Biomimetic mineralization of collagen via an enzyme-aided PILP process

Jee, Sang Soo; Culver, Lauren; Li, Yuping; Douglas, Elliot P.; Gower, Laurie B.

doi:10.1016/j.jcrysgro.2009.11.010

Cited by 63 publications

(86 citation statements)

References 35 publications

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“…The outcomes of PILP remineralization reports have varied by the evaluation methods used, the dimension and the type of collagen scaffolds (Jee, Thula, et al, 2010; Olszta et al, 2007 ; Qi et al, 2012; Thula, Rodriguez, et al, 2011; Thula, Svedlund, et al, 2011). The methods used for evaluating mineral density such as EDS and XCT do not always match mechanical property observations in our dentin cross-sectioned study (Burwell et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

“…The methods used for evaluating mineral density such as EDS and XCT do not always match mechanical property observations in our dentin cross-sectioned study (Burwell et al, 2012). When cross-sections were examined in their reports, specimens from the same biological origin with similar thickness revealed that inner regions were not remineralized or were unclear on defining the extent of remineralization (Jee, Culver, Li, Douglas, & Gower, 2010; Thula, Rodriguez, et al, 2011; Thula, Svedlund, et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

“…Jee et al (Jee, Culver, et al, 2010) have shown penetration of the liquid precursor formed by adding PASP infiltrates at up to 500 μm into turkey tendon, and hypothesized that PILP reaches to the bottom of the lesion based on capillary action, not simply by diffusion. It was proposed that the fluidic character of the precursor phase enables it to be drawn into the spaces in collagen fibrils that are swollen with imbibed water (Jee, Culver, et al, 2010; Olszta et al, 2007). Thula et al reported that 3 mm thickness type I collagen sponge could be remineralized after 6 days whereas solidification occurred and blocked further remineralization of demineralized bone (200~500 μm in thickness) and only surface 100μm was remineralized.…”

Section: Discussionmentioning

confidence: 99%

“…For group 3~6, each block was placed in 40 ml of remineralizing buffer containing Tris, 4.5 mM calcium and 2.1 mM phosphate, at pH 7.4 for 14 days in an incubator at 37°C, with agitation provided by a rocker platform for remineralizing treatment. 100 μg/ ml of Poly-L-aspartic acid (PASP) sodium salt, with molecular weight of 27 kDa (Alamanda polymers, Huntsville, AL) was added to A-PIL and L-PIL groups (Burwell et al, 2012; Jee, Thula, et al, 2010). Negative control groups (A-CAP and L-CAP) were treated in the remineralizing buffer prepared in the same way except without PASP.…”

Section: Methodsmentioning

confidence: 99%

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Recovery after PILP remineralization of dentin lesions created with two cariogenic acids

Saeki

Nonomura

et al. 2017

Archives of Oral Biology

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Objectives Acetate and lactate are important cariogenic acids produced by oral bacteria. They produced different residual dentin structures in artificial lesions of similar depth. We evaluated if such lesions responded in the same way to a polymer-induced-liquid-precursor(PILP) remineralization. Design Dentin blocks obtained from human third molars, divided into 6 groups(n=3). Blocks were demineralized with acetate(66 hours) or lactate(168 hours) buffer at pH 5.0 to create 140 μm target lesion depths. A-DEM and L-DEM groups received no remineralization. Other groups were remineralized for 14 days. 100 μg/mL polyaspartate was added into the remineralizing buffer for A-PIL and L-PIL, whereas A-CAP and L-CAP were treated with the same solution but without polyaspartate. Cross-sectioned blocks were examined for shrinkage and AFM-topography. Line profiles of reduced elastic modulus(Er) were obtained by AFM-based nanoindentation across the lesion. Ultrastructures were examined with TEM. Results A-PIL and L-PIL recovered in shrinkage to the original height of the dentin and it appeared normal with tubules, with increases in Er at both outer flat and inner sloped zones. At the sloped zone, acetate lesions lost more Er but recovery rate after PILP was not statistically different from lactate lesions. A-CAP and L-CAP showed surface precipitates, significantly less recovery in shrinkage or Er as compared to PILP groups. TEM-ultrastructure of PILP groups showed similar structural and mineral components in the sloped zone for lesions produced by either acid. Conclusions The PILP process provided significant recovery of both structure and mechanical properties for artificial lesions produced with acetate or lactate.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Recovery after PILP remineralization of dentin lesions created with two cariogenic acids

