Biomimetic polyelectrolyte coating of stem cells suppresses thrombotic activation and enhances its survival and function

Rangasami, Vignesh Kumar; Asawa, Kenta; Teramura, Yuji; Blanc, Katrina Le; Nilsson, Bo; Hilborn, Jöns; Varghese, Oommen P.; Oommen, Oommen P.

doi:10.1016/j.bioadv.2023.213331

Cited by 5 publications

(5 citation statements)

References 46 publications

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“…Again, these findings suggest that heparin benefits MSC proliferation on hydrogels, perhaps as a result of its ability to retain and stabilize growth factors, which halts their deterioration and promotes their interaction with cells. The growth factors with a higher degree of stability facilitate the activation of signaling pathways involved in MSCs proliferation and differentiation. ,, Furthermore, heparin has antiapoptotic and anti-inflammatory properties which promote a more favorable environment for MSC proliferation and it has the ability to bind to cell surface receptors, which can drive cellular alignment along the scaffold’s structure and architecture. , Hence, all of these mechanisms can collectively contribute to an increased proliferation and survival rate of MSCs on the heparinized hydrogels.…”

Section: Resultsmentioning

confidence: 99%

“…34,46,47 Furthermore, heparin has antiapoptotic and antiinflammatory properties which promote a more favorable environment for MSC proliferation and it has the ability to bind to cell surface receptors, which can drive cellular alignment along the scaffold's structure and architecture. 48,49 Hence, all of these mechanisms can collectively contribute to an increased proliferation and survival rate of MSCs on the heparinized hydrogels. Furthermore, it can be seen in Figure 5 that the magnetically stimulated MSCs appeared to align in a preferred direction and are more elongated than the unstimulated MSCs.…”

Section: Effect Of Magnetic Stimulation On Mscsmentioning

confidence: 99%

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Heparinized Acellular Hydrogels for Magnetically Induced Wound Healing Applications

Pires,

Silva,

Ferreira

et al. 2024

ACS Appl. Mater. Interfaces

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The control of angiogenesis has the potential to be used for regulation of several pathological and physiological processes, which can be instrumental on the development of anticancer and wound healing therapeutical approaches. In this study, mesenchymal stem/stromal cells (MSCs) were seeded on magnetic-responsive gelatin, with or without heparin functionalization, and exposed to a static 0.08 T magnetic field (MF), for controlling their anti-inflammatory and angiogenic activity, with the aim of accelerating tissue healing. For the first time, it was examined how the amount of heparin and magnetic nanoparticles (MNPs) distributed on gelatin scaffolds affected the mechanical properties of the hydrogels and the morphology, proliferation, and secretome profiling of MSCs. The findings demonstrated that the addition of MNPs and heparin affects the hydrogel swelling capacity and renders distinct MSC proliferation rates. Additionally, MF acts as a topographical cue to guide MSCs alignment and increases the level of expression of specific genes and proteins that promote angiogenesis. The results also suggested that the presence of higher amounts of heparin (10 μg/cm 3 ) interferes with the secretion and limits the capacity of angiogenic factors to diffuse through the hydrogel and into the culture medium. Ultimately, this study shows that acellular heparinized hydrogels efficiently retain the angiogenic growth factors released by magnetically stimulated MSCs thus rendering superior wound contraction (55.8% ± 0.4%) and cell migration rate (49.4% ± 0.4%), in comparison to nonheparinized hydrogels (35.2% ± 0.7% and 37.8% ± 0.7%, respectively). Therefore, these heparinized magnetic hydrogels can be used to facilitate angiogenesis in various forms of tissue damage including bone defects, skin wounds, and cardiovascular diseases, leading to enhanced tissue regeneration.

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Section: Resultsmentioning

confidence: 99%

Section: Effect Of Magnetic Stimulation On Mscsmentioning

confidence: 99%

Heparinized Acellular Hydrogels for Magnetically Induced Wound Healing Applications

Pires,

Silva,

Ferreira

et al. 2024

ACS Appl. Mater. Interfaces

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“…This process results in the creation of an extracellular coating which protects the cells from physical damage and provides a microenvironment favorable to cell integration and sustained functionality. Importantly, this encapsulation approach preserves cell viability and proliferation without adverse effects such as inflammatory reactions [ 255 , 256 ]. Representative compositions involve the coating of negatively charged islets [ 257 ] with positively charged polymers, such as cationic gelatin [ 256 , 258 , 259 ] and chitosan [ 260 ], followed by subsequent coating with anionic polymers (e.g., alginate, heparin, hyaluronic acid) [ 256 , 258 , 259 , 261 ].…”

