Biomimetic Sheath Membrane via Electrospinning for Antiadhesion of Repaired Tendon

Abstract

The hierarchical architecture and complex biologic functions of native sheath make its biomimetic substitute a daunting challenge. In this study, a biomimetic bilayer sheath membrane consisting of hyaluronic acid-loaded poly(ε-caprolactone) (HA/PCL) fibrous membrane as the inner layer and PCL fibrous membrane as the outer layer was fabricated by a combination of sequential and microgel electrospinning technologies. This material was characterized by mechanical testing and analysis of morphology, surface wettability, and drug release. Results of an in vitro drug release study showed sustained release. The outer layer had fewer cells proliferating on its surface compared to tissue culture plates or the inner layer. In a chicken model, peritendinous adhesions were reduced and tendon gliding were improved by the application of this sheath membrane. Taken together, our results demonstrate that such a biomimetic bilayer sheath can release HA sustainably as well as promoting tendon gliding and preventing adhesion.

The past, present and future in scaffold-based tendon treatments

“…Electro-spun poly-ε-caprolactone (PCL) scaffolds coated with tendon-derived ECM promoted ADSC attachment, homogeneous distribution within the scaffolds and expression of tendon specific markers [422]. Functionalisation using HA has enabled integration of PCL electro-spun scaffolds into a chicken foot tendon model, whilst preventing postsurgical adhesions [423].…”

Dual functional core–sheath electrospun hyaluronic acid/polycaprolactone nanofibrous membranes embedded with silver nanoparticles for prevention of peritendinous adhesion

“…Through a sequential electrospinning technique, a bi-layer NFM with HA and Ag nanoparticles was prepared. In this study, an HA microgel was loaded into the PCL solution to prepare the inner membrane layer, while the outer layer was prepared from a pristine PCL solution [19]. A co-axial electrospinning process could produce multiple fibrous components, called coresheath fibers, in a single step, which can accommodate both hydrophilic and hydrophobic polymers simultaneously [20].…”

Modular and Versatile Spatial Functionalization of Tissue Engineering Scaffolds through Fiber‐Initiated Controlled Radical Polymerization

“…A porous structure manufactured with a processing technique that can add to this versatility is required; this can be achieved with electrospinning. Electrospinning is an ideal technique for producing 3D networks of fibers of tunable size, orientation, composition, and density that mimic the properties of native extracellular matrix (ECM)1, 2, 3 and can generate scaffolds with spatially arranged functionalization through layering of various polymers during electrospinning 4, 5, 6, 7, 8, 9. Developing a controlled method that allows multiple zonally arranged functional groups within a continuous scaffold allows for the production of a hierarchical structure that can modulate cell behavior within each functional zone.…”