Biomimetic modified clinical-grade POSS-PCU nanocomposite polymer for bypass graft applications: A preliminary assessment of endothelial cell adhesion and haemocompatibility

Solouk, Atefeh; Cousins, Brian G.; Mirahmadi, Fereshteh; Mirzadeh, Hamid; Nadoushan, M R Jalali; Shokrgozar, Mohammad Ali; Seifalian, Alexander M.

doi:10.1016/j.msec.2014.10.065

Cited by 50 publications

(44 citation statements)

References 41 publications

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“…Chemical incorporation of POSS into the polymer backbone with an ideal dispersion has dramatic enhancements on nanocomposite properties including increases in glass transition temperature (T g ), thermal stability, oxidation resistance, mechanical properties, surface hardness, biocompatibility, wear resistance, as well as reductions in flammability and viscosity [4,7,8].…”

Section: Introductionmentioning

confidence: 99%

In-situ photocrosslinkable nanohybrid elastomer based on polybutadiene/polyhedral oligomeric silsesquioxane

Mirmohammadi

Nekoomanesh‐Haghighi

Gezaz

et al. 2016

Materials Science and Engineering: C

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Hydroxyl functionalized nano-sized POSS or ethyleneglycol as diol monomers was incorporated to hydroxyl-terminated polybutadiene (HTPBD) chain in the presence of fumaryl chloride as extender. Blue light photocrosslinking system based on camphorquinone (photoinitiator) and dimethylaminoethyl methacrylate (accelerator) was applied to cure these two synthesized fumarate based macromers. Self-crosslinkability of unsaturated macromers and also crosslinking in presence of a reactive diluent were investigated in absence and presence of 1,4-butanediol dimethacrylate, respectively. Finally, photocured samples were characterized by XRD, SEM, equilibrium swelling study, TGA, DMTA, AFM and cell culture. The results showed that incorporation of POSS nanoparticle into the polymer matrix with a perfect distribution and dispersion can enhance thermal stability, mechanical and biocompatibility properties which can prove a good potential of this in-situ photocrosslinkable nanohybrid in medical applications.

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Section: Introductionmentioning

confidence: 99%

In-situ photocrosslinkable nanohybrid elastomer based on polybutadiene/polyhedral oligomeric silsesquioxane

Mirmohammadi

Nekoomanesh‐Haghighi

Gezaz

et al. 2016

Materials Science and Engineering: C

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“…This is inherent to the fact that collagen is more hydrophobic than AAc. 4,22 Our data showing that collagen immobilization on AAc-grafted silicone tubes led to moderate wettability is a favorable condition for endothelial cell attachment, because a material surface with either a very high or a very low contact angle is not suitable for cell attachment. [4][5][6] Several reports claimed that carboxyl functional groups create a higher negative surface charge than other commonly used surface functional groups, which causes inhibition of cell attachment.…”

Section: Discussionmentioning

confidence: 91%

“…The integrin family of cell adhesion receptors contains four collagen receptors that are involved in cell-matrix interactions. 22,33 The presence of these receptors implicates that collagen is suitable as a matrix for endothelial cell growth in vitro and might be the reason for increased endothelial cell attachment on AAc SiCol tubes compared with AAc Si tubes. Although AAc Si tubes had toxic effects on endothelial cells in the absence of collagen immobilization, our results also indicate that these tubes can be used as substratum for collagen immobilization and then provide a favorable surface for endothelialization (AAc Si-Col tubes).…”

Section: Discussionmentioning

confidence: 99%

“…22 The solution was gently stirred for 5 h at 4 C to activate the carboxyl groups on AAc Si tubes. Then, AAc Si tubes with activated carboxyl groups were filled with collagen or sodium nitrite-collagen conjugate for collagen immobilization at 4 C for 24 h. Collagenimmobilized AAc-grafted silicone (AAc Si-Col) tubes and sodium nitrite-collagen conjugate immobilized AAc-grafted silicone (AAc Si-Nitrite-Col) tubes were washed in water for 1 min to remove unbound proteins and sodium nitrites, and stored at 4 C. To assess the stability of collagen linked to the tubes, the lumen of AAc Si-Col and AAc Si-Nitrite-Col tubes was washed extensively three times with 5 mL water for 5 min by infusion from one end into the lumen of the tubes using a syringe.…”

