Biomimetic mineralized hierarchical hybrid scaffolds based on in situ synthesis of nano-hydroxyapatite/chitosan/chondroitin sulfate/hyaluronic acid for bone tissue engineering

Hu, Yimin; Chen, Jingdi; Fan, Tao; Zhang, Yujue; Zhao, Yao; Shi, Xuetao; Zhang, Qiqing

doi:10.1016/j.colsurfb.2017.05.059

Cited by 100 publications

(42 citation statements)

References 55 publications

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“…The binding of GAG and proteins can effectively regulate the release rate of proteins and prevent proteolytic degradation of proteins in tissue fluids [11]. HA is a major component of ECM in many tissues, which plays a crucial role in the development of cartilage and the maintenance of synovial fluid due to its biological properties such as non-inflammatory, biocompatibility and biodegradability [12,13]. Moreover, HA can provide cells with a good cartilage-forming microenvironment that helps maintain the chondrocyte phenotype due to its hydrophilic [14,15].…”

Section: Introductionmentioning

confidence: 99%

“…CS has prominent features including anti-inflammatory activity, inhibition of production of cartilage degradation enzyme and a biological activity at the cellular level, making it play a pivotal role in tissue remodeling. Particularly, CS and HA have property of water retention to cartilage due to their negatively charged structure [12,16].…”

Section: Introductionmentioning

confidence: 99%

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ECM based injectable thermo-sensitive hydrogel on the recovery of injured cartilage induced by osteoarthritis

Cao

Han

et al. 2018

Artificial Cells, Nanomedicine, and Biotechnology

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Intra-articular injection of anti-inflammatory drugs can be a promising strategy for recovery of injured articular cartilage. We prepared a series of injectable thermo-sensitive composite hydrogels, composed of Pluronic F127, glycosaminoglycan (GAG) and bone morphogenetic protein (BMP-2), which was designed to mimic extracellular matrix (ECM). The rheological properties and dissolution rate of composite hydrogels containing chondroitin sulfate or with different hyaluronic acid molecular mass (10k, 90k, 800k) were investigated. Meanwhile, bovine serum albumin (BSA) or FITC-BSA was chosen as model drug loaded into PF/GAG hydrogels to study their sustained release behavior in vitro. The results showed that hydrogels could maintain shapes for more than 16 days and the release rate of BSA in PF/GAG composite gels was much slower than in PF127 gels, due to the affinity between BSA and GAG. Furthermore, increasing the molecular weight of hyaluronic acid correspondingly increased hydrogel dissolution rate and BSA release in the hydrogels. Subsequently, MTT experiments were performed to investigate the toxicity of the hydrogels on mouse pre-osteoblast cell MC3T3-E1. In vivo anti-inflammation results showed that PF/GAG@BMP-2 composite hydrogels had the most efficient efficacy on recovery of injured cartilage, which is induced by osteoarthritis, compared to the control groups (PF127@BMP-2 or BMP-2 saline solution).

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

ECM based injectable thermo-sensitive hydrogel on the recovery of injured cartilage induced by osteoarthritis

Cao

Han

et al. 2018

Artificial Cells, Nanomedicine, and Biotechnology

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“…HA has also been shown to have positive effects on bone formation and cell fate in vivo and in vitro. 14 Despite the increasing development of silica/CS, or HA/ CS hybrids using different approaches, the biocompatibility of these hybrids mainly been explored using in vitro analysis, for example, the viability, proliferation or function detection of different cell clones contacted directly, or indirectly with the constructed hybrids. Thus, for better biological results and user-friendliness, the use of hybrid skill to improve its bioactivities has thus been considered.…”

Section: Introductionmentioning

confidence: 99%

In vivo immuno‐reactivity analysis of the porous three‐dimensional chitosan/SiO₂ and chitosan/SiO₂/hydroxyapatite hybrids

