Biomimetic scaffolds with three-dimensional undulated microtopographies

Yu, Jonelle Z.; Korkmaz, Emrullah; Berg, Monica I.; LeDuc, Philip R.; Özdoğanlar, O. Burak

doi:10.1016/j.biomaterials.2017.02.014

Cited by 35 publications

(15 citation statements)

References 49 publications

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“…[ 7 ] Also, mechanical micromilling was used to fabricate microfeatures on a blank PMMA workpiece (McMaster Carr, IL, USA) using tungsten carbide micro end mills. [ 73 ] The microtopology geometries were created in SolidWorks 2016 (Dassault Systèmes, Vélizy‐Villacoublay, France) and tool paths were generated using Master CAM X7 software in g‐code language. In addition, 3D printing was used to fabricate multistep low resolution master mold geometries through stereolithography.…”

Section: Methodsmentioning

confidence: 99%

Polycarbonate Heat Molding for Soft Lithography

Sönmez

Coyle

Taylor

et al. 2020

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wafers. This type of fabrication requires user expertise to be successful as most of the steps are manual, are associated with high material and equipment costs, demand specialized facilities that may not be available to everyone, and are challenging for mass fabrication. This creates a barrier for the utilization and adaptation of this technology especially for nonexperts and also can cause issues for the commercialization of the products fabricated through this approach. [5] Furthermore, soft lithography has also been challenging because of problems related to the properties of the master molds. The composite nature of Si-Pr master molds results in limited casting lifetime. The differences between the thermal expansion coefficient of the photoresist and the silicon layers result in thermal stress and consequent delamination after a number of heating-cooling cycles. [6] This issue becomes more prominent when thicker photoresist layers with high-aspect-ratio features are present in the master mold. Fabrication of Si-Pr master molds with highaspect-ratio features also poses additional problems such as the repeatability of the uniformity of spin-coating thickness, challenges associated with developing high-aspect ratio indentations (e.g., trenches), and poor adhesion between crosslinked photoresist and silicon wafers. Furthermore, damage can occur in high-aspect ratio features during the manual peeling of the cured PDMS from the mold, which is frequently experienced during the PDMS replica molding process. Such issues render Si-Pr master molds fragile and not particularly robust.To overcome the issues associated with Si-Pr master molds, several other master mold fabrication techniques have been used including mechanical micromilling and 3D printing. Mechanical micromilling allows for fabrication of high-resolution master molds on a diversity of substrates automatically through computer-aided manufacturing (CAM) software and automated stages. [7] However, mechanical micromilling also requires sophisticated tools and expensive equipment, cannot be scaled up for mass production easily, and fabrication parameters are needed to be optimized for each substrate type and geometry. Also, the aspect ratio of the trenches and microwells are limited due to micro end mill failure [8] along with the inability of the milling process to produce sharp inner corners. [7] Another approach is 3D printing in the additive manufacturing domain, which has been improving its ability to create more complex master mold geometries with automation in shorter time periods. [9,10] However, this approach is limited in its feature Soft lithography enables rapid microfabrication of many types of micro systems by replica molding elastomers into master molds. However, master molds can be very costly, hard to fabricate, vulnerable to damage, and have limited casting life. Here, an approach for the multiplication of master molds into monolithic thermoplastic sheets for further soft lithographic fabrication is introduced. The technique is tested with ma...

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Section: Methodsmentioning

confidence: 99%

Polycarbonate Heat Molding for Soft Lithography

Sönmez

Coyle

Taylor

et al. 2020

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“… 170 Gelatin-based hydrogels with microsized undulation topography by casting method was shown to be suitable for cells to grow in the shape pf undulation and formed multiple monolayers to resemble skin. 30 Non-adhesive hydrogels with programmable geometries have also shown the capability to control self-organization of cellular aggregates. 171 In addition, chitosan hydrogels with microwells prepared with molding processes facilitated the co-culture of hepatocyte spheroids and fibroblast monolayers, enabling the study of heterotypic cell–cell interaction.…”