Section: Methodsmentioning

confidence: 99%

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Biomimetic modification of silicone tubes using sodium nitrite–collagen immobilization accelerates endothelialization

et al. 2015

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Biomimetic coatings to increase endothelialization of blood-contacting materials in biomedical devices are promising to improve the biocompatibility of these devices. Although a stable extracellular matrix protein coating on a biomaterial's surface is a prerequisite for endothelial cell attachment, it also stimulates platelet adhesion. Therefore, antithrombotic additives, such as nitric oxide donors, to a stable protein coating might lead to successful endothelialization of a material's surface. We aimed to test whether immobilized bioactive nitrite and acidified nitrite-generating sodium nitrite-collagen conjugate on silicone tubes enhances endothelialization by increasing the number of endothelial cells as well as growth hormone production and by decreasing platelet adhesion. Stable collagen immobilization on acrylic acid-grafted silicone tubes decreased the water contact angle from 102° to 56°. Initial 25 µM sodium nitrite in conjugate resulted in maximal growth hormone production (2.5-fold increase) and endothelial cell number (1.8-fold increase) after 2 days. A 95% confluent endothelial cell monolayer on sodium nitrite-collagen conjugate coating was obtained after 6 days. Maximum (2.7-fold) inhibition of platelet adhesion was reached with initial 500 µM sodium nitrite in conjugate. Our data showing that sodium nitrite-collagen conjugate coating with 25-50 µM sodium nitrite on silicone tubes increases the number of endothelial cells attached and inhibits platelet adhesion suggest that this coating is highly promising for use in blood-contacting parts of biomedical devices. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 104B: 1311-1321, 2016.

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“…[41][42][43] Furthermore, organic-inorganic hydrogels containing POSS have also attracted much interest. [41][42][43] Furthermore, organic-inorganic hydrogels containing POSS have also attracted much interest.…”

Section: Introductionmentioning

confidence: 99%

Enhanced mechanical properties and self‐healing behavior of PNIPAM nanocomposite hydrogel by using POSS as a physical crosslinker

Zhang

Shen

Dou

et al. 2019

J of Applied Polymer Sci

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In this study, water-soluble octa(3-chloroammoniumpropyl) silsesquioxane (OCAPS) was found to aggregate into nanoparticles with a positive charge in solution, which can attract persulfate anion radical to initiate N-isopropylacrylamide monomers. Due to the electrostatic and hydrogen bond interaction between OCAPS and poly(N-isopropylacrylamide) (PNIPAM) chain, OCAPS can act as an effective physical crosslinker to result in a nanocomposite hydrogel (OCAPS-PNIPAM) with very small loading N, N 0 -methylene-bisacrylamide (50-100 ppm). The incorporation of OCAPS increases the crosslinking degree of the PNIPAM hydrogel and decreases the swelling ratio in deionized water. The mechanical properties of OCAPS-PNIPAM hydrogel were enhanced greatly by the presence of OCAPS and can be adjusted by the feed ratio. The compression and elasticity moduli vary from 3.52 to 7.59 kPa and 7.67 to 33.91 kPa, respectively. The tensile strength ranges from 6.82 to 243.41 kPa with fracture energy between 503.5 and 4781.7 JÁm −2 . Rheological measurements suggest that OCAPS-PNIPAM hydrogels have stable networks and the loss factor decreases as increasing OCAPS content. OCAPS-PNIPAM hydrogels also can self-heal under certain conditions with low crosslinker loading.

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Biomimetic modified clinical-grade POSS-PCU nanocomposite polymer for bypass graft applications: A preliminary assessment of endothelial cell adhesion and haemocompatibility

Cited by 50 publications

References 41 publications

In-situ photocrosslinkable nanohybrid elastomer based on polybutadiene/polyhedral oligomeric silsesquioxane

In-situ photocrosslinkable nanohybrid elastomer based on polybutadiene/polyhedral oligomeric silsesquioxane

Biomimetic modification of silicone tubes using sodium nitrite–collagen immobilization accelerates endothelialization

Enhanced mechanical properties and self‐healing behavior of PNIPAM nanocomposite hydrogel by using POSS as a physical crosslinker

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