Biomimetic soft fibrous hydrogels for contractile and pharmacologically responsive smooth muscle

Ding, Yonghui; Xu, Xin; Sharma, Sadhana; Floren, Michael; Stenmark, Kurt R.; Bryant, Stephanie J.; Neu, Corey P.; Tan, Wei

doi:10.1016/j.actbio.2018.05.015

Cited by 30 publications

(23 citation statements)

References 44 publications

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“…The fibrous hydrogel system showed different biomimetic smooth muscle properties like modifiable structures, composition and mechanical stiffness. With the help of protein array technology and the hydrogels, they demonstrated the in vitro pharmacological responsiveness and contractility of the smooth muscles [82].…”

Section: Thiol-ene-based Click Hydrogels For Tissue Engineeringmentioning

confidence: 99%

Click Chemistry-Based Injectable Hydrogels and Bioprinting Inks for Tissue Engineering Applications

Janarthanan

Noh

2018

Tissue Eng Regen Med

120

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BACKGROUND: The tissue engineering and regenerative medicine approach require biomaterials which are biocompatible, easily reproducible in less time, biodegradable and should be able to generate complex three-dimensional (3D) structures to mimic the native tissue structures. Click chemistry offers the much-needed multifunctional hydrogel materials which are interesting biomaterials for the tissue engineering and bioprinting inks applications owing to their excellent ability to form hydrogels with printability instantly and to retain the live cells in their 3D network without losing the mechanical integrity even under swollen state. METHODS: In this review, we present the recent developments of in situ hydrogel in the field of click chemistry reported for the tissue engineering and 3D bioinks applications, by mainly covering the diverse types of click chemistry methods such as Diels-Alder reaction, strain-promoted azide-alkyne cycloaddition reactions, thiol-ene reactions, oxime reactions and other interrelated reactions, excluding enzyme-based reactions. RESULTS: The click chemistry-based hydrogels are formed spontaneously on mixing of reactive compounds and can encapsulate live cells with high viability for a long time. The recent works reported by combining the advantages of click chemistry and 3D bioprinting technology have shown to produce 3D tissue constructs with high resolution using biocompatible hydrogels as bioinks and in situ injectable forms. CONCLUSION: Interestingly, the emergence of click chemistry reactions in bioink synthesis for 3D bioprinting have shown the massive potential of these reaction methods in creating 3D tissue constructs. However, the limitations and challenges involved in the click chemistry reactions should be analyzed and bettered to be applied to tissue engineering and 3D bioinks. The future scope of these materials is promising, including their applications in in situ 3D bioprinting for tissue or organ regeneration.

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Section: Thiol-ene-based Click Hydrogels For Tissue Engineeringmentioning

confidence: 99%

Click Chemistry-Based Injectable Hydrogels and Bioprinting Inks for Tissue Engineering Applications

Janarthanan

Noh

2018

Tissue Eng Regen Med

120

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“…gelatin methacrylate (GelMA) [27][28][29] emerged. Recently, the combination of hydrogels and fibrous scaffold has been used to engineer fiberreinforced hydrogels 30,31 applied in the regeneration of soft tissues, including cartilage [32][33][34][35][36] , heart valve 23,37,38 , tendon 39 , and muscle 40 . The fibrous structure of the fiber hydrogel construct can resemble and simulate the biological fibers of the native soft tissues and supply the hierarchical and morphological cues to induce the specific growth and differentiation of cells.…”

mentioning

confidence: 99%

Fiber reinforced GelMA hydrogel to induce the regeneration of corneal stroma

Kong

Chen²,

Li³

et al. 2020

Nat Commun

228

190

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Regeneration of corneal stroma has always been a challenge due to its sophisticated structure and keratocyte-fibroblast transformation. In this study, we fabricate grid poly (ε-caprolactone)-poly (ethylene glycol) microfibrous scaffold and infuse the scaffold with gelatin methacrylate (GelMA) hydrogel to obtain a 3 D fiber hydrogel construct; the fiber spacing is adjusted to fabricate optimal construct that simulates the stromal structure with properties most similar to the native cornea. The topological structure (3 D fiber hydrogel, 3 D GelMA hydrogel, and 2 D culture dish) and chemical factors (serum, ascorbic acid, insulin, and β-FGF) are examined to study their effects on the differentiation of limbal stromal stem cells to keratocytes or fibroblasts and the phenotype maintenance, in vitro and in vivo tissue regeneration. The results demonstrate that fiber hydrogel and serum-free media synergize to provide an optimal environment for the maintenance of keratocyte phenotype and the regeneration of damaged corneal stroma.

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“…Fibers composed of synthetic polymers, like polycaprolactone, which contain semi‐crystalline and hydrophobic domains remain rigid with limited water absorption when placed in aqueous environments. In recent years, electrospinning been applied to create fibrous hydrogels 13–16 . This process typically requires an initiator and/or crosslinking agent to be added to the precursor solution and then a second step is required after electrospinning to induce polymerization and crosslinking 14,15 .…”

Section: Introductionmentioning

confidence: 99%

The effects of processing variables on electrospun poly(ethylene glycol) fibrous hydrogels formed from the thiol‐norbornene click reaction

Sharma

Monteleone

Kopyeva

et al. 2021

J of Applied Polymer Sci

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Electrospinning has been used to create scaffolds with tunable micro/nano architecture, stiffness, and porosity to mimic native extracellular matrix. This study investigated the effects of electrospinning parameters and hydrogel formulation (solvent and crosslinker type) on the architecture and properties of fibrous poly(ethylene glycol) (PEG) hydrogels formed from a photoclick thiolnorbornene reaction. Fibrous hydrogels were prepared using hydrogel precursors (four-arm PEG norbornene and multi-thiol crosslinker), sacrificial poly(ethylene oxide) (PEO, 400 kDa), and photoinitiator (I2959) in either 2,2-triflouroethanol (TFE) or water. Three thiol crosslinkers-2,2 0 -(ethylenedioxy)diethanethiol (EDT), pentaerythritol tetrakis(3mercaptopropionate) (PTMP), and PEG dithiol (PEGDT)-were investigated. Fibrous PEG networks with uniform fibers were produced at applied voltages of 10 or 12 kV for TFE and 16 kV for water. Fiber diameters of electrospun hydrogels were largely affected by the solvent when combined with PEO concentration and ranged from 0.5 to 3.5 mm in dry state. While the effect of crosslinker type on fiber diameter, morphology, and porosity of the fibrous hydrogel was minimal, it did modulate its shear modulus. To this end, this study provides the groundwork for selecting processing parameters to achieve desired properties of fibrous PEG thiol-norbornene hydrogels for intended tissue engineering applications ranging from neural, cardiovascular to musculoskeletal.

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Biomimetic soft fibrous hydrogels for contractile and pharmacologically responsive smooth muscle

Cited by 30 publications

References 44 publications

Click Chemistry-Based Injectable Hydrogels and Bioprinting Inks for Tissue Engineering Applications

Click Chemistry-Based Injectable Hydrogels and Bioprinting Inks for Tissue Engineering Applications

Fiber reinforced GelMA hydrogel to induce the regeneration of corneal stroma

The effects of processing variables on electrospun poly(ethylene glycol) fibrous hydrogels formed from the thiol‐norbornene click reaction

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