluxes, membrane potential, pH, matrix volume, rates of oxygen consumption and H2O2 formation. The dynamic changes in the rate of H2O2 production were in a reciprocal relationship with the respiration rate and in a direct relationship with the mitochondrial membrane potential and other indicators studied. The pre-incubation of mitochondria with Ca2+(Sr2+)-dependent phospholipase A2 inhibitors considerably suppressed the accumulation of free fatty acids, including palmitic and stearic acids, and all spontaneous Sr2+-induced cyclic changes. These data suggest that the mechanism of ion efflux from mitochondria is related to the opening of short-living pores, which can be caused by the formation of complexes between Sr2+(Ca2+) and endogenous long-chain saturated fatty acids (mainly, palmitic acid) that accumulate due to the activation of phospholipase A2 by the ions. A possible role for transient palmitate/Ca2+(Sr2+)-induced pores in the maintenance of ion homeostasis and the prevention of calcium overload in mitochondria under pathophysiological conditions is discussed.

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Section: Discussionmentioning

confidence: 99%

The Short-Term Opening of Cyclosporin A-Independent Palmitate/Sr2+-Induced Pore Can Underlie Ion Efflux in the Oscillatory Mode of Functioning of Rat Liver Mitochondria

et al. 2022

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Transmembrane Transport and Biomimetic Membranes

2023

Physicochemical Mechanics

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Biomimetic Membranes without Proteins but with Aqueous Nanochannels and Facilitated Transport. Minireview

Kocherginsky

2021

Membr. Membr. Technol.

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Imitation of biological membranes and aqueous channels attracts more and more attention. In this review, we mention both the early and very recent papers in this area. Still, we concentrate our attention on less known biomimetic membranes, which are commercial nitrocellulose ultrafilters, impregnated by esters of fatty acids. Pores in filters are filled with lipid-like oils, but aqueous nanochannels are spontaneously formed on the inner pore surfaces with carboxylic groups fixed on nitrocellulose. This combination imitates the most fundamental barrier properties of biological membranes, including specific (per unit thickness) permeability of respiratory gases, transport of nonelectrolytes, water, and ions, cation/anion selectivity, electric impedance, etc. The activation energy for water transport in nanochannels is similar to that in aquaporin. In the presence of fatty acids and other carriers, it is possible to observe facilitated and coupled active counter transport of different metal cations in exchange to H + and without any transmembrane pressure, voltage or ATP. When quinones are dissolved in oils, light-sensitive co-transport of electrons and H + through oil is possible. In this case, redox-active substances are separated by the membrane. They are not mixed, but they still react. Small biomimetic membranes can be used as electrochemical drug sensors in drug detection and screening, medium size membranes—for smart transdermal drug -delivery, and large membranes—for industrial separation and purification. For example, after small modifications, they can be used for metal recovery, including radioactive strontium removal from nuclear waste accumulated and stored since the Cold War.

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Biomimetic Membranes with Aqueous Nanochannels. Phase Transitions and Oscillations

Cited by 3 publications

References 18 publications

The Short-Term Opening of Cyclosporin A-Independent Palmitate/Sr2+-Induced Pore Can Underlie Ion Efflux in the Oscillatory Mode of Functioning of Rat Liver Mitochondria

The Short-Term Opening of Cyclosporin A-Independent Palmitate/Sr2+-Induced Pore Can Underlie Ion Efflux in the Oscillatory Mode of Functioning of Rat Liver Mitochondria

Transmembrane Transport and Biomimetic Membranes

Biomimetic Membranes without Proteins but with Aqueous Nanochannels and Facilitated Transport. Minireview

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