Biomimetic Nanomedicine Coupled with Neoadjuvant Chemotherapy to Suppress Breast Cancer Metastasis via Tumor Microenvironment Remodeling

Abstract

The biomimetic enzyme activity of cerium oxide nanoparticles (CeNPs) prefers ultrasmall particle size and bare surface. Unfortunately, those two features are not favorable for its in vivo application due to easy aggregation and fast renal filtration. To take advantage of the activity of CeNP for cancer therapy, a homologous targeted cerium oxide nanoparticle system, targeted CeNP (T-CeNP), with the integration of a biodegradable dendritic mesoporous silica nanoparticle, superoxide dismutase and catalase mimicking CeNPs, and the camouflage coating of cancer cell membrane has been developed. Attributed to the homologous targeting effect of cancer cell membrane, nanoparticles with camouflage coating are retained in the tumor in an orthotopic breast cancer metastatic model. Subsequently, T-CeNP effectively hinders cancer-associated fibroblast transdifferentiation and reprograms it back to a normal fibroblast. Consequently, T-CeNP coupled with doxorubicin reduces the size of primary tumors and prevents the post-surgery lung metastasis and liver metastasis of breast cancer.

Targeting therapy and tumor microenvironment remodeling of triple-negative breast cancer by ginsenoside Rg3 based liposomes

“…Mouse embryonic fibroblast 3T3 is a representative murine normal fibroblast which is sensitive to the cytokine stimulation [47]. Artificial CAFs are usually made in vitro by activating 3T3 cells using TGF-β or CM from tumor cells [47][48][49]. Therefore, to simulate education role of tumor cells on normal fibroblasts-to-CAFs transformation, normal fibroblast 3T3 cells was cultured with tumor CM after different treatment.…”

Biomimetic manganese-eumelanin nanocomposites for combined hyperthermia-immunotherapy against prostate cancer

“…Recently, a variety of therapeutic agents (e.g., monoclonal antibodies, small molecular inhibitors, and complicated nanoparticles) have been extensively explored for M2-to-M1 macrophage repolarization [ 2 , 17 , 19 – 22 ]. Despite promising, these approaches encounter problems such as insufficient immune activation, weak antigen presentation ability, and unsatisfactory antitumor immune response [ 23 , 24 ]. It is highly reasonable to integrate other appropriate synergistic strategies for improved therapeutic efficacy.…”

Phototherapy Using a Fluoroquinolone Antibiotic Drug to Suppress Tumor Migration and Proliferation and to Enhance Apoptosis

“…Scratch assays and plate cell clone formation tests were performed to evaluate the inhibition efficiency of USP on 4T1 cell migration and proliferation. 42 As shown in Figure 4a− d and Figure S13, compared with the control group, the migration and proliferation rates of 4T1 cells that were treated with USP and SPP for 24 h were strongly suppressed in the same intervention, and USP exhibited the largest relative scratch area ratio and stronger inhibition. Transwell assays were used to further evaluate the effect of USP on the invasiveness of 4T1 tumor cells in vitro.…”