Biomimetic versus sintered macroporous calcium phosphate scaffolds enhanced bone regeneration and human mesenchymal stromal cell engraftment in calvarial defects

Brennan, Meadhbh Á.; Monahan, David S.; Brulin, Bénédicte; Gallinetti, Sara; Humbert, P.; Tringides, Christina M.; Canal, Cristina; Ginebra, Maria Pau; Layrolle, Pierre

doi:10.1016/j.actbio.2021.09.007

Cited by 22 publications

(18 citation statements)

References 76 publications

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“…On their own, they cannot facilitate sufficient bone formation. Indeed, after 8 weeks, the range has hitherto been 5–60%, 20 , 93 − 98 and for cell-free scaffolds, 2–42% after 8 weeks, 99 − 107 respectively. In this study, we have tried to overcome these limitations by using a fairly new approach in the field compensating for both the soft nature of the bone ECM and the hard mineral-like properties of mineralized bone.…”

Section: Discussionmentioning

confidence: 99%

Multi-leveled Nanosilicate Implants Can Facilitate Near-Perfect Bone Healing

Keshavarz

Alizadeh

Kadumudi

et al. 2023

ACS Appl. Mater. Interfaces

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Several studies have shown that nanosilicate-reinforced scaffolds are suitable for bone regeneration. However, hydrogels are inherently too soft for load-bearing bone defects of critical sizes, and hard scaffolds typically do not provide a suitable three-dimensional (3D) microenvironment for cells to thrive, grow, and differentiate naturally. In this study, we bypass these long-standing challenges by fabricating a cell-free multi-level implant consisting of a porous and hard bone-like framework capable of providing load-bearing support and a softer native-like phase that has been reinforced with nanosilicates. The system was tested with rat bone marrow mesenchymal stem cells in vitro and as a cell-free system in a critical-sized rat bone defect. Overall, our combinatorial and multi-level implant design displayed remarkable osteoconductivity in vitro without differentiation factors, expressing significant levels of osteogenic markers compared to unmodified groups. Moreover, after 8 weeks of implantation, histological and immunohistochemical assays indicated that the cell-free scaffolds enhanced bone repair up to approximately 84% following a near-complete defect healing. Overall, our results suggest that the proposed nanosilicate bioceramic implant could herald a new age in the field of orthopedics.

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Section: Discussionmentioning

confidence: 99%

Multi-leveled Nanosilicate Implants Can Facilitate Near-Perfect Bone Healing

Keshavarz

Alizadeh

Kadumudi

et al. 2023

ACS Appl. Mater. Interfaces

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“…Specifically, amorphous calcium phosphate (ACP) coatings are biologically relevant since they foster new bone formation by osteoblasts, inducing faster mineralization and osteogenic induction (Vaquette et al, 2013). ACP also enhances MSC-mediated reconstruction of bone defects and engraftment (Brennan et al, 2021), and increases titanium implant biocompatibility and bonding strength, even in the presence of coating resorption (Maxian et al, 1993).…”

Section: Discussionmentioning

confidence: 99%

Amorphous calcium phosphate-coated surfaces as a model for bone microenvironment in prostate cancer

Martin

Das

Xue

et al. 2023

Preprint

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Bone metastasis remains one of the biggest challenges in the treatment of prostate cancer, and other solid tumors such as breast, lung, and colon. Modeling a complex microenvironment in-vitro, such as the bone niche, requires interrogation of cell-cell interactions, specific extracellular matrix proteins and a high calcium environment. Here, we present a fast and cost-effective system in which commercially available, non-adhesive, cell culture vessels are coated with amorphous calcium phosphate (ACP) as a surrogate for bone matrix. We further present modified protocols for subculturing cells, as well as nucleic acid and protein collection in high calcium samples. We find that prostate epithelial cell lines show increased adhesion and proliferation when cultured in these surfaces, as well as independence from androgen starvation. We observe gene expression changes on ACP surfaces in early adenocarcinoma cell lines which may reflect alterations relevant to prostate cancer progression.

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“…Although a few studies have explored the regenerative potential of terminally differentiated cells, e.g., osteoblasts [63,64], most research on cranial bone healing has been conducted with adult stem cells, mainly adipose-and bone marrow-derived stem cells, but also with muscle-, periodontal ligament-, neural crest-, cranial suture-and human dental pulp-derived stem cells, among others [65][66][67][68][69][70][71][72][73][74][75][76][77][78][79]. Collectively, most studies have reported the superior ability of adult stem cells to heal critical size calvarial defects compared with implantation of carriers or scaffolds lacking cells [65,66,73,75,[77][78][79]. A study conducted on rabbit calvaria showed that the regeneration ability of bone marrow-derived stem cells is similar to that achieved by autologous bone [80].…”

Section: Research On Cell-based Strategiesmentioning

confidence: 99%

A Narrative Review of Cell-Based Approaches for Cranial Bone Regeneration

et al. 2022

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Current cranial repair techniques combine the use of autologous bone grafts and biomaterials. In addition to their association with harvesting morbidity, autografts are often limited by insufficient quantity of bone stock. Biomaterials lead to better outcomes, but their effectiveness is often compromised by the unpredictable lack of integration and structural failure. Bone tissue engineering offers the promising alternative of generating constructs composed of instructive biomaterials including cells or cell-secreted products, which could enhance the outcome of reconstructive treatments. This review focuses on cell-based approaches with potential to regenerate calvarial bone defects, including human studies and preclinical research. Further, we discuss strategies to deliver extracellular matrix, conditioned media and extracellular vesicles derived from cell cultures. Recent advances in 3D printing and bioprinting techniques that appear to be promising for cranial reconstruction are also discussed. Finally, we review cell-based gene therapy approaches, covering both unregulated and regulated gene switches that can create spatiotemporal patterns of transgenic therapeutic molecules. In summary, this review provides an overview of the current developments in cell-based strategies with potential to enhance the surgical armamentarium for regenerating cranial vault defects.

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Biomimetic versus sintered macroporous calcium phosphate scaffolds enhanced bone regeneration and human mesenchymal stromal cell engraftment in calvarial defects

Cited by 22 publications

References 76 publications

Multi-leveled Nanosilicate Implants Can Facilitate Near-Perfect Bone Healing

Multi-leveled Nanosilicate Implants Can Facilitate Near-Perfect Bone Healing

Amorphous calcium phosphate-coated surfaces as a model for bone microenvironment in prostate cancer

A Narrative Review of Cell-Based Approaches for Cranial Bone Regeneration

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