Biomineral interface with superior cell adhesive and antibacterial properties based on enzyme-triggered digestion of saliva acquired pellicle-inspired polypeptide coatings

Li, Jianshu; Ding, Chunmei; Wu, Mingzhen; Xu, Xinyuan; Ke, Xiang; Xu, Hao; Li, Juan; Lou, Feng; Zhou, Kai; Jiang, Haolun; Xu, Peng; Wang, Xianlong; Si, Longlong; Li, Jianshu

doi:10.1016/j.cej.2021.128955

Cited by 16 publications

(7 citation statements)

References 43 publications

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“…By measuring the number of bacteria in the different groups through quantification of OD 600 , it was found that bacterial growth was markedly lower on the proposed surface than in a control. ( Figure 6 (Bii)) [ 140 ].…”

Section: Bioinspired Antibacterial Surfacesmentioning

confidence: 99%

Recent Progress on Bioinspired Antibacterial Surfaces for Biomedical Application

et al. 2022

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Surface bacterial fouling has become an urgent global challenge that calls for resilient solutions. Despite the effectiveness in combating bacterial invasion, antibiotics are susceptible to causing microbial antibiotic resistance that threatens human health and compromises the medication efficacy. In nature, many organisms have evolved a myriad of surfaces with specific physicochemical properties to combat bacteria in diverse environments, providing important inspirations for implementing bioinspired approaches. This review highlights representative natural antibacterial surfaces and discusses their corresponding mechanisms, including repelling adherent bacteria through tailoring surface wettability and mechanically killing bacteria via engineering surface textures. Following this, we present the recent progress in bioinspired active and passive antibacterial strategies. Finally, the biomedical applications and the prospects of these antibacterial surfaces are discussed.

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Section: Bioinspired Antibacterial Surfacesmentioning

confidence: 99%

Recent Progress on Bioinspired Antibacterial Surfaces for Biomedical Application

et al. 2022

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“…Peptide DDDEEK is the analogue of the first six-amino acid sequence in statherin. It has the specific targeting ability for hydroxyapatite (HA) but excludes the hydrolysis sites in statherin, which enables the stable and strong anchoring on the tooth surface in the complex oral environment. , In light of this, this sequence can be utilized to construct an adaptive functional layer by integration with other moieties . It is well documented that PEG-modified peptides could enhance the hydrophilicity, physicochemical, and biological stability of peptides, which not only protect the antimicrobial moieties from being masked or broken down but also attenuate the damage to the surrounding tissue mucosa. − Moreover, targeting the acidic microenvironment has been reported to be efficient for dental caries prevention. − A few studies have explored the adaptive antibacterial ability of histidine-rich antimicrobial peptides (AMPs) against cariogenic bacteria such as Streptococcus mutans (S.…”

Section: Introductionmentioning

confidence: 99%

“…27,28 In light of this, this sequence can be utilized to construct an adaptive functional layer by integration with other moieties. 29 It is well documented that PEG-modified peptides could enhance the hydrophilicity, physicochemical, and biological stability of peptides, which not only protect the antimicrobial moieties from being masked or broken down but also attenuate the damage to the surrounding tissue mucosa. 30−32 Moreover, targeting the acidic microenvironment has been reported to be efficient for dental caries prevention.…”

Section: Introductionmentioning

confidence: 99%

Oral Environment-Adaptive Peptide–Polymer Conjugate for Caries Prevention with Targeting, Antibacterial, and Antifouling Abilities

Xin,

Zhang,

et al. 2024

Chem. Mater.

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The easy attachment of biofouling on the tooth surface benefits the development of dental plaque, which eventually leads to dental diseases, such as dental caries. It is difficult for traditional antimicrobial agents to achieve the desired effect due to the dilution of saliva and the decomposition of enzymes in the oral cavity. In addition, selective antimicrobial activity is highly required to maintain balanced healthy oral microflora. Here, inspired by the self-cleaning properties of oral mucosa, a peptide−polymer conjugate, DDDEEKRWRWRWC-PEG (DRWP), was developed that integrates targeting, antibacterial, and antifouling properties. The amino acids such as arginine and tryptophan on DRWP afford an environment-adaptive antibacterial ability against cariogenic bacteria upon (de)protonation. By virtue of the strong and stable affinity with the tooth surface, DRWP acts as a protective layer to inhibit the adhesion and colonization of nonspecific proteins and microbes, further preventing the formation of biofilms on the tooth surface. The experiment in vivo demonstrates that DRWP can effectively diminish the onset and severity of dental caries without impacting oral microbiota diversity and surrounding mucosal tissues. These findings prove the great promise of DRWP in mouthwash for caries prevention and other bacterial infectious dental diseases.

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“…Therefore, bactericides, such as antibacterial peptides, antibiotics, carbon nanotubes, quaternary ammonium salts, and metal nanoparticles, have been widely coated on the surfaces for bacteria killing. Among them, coatings bearing ammonium salts were determined to be successful materials for killing bacteria due to their ability to kill bacteria even at low concentrations. − The potential mechanism of these agents is that polymers bearing positive charge groups, such as poly(META), can interact with the negatively charged phospholipids on bacterial membranes. Then, the hydrophobic tail penetrates the membrane and disrupts it, resulting in cell death. − Farah et al prepared coatings with antimicrobial abilities based on quaternary ammonium polymers.…”

Section: Introductionmentioning

confidence: 99%

Mussel-Inspired Polymer-Based Coating Technology for Antifouling and Antibacterial Properties

Imbia,

Ounkaew,

Mao

et al. 2024

Langmuir

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Zwitterionic coatings provide a promising antifouling strategy against biofouling adhesion. Quaternary ammonium cationic polymers can effectively kill bacteria on the surface, owing to their positive charges. This strategy can avoid the release of toxic biocides, which is highly desirable for constructing coatings for biomedical devices. The present work aims to develop a facile method by covalently grafting zwitterionic and cationic copolymers containing aldehydes to the remaining amine groups of selfpolymerized dopamine. Reversible addition−fragmentation chain transfer polymerization was used to copolymerize either zwitterionic 2-methacryloyloxyethyl phosphorylcholine monomer (MPC) or cationic 2-(methacryloyloxy)ethyl trimethylammonium monomer (META) with 4-formyl phenyl methacrylate monomer (FPMA), and the formed copolymers poly(MPC-st-FPMA) and poly(META-st-FPMA) are denoted as MPF and MTF, respectively. MPF and MTF copolymers were then covalently grafted onto the amino groups of polydopamine-coated surfaces. PDA/MPF/MTF-coated surfaces exhibited antibacterial and antifouling properties against S. aureus, E. coli, and bovine serum albumin protein. In addition, they showed excellent viability of normal human lung fibroblast cells MRC-5. We expect the facile surface modification strategy discussed here to be applicable to medical device manufacturing.

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Biomineral interface with superior cell adhesive and antibacterial properties based on enzyme-triggered digestion of saliva acquired pellicle-inspired polypeptide coatings

Cited by 16 publications

References 43 publications

Recent Progress on Bioinspired Antibacterial Surfaces for Biomedical Application

Recent Progress on Bioinspired Antibacterial Surfaces for Biomedical Application

Oral Environment-Adaptive Peptide–Polymer Conjugate for Caries Prevention with Targeting, Antibacterial, and Antifouling Abilities

Mussel-Inspired Polymer-Based Coating Technology for Antifouling and Antibacterial Properties

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