Biomimetic Receptors and Sensors

Dickert, Franz L.

doi:10.3390/s141222525

Cited by 12 publications

(8 citation statements)

References 12 publications

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“…Apart from understanding the complexity of life, high affinity host-guest pairs have tremendous values in both biotechnology and chemical applications. However, the number of synthetic host families that display biomolecular range affinity (K d o 1 mM, K a 4 10 6 M À1 ) and selectivity in aqueous solution is limited to certain cyclophanes, 18 self-assembled receptors, [19][20][21][22][23][24] and cucurbiturils. 1,2 This affinity pair has been exploited in many applications including enzyme-linked immunosorbent assays, protein and nucleic acid detection and purification, and immobilization of biomolecules on surfaces.…”

Section: Introductionmentioning

confidence: 99%

Can we beat the biotin–avidin pair?: cucurbit[7]uril-based ultrahigh affinity host–guest complexes and their applications

et al. 2015

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The design of synthetic, monovalent host-guest molecular recognition pairs is still challenging and of particular interest to inquire into the limits of the affinity that can be achieved with designed systems. In this regard, cucurbit[7]uril (CB[7]), an important member of the host family cucurbit[n]uril (CB[n], n = 5-8, 10, 14), has attracted much attention because of its ability to form ultra-stable complexes with multiple guests. The strong hydrophobic effect between the host cavity and guests, ion-dipole and dipole-dipole interactions of guests with CB portals helps in cooperative and multiple noncovalent interactions that are essential for realizing such strong complexations. These highly selective, strong yet dynamic interactions can be exploited in many applications including affinity chromatography, biomolecule immobilization, protein isolation, biological catalysis, and sensor technologies. In this review, we summarize the progress in the development of high affinity guests for CB[7], factors affecting the stability of complexes, theoretical insights, and the utility of these high affinity pairs in different challenging applications.

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Section: Introductionmentioning

confidence: 99%

Can we beat the biotin–avidin pair?: cucurbit[7]uril-based ultrahigh affinity host–guest complexes and their applications

et al. 2015

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“…The analytical determination of a target molecule is dependent on its specific interaction with a recognition element [ 1 , 2 ]. Thus in sensing technologies, both knowledge on the intermolecular interactions and the ability to control over the recognition processes are keys to improve and enhance the sensitivity and the selectivity of the system [ 3 , 4 ]. Aiming at increasing the sensor performance significant efforts are put on the improvement of the recognition element.…”

Section: Introductionmentioning

confidence: 99%

“…Biological recognition, principally actuated by antibodies and receptors, is known to offer exquisite specificity and selectivity, while often lacking of long term stability, since proteinaceous material exhibits the tendency to unfold when coupled to the transducer. Thus synthetic recognition elements are gaining increasing importance in the field [ 3 , 5 ]. Among the methods for their preparation, the molecular imprinting of polymers (MIP) is a versatile technology for imparting entailed recognition properties to polymeric materials [ 6 – 8 ].…”

Section: Introductionmentioning

confidence: 99%

Surface plasmon resonance based on molecularly imprinted nanoparticles for the picomolar detection of the iron regulating hormone Hepcidin-25

et al. 2015

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BackgroundMolecularly imprinted polymer (MIP) technique is a powerful mean to produce tailor made synthetic recognition sites. Here precipitation polymerization was exploited to produce a library of MIP nanoparticles (NPs) targeting the N terminus of the hormone Hepcidin-25, whose serum levels correlate with iron dis-metabolisms and doping. Biotinylated MIP NPs were immobilized to NeutrAvidin™ SPR sensor chip. The response of the MIP NP sensor to Hepcidin-25 was studied.FindingsMorphological analysis showed MIP NPs of 20–50 nm; MIP NP exhibited high affinity and selectivity for the target analyte: low nanomolar Kds for the interaction NP/Hepcidin-25, but none for the NP/non regulative Hepcidin-20. The MIP NP were integrated as recognition element in SPR allowing the detection of Hepcidin-25 in 3 min. Linearity was observed with the logarithm of Hepcidin-25 concentration in the range 7.2–720 pM. LOD was 5 pM. The response for Hepcidin-20 was limited. Hepcidin-25 determination in real serum samples spiked with known analyte concentrations was also attempted.ConclusionThe integration of MIP NP to SPR allowed the determination of Hepcidin-25 at picomolar concentrations in short times outperforming the actual state of art. Optimization is still needed for real sample measurements in view of future clinical applications.Electronic supplementary materialThe online version of this article (doi:10.1186/s12951-015-0115-3) contains supplementary material, which is available to authorized users.

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“…Some of these ligands include amino acid units [15], sugar molecules [16], diaza-crown moieties [17], and crown ethers containing a pyridine sub-cyclic fragment [18]. In the last few decades, strategies and methods for synthesizing simpler organic compounds that mimic the biological receptors havebeen the prime focus in this research area [19][20][21]. Thus, the artificial receptors having diverse chiral backbones with different elements of chirality [22][23][24][25], incorporating aromatic/heteroaromatic alicyclic rings [26][27][28] in the core structural motif with multiple functionalities [29] have been reported.…”

Section: Introductionmentioning

confidence: 99%

Chiral Heterocycle-Based Receptors for Enantioselective Recognition

et al. 2018

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Abstract:The majority of biomolecules found in living beings are chiral, therefore chiral molecular recognition in living systems is crucial to life. Following Cram's seminal work on the crown-based chiral recognition, prominent research groups have reported innumerable chiral receptors with distinctly different geometrical features and asymmetry elements. Main applications of such chiral receptors are found in chiral chromatography, as for analytical purposes and for bulk separation of racemates.Incorporation of heterocyclic rings in these recognition systems added a new dimension to the existing group of receptors. Heterocycles have additional features such as availability of unshared electron pairs, pronounced conformational features, introduction of hydrogen bonding and presence of permanent dipoles as well as specific spectral properties in certain cases. These features are found to enhance binding properties of the receptors and the selectivity factors between opposite enantiomers, allowing them to be effectively separated. The review presents the synthetic approaches towards these heterocyclic receptors and their distinctly different behavior vis-à-vis carbocyclic receptors.

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Biomimetic Receptors and Sensors

Cited by 12 publications

References 12 publications

Can we beat the biotin–avidin pair?: cucurbit[7]uril-based ultrahigh affinity host–guest complexes and their applications

Can we beat the biotin–avidin pair?: cucurbit[7]uril-based ultrahigh affinity host–guest complexes and their applications

Surface plasmon resonance based on molecularly imprinted nanoparticles for the picomolar detection of the iron regulating hormone Hepcidin-25

Chiral Heterocycle-Based Receptors for Enantioselective Recognition

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