Biomimetic multicomponent polysaccharide/nano-hydroxyapatite composites for bone tissue engineering

Li, Junjie; Sun, Hong; Sun, Da; Yao, Yuli; Yao, Fanglian; Yao, Kangde

doi:10.1016/j.carbpol.2011.04.015

Cited by 97 publications

(53 citation statements)

References 30 publications

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“…It is believed that, this behavior is resulted from Schiff base interactions between the amino groups of chitosan or gelatin and aldehyde groups of glutaraldehyde. [46] In CS-Gel/nHA-PANI composite, it can be seen that the NH bending vibration of chitosan at 1650 cm −1 was shifted to 1670 cm −1 . PANI exhibited the related absorption bands at 1556 cm −1 and 1470 cm −1 , assigned to the stretching vibration of quinoid and benzoid structures, respectively.…”

Section: Characterizationmentioning

confidence: 99%

“…The presence of peak at 21.5°was attributed to the presence of chitosan and gelatin in the scaffold. [46] The CS-Gel/ nHA-PANI scaffold exhibited a semicrystalline nature in comparison to CS-Gel/nHA composite because of introducing less crystalline PANI. Also, the peaks between 25°and 35°, and the peak at 49.56°arised from the existence of the nHA in the CS-Gel/nHA-PANI composite.…”

Section: Characterizationmentioning

confidence: 99%

“…Swelling ability is very important for the absorption of body fluid and transfer of cell nutrients and metabolites. [46] During cell culture, swelling facilitates the cells infiltration into the scaffolds in a three-dimensional fashion. Swelling also increases the pore size and total porosity, and thus maximizing the internal surface area of the scaffolds.…”

Section: Swelling Studiesmentioning

confidence: 99%

“…Also, it assists cell migration and vascularization, all of which are essential factors for new tissue formation. [46] Porosity characterization is based on the presence of open pores which are related to properties such as permeability and surface area of the porous structure. [53] Since a higher density of a scaffold usually leads to higher mechanical strength while a high porosity provides a favorable biological environment, a balance between the porosity and density for a scaffold must be established for the specific application.…”

Section: Porosity and Density Studiesmentioning

confidence: 99%

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Fabrication and characterization of chitosan–gelatin/nanohydroxyapatite–polyaniline composite with potential application in tissue engineering scaffolds

Azhar

Olad

Salehi

2014

Designed Monomers and Polymers

119

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In this study to further improve surface characteristics of tissue engineering scaffolds, a novel composite based on chitosan-gelatin/nanohydroxyapatite-polyaniline (CS-Gel/nHA-PANI) was prepared by blending the synthesized nHA and PANI with chitosan and gelatin solution followed by lyophilization technique. The prepared composite scaffold was characterized using Fourier transform infrared spectroscopy, X-ray diffraction, and scanning electron microscopy techniques. The effects of nHA and PANI on the physicochemical properties of the scaffold including swelling ratio, density, biodegradation, biomineralization, and mechanical behavior were investigated. The composite scaffold was highly porous with the mean pore size of 200 μm. Compared with CS-Gel and CS-Gel/nHA scaffolds, the CS-Gel/nHA-PANI scaffold exhibited lower degradation rate and higher biomineralization and mechanical strength. Preliminary evaluation of the biocompatibility and cytotoxicity of the CS-Gel/nHA-PANI composite scaffold was performed by MTT assay using bovine leukemia virus fetal lamb kidney cell line (BLV-FLK) and then cell attachment studies were assessed using dental pulp stem cells. Results indicated no toxicity, and cells attached and proliferated on the pore surfaces of the scaffold. These findings suggested that the developed scaffold possess the prerequisites and can be used as a scaffold for hard tissues regeneration.

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Section: Characterizationmentioning

confidence: 99%

Section: Characterizationmentioning

confidence: 99%

Section: Swelling Studiesmentioning

confidence: 99%

Section: Porosity and Density Studiesmentioning

confidence: 99%

See 2 more Smart Citations

Fabrication and characterization of chitosan–gelatin/nanohydroxyapatite–polyaniline composite with potential application in tissue engineering scaffolds

Azhar

Olad

Salehi

2014

Designed Monomers and Polymers

119

View full text Add to dashboard Cite

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“…[12][13][14] The addition of nanobioactive ceramic into polymers could not only introduce bioactivity but also improve the mechanical properties of the composites. 15,16 The low solubility of FA is beneficial to improve osseointegration at implant-bone interface, which is considered a promising biomedical material for bone fixation. 17,18 Polyamides (PAs) are the preferred polymer material for orthopedics and dentistry due to their high strength and good biocompatibility.…”

mentioning

confidence: 99%

Development of nanofluorapatite polymer-based composite for bioactive orthopedic implants and prostheses

Hu¹,

Wang²,

Yao³

et al. 2014

IJN

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Fluorapatite with low solubility is a promising biomaterial due to its structure, which is similar to hydroxyapatite. In this study a bioactive composite of nanofluorapatite (n-FA) and polyamide 12 (PA12) was fabricated. The results revealed that the mechanical properties (such as compressive strength and elastic modulus), hydrophilicity, and antibacterial properties of n-FA/PA12 composite were obviously improved by adding n-FA into PA12 as compared with PA12. In addition, cell proliferation of MC3T3-E1 cells cultured on n-FA/PA12 composite was significantly higher than with PA12, and alkaline phosphatase activity of MC3T3-E1 cells on the n-FA/PA12 composite was expressed at obviously higher levels as compared with PA12. The results suggest that n-FA/PA12 composite could support cell proliferation and differentiation, showing good cytocompatibility. Histological evaluation indicates that n-FA/PA12 composite enhances the efficiency of new bone formation with the introduction of n-FA into PA12, and the quantity of the newly formed bone for n-FA/PA12 composite is significantly higher than with PA12. In conclusion, n-FA/PA12 composite exhibits good biocompatibility and osteogenesis, which might be used for various orthopedic prostheses and dental implants.

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Use of Biomineralization Media in Biomimetic Synthesis of Hard Tissue Substitutes

Taş

2014

Ceramic Transactions Series

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Biomimetic multicomponent polysaccharide/nano-hydroxyapatite composites for bone tissue engineering

Cited by 97 publications

References 30 publications

Fabrication and characterization of chitosan–gelatin/nanohydroxyapatite–polyaniline composite with potential application in tissue engineering scaffolds

Fabrication and characterization of chitosan–gelatin/nanohydroxyapatite–polyaniline composite with potential application in tissue engineering scaffolds

Development of nanofluorapatite polymer-based composite for bioactive orthopedic implants and prostheses

Use of Biomineralization Media in Biomimetic Synthesis of Hard Tissue Substitutes

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