Saeki

Nonomura

et al. 2017

Archives of Oral Biology

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“…13 The HA minerals in the bone contribute to better biocompatibility, osteoconductivity and osteointegration. 15,16 Substantial research have been done to deposit HA crystals onto the surface of collagen fibrils (extrafibrillar mineralization) and within collagen fibrils (intrafibrillar mineralization), [17][18][19] and it has been widely accepted that collagen matrix cannot induce intrafibrillar mineralization by itself. Anionic non-collagenous proteins (NCPs) are found to play an important role in modulating HA crystal nucleation and the alignment of apatite deposited within collagen fibrils.…”

Section: Introductionmentioning

confidence: 99%

Development of Biomimetic Scaffolds with Both Intrafibrillar and Extrafibrillar Mineralization

Zhang

Wei

2015

ACS Biomater. Sci. Eng.

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Bone is an organic-inorganic hierarchical biocomposite. Its basic building block is mineralized collagen fibers with both intrafibrillar and extrafibrillar mineralization, which is believed to be regulated by non-collagenous proteins (NCPs) with polyanionic domains. In this study, collagen fibrils with both intrafibrillar and extrafibrillar mineralization were successfully prepared and the mechanism of biomineralization was proposed. Building on this success, a unique biomimetic lamellar scaffold composed of collagen fibrils with both intrafibrillar and extrafibrillar mineralization was fabricated using a combination of self-compression and unidirectional freeze drying approach. To achieve intrafibrillar mineralization, polyacrylic acid (PAA) was used to sequester calcium and phosphate ions to form fluidic PAA-amorphous calcium phosphate (PAA-ACP) nanoprecursors. At the presence of sodium tripolyphosphate (TPP), PAA-ACP nanoprecursors were modulated to orderly deposit within the gap zone of collagen fibrils. The effect of PAA concentration on the intrafibrillar and extrafibrillar mineralization of reconstituted collagen fibrils was investigated. It was found that with the decrease of PAA concentration, the inhibitory effect of PAA on mineralization and the stability of ACP nanoprecursors decreased. As a result, more minerals were deposited both within and on the surface of the collagen fibrils. Moreover, with the ability to reproduce biomineralization of collagen fibrils, it allowed us to fabricate a biomimetic hierarchical collagen/hydroxyapatite scaffolds composed of both intrafibrillar and extrafibrillar minerals using a bottom-up approach. This technique renders a promising biomimetic scaffold, which will be suitable for bone repair and regeneration.

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Complementarity and Uncertainty in Intrafibrillar Mineralization of Collagen

Jiao

Niu

Fan

et al. 2016

Adv Funct Materials

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Biomineralization in vertebrates is a ubiquitous and tightly regulated process which creates hierarchical structures for the skeleton. Because of the lack of understanding and applicability of cell‐based or biological systems to achieve intrafibrillar mineralization, scientists adopted various in vitro methods to elucidate the mechanism of intrafibrillar mineralization. In this article, biomimetic intrafibrillar mineralization of collagen in its wide ramifications is reviewed. It is intriguing how prevailing intrafibrillar mineralization mechanisms derived from two potentially discordant crystallization philosophies were equally adept, depending on the experimental context, at theorizing the formation of calcium phosphate within a fibrillar template. This complementarity is not unique to biomineralization and has precedence in other fundamental physical interpretations. A new intrafibrillar mineralization process based on the use of polycationic process‐directing agent added uncertainty to the use of existing mechanisms in accounting for the observations.

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Biomimetic mineralization of collagen via an enzyme-aided PILP process

Cited by 63 publications

References 35 publications

Recovery after PILP remineralization of dentin lesions created with two cariogenic acids

Recovery after PILP remineralization of dentin lesions created with two cariogenic acids

Development of Biomimetic Scaffolds with Both Intrafibrillar and Extrafibrillar Mineralization

Complementarity and Uncertainty in Intrafibrillar Mineralization of Collagen

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