Biomimetic PLGA/Strontium-Zinc Nano Hydroxyapatite Composite Scaffolds for Bone Regeneration

Hassan, Mozan Osman; Yuvaraju, Priya; Galiwango, Emmanuel; Rehman, Ihtesham Ur; Al‐Marzouqi, Ali H.; Khaleel, Abbas; Mohsin, Sahar

doi:10.3390/jfb13010013

Cited by 27 publications

(32 citation statements)

References 86 publications

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“…ACP and HA crystals have different calcium (Ca): phosphorus (P) ratio ratios and crystallinity [ 6 ]. Therefore, it is an effective mechanical factor due to its higher stiffness and crystallinity [ 7 , 8 , 9 , 10 ]. HA can effectively promote the growth of osteoblast with high crystallinity.…”

Section: Introductionmentioning

confidence: 99%

Physical and Chemical Characterization of Biomineralized Collagen with Different Microstructures

Niu

Liu

et al. 2022

JFB

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Mineralized collagen is the basic unit in hierarchically organized natural bone with different structures. Polyacrylic acid (PAA) and periodic fluid shear stress (FSS) are the most common chemical and physical means to induce intrafibrillar mineralization. In the present study, non-mineralized collagen, extrafibrillar mineralized (EM) collagen, intrafibrillar mineralized (IM) collagen, and hierarchical intrafibrillar mineralized (HIM) collagen induced by PAA and FSS were prepared, respectively. The physical and chemical properties of these mineralized collagens with different microstructures were systematically investigated afterwards. Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) showed that mineralized collagen with different microstructures was prepared successfully. The pore density of the mineralized collagen scaffold is higher under the action of periodic FSS. Fourier transform infrared spectroscopy (FTIR) analysis showed the formation of the hydroxyapatite (HA) crystal. A significant improvement in the pore density, hydrophilicity, enzymatic stability, and thermal stability of the mineralized collagen indicated that the IM collagen under the action of periodic FSS was beneficial for maintaining collagen activity. HIM collagen fibers, which are prepared under the co-action of periodic FSS and sodium tripolyphosphate (TPP), may pave the way for new bone substitute material applications.

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Section: Introductionmentioning

confidence: 99%

Physical and Chemical Characterization of Biomineralized Collagen with Different Microstructures

Niu

Liu

et al. 2022

JFB

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“…Nanomaterial interfaces lead to an irregular arrangement of atoms, which makes it easy for the atoms to migrate under the external deformation force. Therefore, incorporation of nano ceramic materials improves the mechanical properties of the materials [ 23 , 43 , 44 ]. This could be the reason why PU/HA nano 25% has a lower Young’s modulus than PU.…”

Section: Discussionmentioning

confidence: 99%

Bioactive Composite for Orbital Floor Repair and Regeneration

Alhamoudi

Rehman

Almoshawah

et al. 2022

IJMS

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In the maxillofacial area, specifically the orbital floor, injuries can cause bone deformities in the head and face that are difficult to repair or regenerate. Treatment methodologies include use of polymers, metal, ceramics on their own and in combinations mainly for repair purposes, but little attention has been paid to identify suitable materials for orbital floor regeneration. Polyurethane (PU) and hydroxyapatite (HA) micro- or nano- sized with different percentages (25%, 40% & 60%) were used to fabricate bioactive tissue engineering (TE) scaffolds using solvent casting and particulate leaching methods. Mechanical and physical characterisation of TE scaffolds was investigated by tensile tests and SEM respectively. Chemical and structural properties of PU and PU/HA scaffolds were evaluated by infrared (IR) spectroscopy and Surface properties of the bioactive scaffold were analysed using attenuated total reflectance (ATR) sampling accessory coupled with IR. Cell viability, collagen formed, VEGF protein amount and vascularisation of bioactive TE scaffold were studied. IR characterisation confirmed the integration of HA in composite scaffolds, while ATR confirmed the significant amount of HA present at the top surface of the scaffold, which was a primary objective. The SEM images confirmed the pores’ interconnectivity. Increasing the content of HA up to 40% led to an improvement in mechanical properties, and the incorporation of nano-HA was more promising than that of micro-HA. Cell viability assays (using MG63) confirmed biocompatibility and CAM assays confirmed vascularization, demonstrating that HA enhances vascularization. These properties make the resulting biomaterials very useful for orbital floor repair and regeneration.

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“…In our previously published work [ 59 ], we analyzed the influence of Sr 2+ and Zn 2+ doping on nHAp crystallinity, and we were successfully able to synthesize Sr/Zn-doped nHAp-PLGA composite scaffolds with adequate porosity, bioactivity, and degradability. The XRD pattern and FTIR spectra revealed the phase composition and crystal properties of nHAp in both Sr/Zn-doped powders and composite scaffolds and also confirmed the incorporation of PLGA in the scaffolds.…”

Section: Introductionmentioning

confidence: 99%

Bacterial Inhibition and Osteogenic Potentials of Sr/Zn Co-Doped Nano-Hydroxyapatite-PLGA Composite Scaffold for Bone Tissue Engineering Applications

et al. 2023

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Bacterial infection associated with bone grafts is one of the major challenges that can lead to implant failure. Treatment of these infections is a costly endeavor; therefore, an ideal bone scaffold should merge both biocompatibility and antibacterial activity. Antibiotic-impregnated scaffolds may prevent bacterial colonization but exacerbate the global antibiotic resistance problem. Recent approaches combined scaffolds with metal ions that have antimicrobial properties. In our study, a unique strontium/zinc (Sr/Zn) co-doped nanohydroxyapatite (nHAp) and Poly (lactic-co-glycolic acid) -(PLGA) composite scaffold was fabricated using a chemical precipitation method with different ratios of Sr/Zn ions (1%, 2.5%, and 4%). The scaffolds’ antibacterial activity against Staphylococcus aureus were evaluated by counting bacterial colony-forming unit (CFU) numbers after direct contact with the scaffolds. The results showed a dose-dependent reduction in CFU numbers as the Zn concentration increased, with 4% Zn showing the best antibacterial properties of all the Zn-containing scaffolds. PLGA incorporation in Sr/Zn-nHAp did not affect the Zn antibacterial activity and the 4% Sr/Zn-nHAp-PLGA scaffold showed a 99.7% bacterial growth inhibition. MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) cell viability assay showed that Sr/Zn co-doping supported osteoblast cell proliferation with no apparent cytotoxicity and the highest doping percentage in the 4% Sr/Zn-nHAp-PLGA was found to be ideal for cell growth. In conclusion, these findings demonstrate the potential for a 4% Sr/Zn-nHAp-PLGA scaffold with enhanced antibacterial activity and cytocompatibility as a suitable candidate for bone regeneration.

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Biomimetic PLGA/Strontium-Zinc Nano Hydroxyapatite Composite Scaffolds for Bone Regeneration

Cited by 27 publications

References 86 publications

Physical and Chemical Characterization of Biomineralized Collagen with Different Microstructures

Physical and Chemical Characterization of Biomineralized Collagen with Different Microstructures

Bioactive Composite for Orbital Floor Repair and Regeneration

Bacterial Inhibition and Osteogenic Potentials of Sr/Zn Co-Doped Nano-Hydroxyapatite-PLGA Composite Scaffold for Bone Tissue Engineering Applications

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