Section: Cell Encapsulation Techniquesmentioning

confidence: 99%

“…Importantly, this encapsulation approach preserves cell viability and proliferation without adverse effects such as inflammatory reactions [ 255 , 256 ]. Representative compositions involve the coating of negatively charged islets [ 257 ] with positively charged polymers, such as cationic gelatin [ 256 , 258 , 259 ] and chitosan [ 260 ], followed by subsequent coating with anionic polymers (e.g., alginate, heparin, hyaluronic acid) [ 256 , 258 , 259 , 261 ]. This approach minimizes the required transplant volume for cell transplantation by employing nanometer-sized ultrathin layers (<50 nm) of polymer coating [ 250 ].…”

Section: Cell Encapsulation Techniquesmentioning

confidence: 99%

Recent Advances in Alginate-Based Hydrogels for Cell Transplantation Applications

Kavand,

Noverraz,

Gerber-Lemaire

2024

Pharmaceutics

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With its exceptional biocompatibility, alginate emerged as a highly promising biomaterial for a large range of applications in regenerative medicine. Whether in the form of microparticles, injectable hydrogels, rigid scaffolds, or bioinks, alginate provides a versatile platform for encapsulating cells and fostering an optimal environment to enhance cell viability. This review aims to highlight recent studies utilizing alginate in diverse formulations for cell transplantation, offering insights into its efficacy in treating various diseases and injuries within the field of regenerative medicine.

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“…[10] Silencing specific genes such as tissue factor genes in mesenchymal stromal cells (MSCs) [11] or polyelectrolyte coating of stem cells could prevent thrombotic activation and improve survival after implantation. [12] The use of hydrogels as stem cell carriers has also been reported as a promising approach to overcome these limitations by improving the cell survival and engraftment of the implanted stem cells. [1,13] Nevertheless, a successful injectable and printable hydrogel for stem cell delivery should fulfill all the requirements during formulation, injection, post-injection, and long-term survival phases.…”

Section: Introductionmentioning

confidence: 99%

Fine‐tuning Dynamic Cross–linking for Enhanced 3D Bioprinting of Hyaluronic Acid Hydrogels

Tavakoli,

Krishnan,

Mokhtari

et al. 2023

Adv Funct Materials

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Abstract3D bioprinting of stem cells shows promise for medical applications, but the development of an efficient bioink remains a challenge. Recently, the emergence of dynamically cross–linked hydrogels has advanced this field to obtain self‐healing materials. However, more advanced bioinks are needed that display optimum gelling kinetics, viscoelasticity, shear‐thinning property, structural fidelity, and hold the printed structures sufficiently long enough that allow maturation of the new tissue. Here, a novel extracellular matrix‐based bioink for human mesenchymal stem cells (hMSCs) is presented. Hyaluronic acid (HA) is modified with cysteine and aldehyde functional groups, creating hydrogels with dual cross–linking of disulfide and thiazolidine products. The investigation demonstrates that this cross–linking significantly improves hydrogel stability and biological properties. The bioink exhibits fast gelation kinetics, shear‐thinning, shape‐maintaining properties, high cell survival after printing with >2‐fold increase in stemness marker (OCT3/4 and NANOG), and supports cell proliferation and migration. Disulfide cross–linking contributes to self‐healing and cell migration, while thiazolidine cross–linking reduces gelation time, enhances long‐term stability, and supports cell proliferation. Overall, the HA‐based bioink fulfills the requirements for successful 3D printing of stem cells, providing a promising solution for cell therapy and regenerative medicine.

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Biomimetic polyelectrolyte coating of stem cells suppresses thrombotic activation and enhances its survival and function

Cited by 5 publications

References 46 publications

Heparinized Acellular Hydrogels for Magnetically Induced Wound Healing Applications

Heparinized Acellular Hydrogels for Magnetically Induced Wound Healing Applications

Recent Advances in Alginate-Based Hydrogels for Cell Transplantation Applications

Fine‐tuning Dynamic Cross–linking for Enhanced 3D Bioprinting of Hyaluronic Acid Hydrogels

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