Biomimetic Topography: Bioinspired Cell Culture Substrates and Scaffolds

Wang, Lin

doi:10.5772/14383

Cited by 25 publications

(24 citation statements)

References 83 publications

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“…Copyright, 2010)be a strong enabler of adhesion if the surface forces are attractive. Similar adhesive properties have been observed in other fields including osteointegration where it is believed that implant binding strength dramatically improves with hierarchical surface topographyWang and Carrier 2011).…”

supporting

confidence: 75%

Hybrid and Hierarchical Composite Materials

Kim¹,

Randow²,

Sano³

2015

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supporting

confidence: 75%

Hybrid and Hierarchical Composite Materials

Kim¹,

Randow²,

Sano³

2015

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“…Specifically, scaffolds with aligned fiber configuration (loaded with 100 ng of bFGF) had a 28% increase in sprout length in comparison to scaffolds with random fiber configuration (loaded with 100 ng of bFGF) ( p = 0.0012). This could be attributed to the differences on the swelling profiles between the aligned and random scaffolds, which in turn could influence cytokine release profiles [48–51]. In fact when we determined the swelling profiles of aligned and random scaffolds, there was a 13% increase in the rate of swelling for aligned versus random (Fig.…”

Section: Resultsmentioning

confidence: 99%

bFGF-containing electrospun gelatin scaffolds with controlled nano-architectural features for directed angiogenesis

et al. 2012

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Current therapeutic angiogenesis strategies are focused on the development of biologically responsive scaffolds that can deliver multiple angiogenic cytokines and/or cells in ischemic regions. Herein, we report on a novel electrospinning approach to fabricate cytokine-containing nanofibrous scaffolds with tunable architecture to promote angiogenesis. Fiber diameter and uniformity were controlled by varying the concentration of the polymeric (i.e. gelatin) solution, the feed rate, needle to collector distance, and electric field potential between the collector plate and injection needle. Scaffold fiber orientation (random vs. aligned) was achieved by alternating the polarity of two parallel electrodes placed on the collector plate thus dictating fiber deposition patterns. Basic fibroblast growth factor (bFGF) was physically immobilized within the gelatin scaffolds at variable concentrations and human umbilical vein endothelial cells (HUVEC) were seeded on the top of the scaffolds. Cell proliferation and migration was assessed as a function of growth factor loading and scaffold architecture. HUVECs successfully adhered onto gelatin B scaffolds and cell proliferation was directly proportional to the loading concentrations of the growth factor (0–100 bFGF ng/mL). Fiber orientation had a pronounced effect on cell morphology and orientation. Cells were spread along the fibers of the electrospun scaffolds with the aligned orientation and developed a spindle-like morphology parallel to the scaffold's fibers. In contrast, cells seeded onto the scaffolds with random fiber orientation, did not demonstrate any directionality and appeared to have a rounder shape. Capillary formation (i.e. sprouts length and number of sprouts per bead), assessed in a 3-D in vitro angiogenesis assay, was a function of bFGF loading concentration (0 ng, 50 ng and 100 ng per scaffold) for both types of electrospun scaffolds (i.e. with aligned or random fiber orientation).

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“…In addition, the size, density, and distribution of the topographical features found on the surface of the ECM are also dependent upon the types of cellular tissues that form the ECM [32, 133]. Therefore, it is important that the information derived from the specific cell generated ECM topography is translated into an effective fabrication process that replicates these surface features onto tissue culture substrates and scaffolds [134]. …”

Section: Construction Of a Tissue Scaffoldmentioning

confidence: 99%

Engineering a Biocompatible Scaffold with Either Micrometre or Nanometre Scale Surface Topography for Promoting Protein Adsorption and Cellular Response

Poinern

Ali

et al. 2013

International Journal of Biomaterials

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Surface topographical features on biomaterials, both at the submicrometre and nanometre scales, are known to influence the physicochemical interactions between biological processes involving proteins and cells. The nanometre-structured surface features tend to resemble the extracellular matrix, the natural environment in which cells live, communicate, and work together. It is believed that by engineering a well-defined nanometre scale surface topography, it should be possible to induce appropriate surface signals that can be used to manipulate cell function in a similar manner to the extracellular matrix. Therefore, there is a need to investigate, understand, and ultimately have the ability to produce tailor-made nanometre scale surface topographies with suitable surface chemistry to promote favourable biological interactions similar to those of the extracellular matrix. Recent advances in nanoscience and nanotechnology have produced many new nanomaterials and numerous manufacturing techniques that have the potential to significantly improve several fields such as biological sensing, cell culture technology, surgical implants, and medical devices. For these fields to progress, there is a definite need to develop a detailed understanding of the interaction between biological systems and fabricated surface structures at both the micrometre and nanometre scales.

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Biomimetic Topography: Bioinspired Cell Culture Substrates and Scaffolds

Cited by 25 publications

References 83 publications

Hybrid and Hierarchical Composite Materials

Hybrid and Hierarchical Composite Materials

bFGF-containing electrospun gelatin scaffolds with controlled nano-architectural features for directed angiogenesis

Engineering a Biocompatible Scaffold with Either Micrometre or Nanometre Scale Surface Topography for Promoting Protein Adsorption and Cellular Response

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