Guo

Dong

Xiao

et al. 2018

J Biomedical Materials Res

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Inorganic/organic hybrid silica-chitosan (CS) scaffolds have promising potential for bone defect repair, due to the controllable mechanical properties, degradation behavior, and scaffold morphology. However, the precise in vivo immuno-reactivity of silica-CS hybrids with various compositions is still poorly defined. In this study, we fabricated the three-dimensional (3D) interconnected porous chitosan-silica (CS/SiO ) and chitosan-silica-hydroxyapatite (CS/SiO /HA) hybrids, through sol-gel process and 3D plotting skill, followed by the naturally or freeze drying separately. Scanning electron microscopy demonstrated the hybrids possessed the uniform geometric structure, while, transmission electron microscopy displayed nanoscale silica, or HA nanoparticles dispersed homogeneously in the CS matrix, or CS/silica hybrids. After intramuscular implantation, CS/SiO and CS/SiO /HA hybrids triggered a local and limited monocyte/macrophage infiltration and myofiber degeneration. Naturally dried CS/SiO hybrid provoked a more severe inflammation than the freeze-dried ones. Dendritic cells were attracted to invade into the implants embedded-muscle, but not be activated to prime the adaptive immunity, because the absence of cytotoxic T cells and B cells in muscle received the implants. Fluorescence-activated cell sorting (FACS) analysis indicated the implanted hybrids were incapable to initiate splenocytes activation. Plasma complement C3 enzyme linked immunosorbent assay (ELISA) assay showed the hybrids induced C3 levels increase in early implanting phase, and the subsequent striking decrease. Thus, the present results suggest that, in vivo, 3D plotted porous CS/SiO and CS/SiO /HA hybrids are relatively biocompatible in vivo, which initiate a localized inflammatory procedure, instead of a systematic immune response. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1223-1235, 2018.

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“…These nHAP composites are reported as bioceramics in the literature and have different applications for their biocompatibility, bioactivity, conductivity, and safe and anti-inflammatory nature. 22 Figure 1 shows the synthesis mechanism of this hybrid scaffold with freeze-drying technology. The homogeneous pore size and well mechanical strength of the composites are due to the use of the nHAP and the interaction between the positive and negative points of CS and HA.…”

mentioning

confidence: 99%

“…Generally, nHAP/CS/CAS/HA composite would be a good potential for the treatment or regeneration of bone scaffolds. 22 Due to a lack of systematic evaluation of HAP and β-tricalcium phosphate (TCP) composite scaffolds for bone repair, their distinctions on bone regeneration have not been clarified yet. Xu et al used HAP and β-TCP to prepare composite scaffolds for bone repair.…”

mentioning

confidence: 99%

A review of using green chemistry methods for biomaterials in tissue engineering

Jahangirian¹,

Lemraski²,

Rafiee-Moghaddam³

et al. 2018

IJN

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Although environmentally safe, or green, technologies have revolutionized other fields (such as consumables, automobiles, etc.), its use in biomaterials is still at its infancy. However, in the few cases in which safe manufacturing technology and materials have been implemented to prevent postpollution and reduce the consumption of synthesized scaffold (such as bone, cartilage, blood cell, nerve, skin, and muscle) has had a significant impact on different applications of tissue engineering. In the present research, we report the use of biological materials as templates for preparing different kinds of tissues and the application of safe green methods in tissue engineering technology. These include green methods for bone and tissue engineering-based biomaterials, which have received the greatest amount of citations in recent years. Thoughts on what is needed for this field to grow are also critically included. In this paper, the impending applications of safe, ecofriendly materials and green methods in tissue engineering have been detailed.

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Biomimetic mineralized hierarchical hybrid scaffolds based on in situ synthesis of nano-hydroxyapatite/chitosan/chondroitin sulfate/hyaluronic acid for bone tissue engineering

Cited by 100 publications

References 55 publications

ECM based injectable thermo-sensitive hydrogel on the recovery of injured cartilage induced by osteoarthritis

ECM based injectable thermo-sensitive hydrogel on the recovery of injured cartilage induced by osteoarthritis

In vivo immuno‐reactivity analysis of the porous three‐dimensional chitosan/SiO₂ and chitosan/SiO₂/hydroxyapatite hybrids

A review of using green chemistry methods for biomaterials in tissue engineering

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Biomimetic mineralized hierarchical hybrid scaffolds based on in situ synthesis of nano-hydroxyapatite/chitosan/chondroitin sulfate/hyaluronic acid for bone tissue engineering

Cited by 100 publications

References 55 publications

ECM based injectable thermo-sensitive hydrogel on the recovery of injured cartilage induced by osteoarthritis

ECM based injectable thermo-sensitive hydrogel on the recovery of injured cartilage induced by osteoarthritis

In vivo immuno‐reactivity analysis of the porous three‐dimensional chitosan/SiO2 and chitosan/SiO2/hydroxyapatite hybrids

A review of using green chemistry methods for biomaterials in tissue engineering

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In vivo immuno‐reactivity analysis of the porous three‐dimensional chitosan/SiO₂ and chitosan/SiO₂/hydroxyapatite hybrids