Section: Surface Topography Affect Cell Responses On Hydrogelmentioning

confidence: 99%

The effects of surface topography modification on hydrogel properties

2021

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Hydrogel has been an attractive biomaterial for tissue engineering, drug delivery, wound healing, and contact lens materials, due to its outstanding properties, including high water content, transparency, biocompatibility, tissue mechanical matching, and low toxicity. As hydrogel commonly possesses high surface hydrophilicity, chemical modifications have been applied to achieve the optimal surface properties to improve the performance of hydrogels for specific applications. Ideally, the effects of surface modifications would be stable, and the modification would not affect the inherent hydrogel properties. In recent years, a new type of surface modification has been discovered to be able to alter hydrogel properties by physically patterning the hydrogel surfaces with topographies. Such physical patterning methods can also affect hydrogel surface chemical properties, such as protein adsorption, microbial adhesion, and cell response. This review will first summarize the works on developing hydrogel surface patterning methods. The influence of surface topography on interfacial energy and the subsequent effects on protein adsorption, microbial, and cell interactions with patterned hydrogel, with specific examples in biomedical applications, will be discussed. Finally, current problems and future challenges on topographical modification of hydrogels will also be discussed.

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“…Latest advances in skin 3D scaffolds include the use of novel composite biomaterials such as a gelatine‐sulfonated silk composite scaffold, which is used to stimulate neovascularization within the construct, and the applications of innovative technologies in scaffold fabrication such as stereolithographic 3D bioprinting, a layer‐by‐layer photopolymerization system which provides good material selection, resolution, speed and low surface roughness . Another example is the use of mechanical micromilling technology, a precise fabrication of undulated microtopographies of poly(methyl methacrylate) to mimic dermal papillae of skin . These innovative technologies allow to have the possibility to overcome one of the main problems in skin TE which is the deficiency of dermal vascularization, as well as improving the resolution and biomimicry of the scaffolds.…”

Section: Current and Future Clinical Applicationsmentioning

confidence: 99%

“…91,92 Another example is the use of mechanical micromilling technology, a precise fabrication of undulated microtopographies of poly(methyl methacrylate) to mimic dermal papillae of skin. 93 These innovative technologies allow to have the possibility to overcome one of the main problems in skin TE which is the deficiency of dermal vascularization, as well as improving the resolution and biomimicry of the scaffolds.…”

Section: Current and Future Clinical Applicationsmentioning

confidence: 99%

Therapeutic strategies for skin regeneration based on biomedical substitutes

Chocarro‐Wrona

López‐Ruiz

Perán

et al. 2019

Acad Dermatol Venereol

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Regenerative medicine and tissue engineering (TE) have experienced significant advances in the development of in vitro engineered skin substitutes, either for replacement of lost tissue in skin injuries or for the generation of in vitro human skin models to research. However, currently available skin substitutes present different limitations such as expensive costs, abnormal skin microstructure and engraftment failure. Given these limitations, new technologies, based on advanced therapies and regenerative medicine, have been applied to develop skin substitutes with several pharmaceutical applications that include injectable cell suspensions, cell‐spray devices, sheets or 3Dscaffolds for skin tissue regeneration and others. Clinical practice for skin injuries has evolved to incorporate these innovative applications to facilitate wound healing, improve the barrier function of the skin, prevent infections, manage pain and even to ameliorate long‐term aesthetic results. In this article, we review current commercially available skin substitutes for clinical use, as well as the latest advances in biomedical and pharmaceutical applications used to design advanced therapies and medical products for wound healing and skin regeneration. We highlight the current progress in clinical trials for wound healing as well as the new technologies that are being developed and hold the potential to generate skin substitutes such as 3D bioprinting‐based strategies.

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Biomimetic scaffolds with three-dimensional undulated microtopographies

Cited by 35 publications

References 49 publications

Polycarbonate Heat Molding for Soft Lithography

Polycarbonate Heat Molding for Soft Lithography

The effects of surface topography modification on hydrogel properties

Therapeutic strategies for skin regeneration based on biomedical substitutes

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