Biomimetic mineralization of chitosan/gelatin cryogels and in vivo biocompatibility assessments for bone tissue engineering

Öfkeli, Fatma; Demir, Didem; Bölgen, Nimet

doi:10.1002/app.50337

Cited by 30 publications

(17 citation statements)

References 43 publications

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“…The SEM results clearly demonstrated that COS incorporation enhanced the bone‐like apatite forming ability of PEGDA scaffolds. This observation is consistent with previous studies reporting the positive effect of chitosan, as the main source of COS, on mineralization 64,65 . The nucleation mechanism of COS can be described as follows.…”

Section: Discussionsupporting

confidence: 92%

“…This observation is consistent with previous studies reporting the positive effect of chitosan, as the main source of COS, on mineralization. 64,65 The nucleation mechanism of COS can be described as follows. The nitrogen atom present in the COS structure is a nucleophile with a special attraction to positive centers due to its two unbound electrons.…”

Section: Discussionmentioning

confidence: 99%

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3D printing of chitooligosaccharide‐polyethylene glycol diacrylate hydrogel inks for bone tissue regeneration

Rajabi

Cabral

Saunderson

et al. 2023

J Biomedical Materials Res

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To date, lack of functional hydrogel inks has limited 3D printing applications in tissue engineering. This study developed a series of photocurable hydrogel inks based on chitooligosaccharide (COS)-polyethylene glycol diacrylate (PEGDA) for extrusionbased 3D printing of bone tissue scaffolds. The scaffolds were prepared by aza-Michael addition of COS and PEGDA followed by photopolymerisation of unreacted PEGDA. The hydrogel inks showed sufficient shear thinning properties required for extrusion 3D printing. The printed scaffolds exhibited excellent shape fidelity and fine microstructure with a resolution of 250 μm. By increasing the COS content, the swelling ratio of the scaffolds decreased, while the compressive strength increased. 3D printed COS-PEGDA scaffolds showed high viability of human bone mesenchymal stem cells in vitro. In addition, scaffolds containing 2 wt% COS showed significantly higher alkaline phosphatase activity, calcium deposition, and bioactivity in simulated body fluid compared to the control (PEGDA). Altogether, 3D printed COS-PEGDA scaffolds represent promising candidates for bone tissue regeneration.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

3D printing of chitooligosaccharide‐polyethylene glycol diacrylate hydrogel inks for bone tissue regeneration

Rajabi

Cabral

Saunderson

et al. 2023

J Biomedical Materials Res

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“…19,[22][23][24] Peng Chen et al 25 prepared hydroxyapatite/gelatin-chitosan composite membrane through wet chemical synthesis, which could be used in tissue engineering. Fatma Öfkeli et al 26 prepared biomimetic CS: Gel composite cryogel scaffolds by SBF soaking method for potential bone tissue engineering applications. Pengfei Ma et al 27 assembled gelatin (Gel), chitosan (CS) and polyvinyl alcohol (PVA) to mimic the extracellular matrix, and added nano-hydroxyapatite to the composite to improve the compressive resistance, adaptability, and surface bioactivity of the composite scaffold.…”

Section: Introductionmentioning

confidence: 99%

Preparation and biological properties of ZnO/hydroxyapatite/chitosan-polyethylene oxide@gelatin biomimetic composite scaffolds for bone tissue engineering

Liu

Feng

et al. 2022

J Biomater Appl

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To imitate the composition of natural bone and further improve the biological property of the materials, ZnO/hydroxyapatite/chitosan-polyethylene oxide@gelatin (ZnO/HAP/CS-PEO@GEL) composite scaffolds were developed. The core-shell structured chitosan-polyethylene oxide@gelatin (CS-PEO@GEL) nanofibers which could form the intramolecular hydrogen bond and achieve an Arg-Gly-Asp (RGD) polymer were first prepared by coaxial electrospinning to mimic the extracellular matrix. To further enhance biological activity, hydroxyapatite (HAP) was grown on the surface of the CS-PEO@GEL nanofibers using chemical deposition and ZnO particles were then evenly distributed on the surface of the above composite materials using RF magnetron sputtering. The SEM results showed that chemical deposition and magnetron sputtering did not destroy the three-dimensional architecture of materials, which was beneficial to cell growth. The cell compatibility and proliferation of MG-63 cells on ZnO/HAP/CS-PEO@GEL composite scaffolds were superior to those on CS-PEO@GEL and HAP/CS-PEO@GEL composite scaffolds. An appropriate amount of ZnO sputtering could promote the adhesion of cells on the composite nanofibers. The structure of bone tissue could be better simulated both in composition and in the microenvironment, which provided a suitable environment for cell growth and promoted the proliferation of MG-63 cells. The biomimetic ZnO/HAP/CS-PEO@GEL composite scaffolds were promising materials for bone tissue engineering.

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“…They described that precipitated pure glycerophosphate shows rough micron-scaled structures, whereas mineral formation by ALP-cleaved glycerophosphate showed nano-scaled structures. Similarly, Öfkeli et al reported gelatin hydrogel biomineralization with simulated body fluid and also observed micron-sized HA-particles in the gels [ 55 ]. We therefore speculate that ALP may play a special role in our system.…”

Section: Discussionmentioning

confidence: 99%

Mineralizing Gelatin Microparticles as Cell Carrier and Drug Delivery System for siRNA for Bone Tissue Engineering

et al. 2022

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The local release of complexed siRNA from biomaterials opens precisely targeted therapeutic options. In this study, complexed siRNA was loaded to gelatin microparticles cross-linked (cGM) with an anhydride-containing oligomer (oPNMA). We aggregated these siRNA-loaded cGM with human mesenchymal stem cells (hMSC) to microtissues and stimulated them with osteogenic supplements. An efficient knockdown of chordin, a BMP-2 antagonist, caused a remarkably increased alkaline phosphatase (ALP) activity in the microtissues. cGM, as a component of microtissues, mineralized in a differentiation medium within 8–9 days, both in the presence and in the absence of cells. In order to investigate the effects of our pre-differentiated and chordin-silenced microtissues on bone homeostasis, we simulated in vivo conditions in an unstimulated co-culture system of hMSC and human peripheral blood mononuclear cells (hPBMC). We found enhanced ALP activity and osteoprotegerin (OPG) secretion in the model system compared to control microtissues. Our results suggest osteoanabolic effects of pre-differentiated and chordin-silenced microtissues.

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Biomimetic mineralization of chitosan/gelatin cryogels and in vivo biocompatibility assessments for bone tissue engineering

Cited by 30 publications

References 43 publications

3D printing of chitooligosaccharide‐polyethylene glycol diacrylate hydrogel inks for bone tissue regeneration

3D printing of chitooligosaccharide‐polyethylene glycol diacrylate hydrogel inks for bone tissue regeneration

Preparation and biological properties of ZnO/hydroxyapatite/chitosan-polyethylene oxide@gelatin biomimetic composite scaffolds for bone tissue engineering

Mineralizing Gelatin Microparticles as Cell Carrier and Drug Delivery System for siRNA for Bone Tissue Engineering

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