Biomimetic Remineralization with Nano-Hydroxyapatite Treatment of Enamel Erosion (an in vitro study)

Wakwak, Mohamed Ahmed

doi:10.21608/ajdsm.2020.23266.1141

Cited by 4 publications

(2 citation statements)

References 19 publications

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“…Maxillary incisors were acid-etched for one minute with 37% phosphoric acid (Ivoclar) to produce chalky white appearance with white spot lesions (WSLs) that mimic caries, and then rinsed with large amount of deionized water [15].…”

Section: Sample Preparationmentioning

confidence: 99%

In vivo evaluation of electrophoresis-aided re-mineralization on surface topography and chemical composition of de-mineralized enamel: an in vivo animal model study

Ahmed Elmobasher,

Nabih,

Mohamed

2024

jos

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Introduction:The most mineralized and rigid tissue in the human body is tooth enamel. Enamel, the outermost covering of teeth, is made of hydroxyapatite, which is arranged in a well-organized prism structure to absorb loads. De-mineralization occurs when the most widely available and soluble material is removed from the enamel rods' periphery and it is exposed to acids, increasing the enamel's surface porosity, and allowing more acids to diffuse into the tissue and lose mineral ions. Objectives: To evaluate whether an electrophoresis-aided system improved re-mineralization of de-mineralized enamel more than a traditional technique in rabbits with initial enamel lesions using different re-mineralizing agents. Material and methods: Initial enamel lesion was produced through acid etching, and three re-mineralizing agents, such as nano-hydroxyapatite, casein phospho-peptide amorphous calcium phosphate, and bioactive glass, were applied to labial surface using traditional or electrophoresis-aided techniques. Surface topography and chemical composition were evaluated using environmental scanning electron microscope/energy dispersive X-ray spectrometer at baseline, after de-mineralization, and after re-mineralization, at different time periods, such as 2 weeks and 5 weeks for a traditional technique, and 3 hours and 5 hours for an electrophoresis-aided technique. Results: Environmental scanning electron microscope showed that re-mineralizing agents enhanced re-mineralization of surface topography following different application periods. Energy dispersive X-ray spectrometer results revealed that for the traditional technique, there was no statistically significant difference between mean Ca : P ratio values of the three materials at 2 and 5 weeks. For the electrophoresis-aided technique, nanohydroxyapatite showed the highest effect, with the most insignificant effect demonstrated in bioactive glass at both 3 and 5 hours. Conclusions: Applying different re-mineralizing agents significantly influences enamel topography and chemical composition, and accelerate the effect of re-mineralizing agents. Moreover, the dynamics of re-mineralizing agents might be accelerated by electrophoresis.

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Section: Sample Preparationmentioning

confidence: 99%

In vivo evaluation of electrophoresis-aided re-mineralization on surface topography and chemical composition of de-mineralized enamel: an in vivo animal model study

Ahmed Elmobasher,

Nabih,

Mohamed

2024

jos

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“…There are two aspects of treating WSLs; noninvasive technique by remineralization and by minimally invasive technique to improve esthetic by resin in ltration (4) . The latest strategy is treating a non-cavitated caries noninvasively, which is the most conservative approach and preserves the tooth structure through remineralization and reduction of bacterial adhesion to decrease progression of caries that will eventually improve strength, function and esthetics (5) .…”

Section: Introductionmentioning

confidence: 99%

Clinical evaluation of white spot lesions treated by surface pre-reacted glass filler coating material in comparison with Icon resin infiltration by laser fluorescence. (A randomized controlled trial)

Gabr

Maslout

Wakwak

2022

Preprint

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Objective: White spots become one of the initial signs of dental caries. Resin infiltration and remineralization was used to prevent WSLs cavitation. Deposition of minerals depends on the equilibrium between demineralization and remineralization which may happen as a natural repair mechanism. The aim of this study is to compare the effect of PRG Barrier Coat coating material (PRG) against the resin infiltration ICON material in improving the caries lesion state of the WSLs along 1 year. Clinical Considerations: the efficacy of fluoroboro-aluminosilicate Glass surface coating S-PRG fillers and ICON resin infiltration in the ability to improve the aesthetic appearance (masking) and caries lesion state of the WSLs immediately, after 3 months, after 6 months, and after 1 year among adult patients. Laser fluorescence was used to compare the two materials to evaluate the caries progression. PRG Barrier Coat group showed immediate significant improvement in LF scores in demineralization progress and inhibition of caries progression with a significant improved effect. Conclusions: Resin infiltration ICON is more clinical successful treatment for demineralized WSLs. PRG Barrier Coat is useful for short time as control of caries progression and prevention protocol not for treatment of WSLs. Clinical Significance:S-PRG Barrier Coat was clinically successful strategy in inhibition of caries and improve remineralization.

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Effect of Different Desensitizing Protocols on Pulp Inflammatory Responses in Whitened Teeth: A Randomized Clinical Trial

Barros¹,

Mendonça²,

Alencar³

et al. 2023

TODENTJ

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Purpose: This randomized controlled, blind clinical trial evaluated the efficacy of different desensitizing protocols in preventing pulp inflammation after whitening treatment with hydrogen peroxide (HP) at 35% (Whiteness HP 35%). Materials and Methods: Thirty healthy third human molars extracted by orthodontic indication were randomized and allocated into five groups (n=5): NC (negative control): without intervention; PC (positive control): HP; PBM: HP + photobiomodulation with a Watts LASER; CPP: HP + casein phosphopeptide amorphous calcium phosphopeptide (CPP-ACP); and NANO: HP + nano-hydroxyapatite. The in-office whitening was performed in two sessions with a single 45 minutes application at an interval of 48 hours. Pulp tissues were extirpated for immunohistochemical analysis. Immunoreaction for activated caspase-3 was observed, and images were acquired using an Axio Scope A1 microscope. The Kruskal-Wallis test was used to evaluate the immunoexpression of caspase-3. Results: Comparisons between the PC and NC groups revealed a statistically significant difference (p<0.05) for the analysis of caspase-3 immunoexpression. A statistically significant difference (p<0.05) was also observed for the CPP and PBM groups in relation to the PC. Conclusion: Photobiomodulation and CPP-ACP are promising alternatives to minimize pulpal inflammation induced by tooth whitening. Clinical Trial Registration Number: NCT04548674.

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Biomimetic Remineralization with Nano-Hydroxyapatite Treatment of Enamel Erosion (an in vitro study)

Cited by 4 publications

References 19 publications

In vivo evaluation of electrophoresis-aided re-mineralization on surface topography and chemical composition of de-mineralized enamel: an in vivo animal model study

In vivo evaluation of electrophoresis-aided re-mineralization on surface topography and chemical composition of de-mineralized enamel: an in vivo animal model study

Clinical evaluation of white spot lesions treated by surface pre-reacted glass filler coating material in comparison with Icon resin infiltration by laser fluorescence. (A randomized controlled trial)

Effect of Different Desensitizing Protocols on Pulp Inflammatory Responses in Whitened Teeth: A Randomized Clinical Trial

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