Biomineralization inspired engineering of nanobiomaterials promoting bone repair

Oliveira, Francilio Carvalho; Carvalho, Jancineide Oliveira de; Magalhães, Leila S. S. M.; Silva, Juliana Marques da; Pereira, Saronny Rose; Júnior, Antônio Luíz Gomes; Soares, Liana Martha; Cariman, Laynna Ingrid Cruz; Silva, Ruan Inácio da; Viana, Bartolomeu C.; Filho, Edson Cavalcanti da Silva; Afewerki, Samson; Cunha, Helenilza Ferreira Albuquerque; Vega, Maria Letícia; Marciano, Fernanda Roberta; Lobo, Anderson Oliveira

doi:10.1016/j.msec.2020.111776

Cited by 19 publications

(16 citation statements)

References 44 publications

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“…The GNRs showed a toxicity and cytotoxicity at the concentrations of 60 and 120 µg/mL, and neither toxicity nor cytotoxicity was determined at the concentration of 30 µg/mL using the Allium cepa assay. The hemolysis test determined that the scaffolds with the concentration of 0.3 mg/cm 2 were not toxic, and corroborating data from the biochemical markers glutamic pyruvic transaminase, glutamic oxaloacetic transaminase, and urea showed no cytotoxicity, genotoxicity, or mutagenicity [128]. Ordered CNT-HA scaffolds with improved mechanical properties and accelerated cell growth in vitro or in vivo were prepared using agarose gel electrophoresis to imitate a pattern of CLG and HA hydrogel scaffolds (AG-CLG-o-CNT).…”

Section: Carbon Nanotubessupporting

confidence: 69%

Advances in Use of Nanomaterials for Musculoskeletal Regeneration

Jampílek

Plachá

2021

Pharmaceutics

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Since the worldwide incidence of bone disorders and cartilage damage has been increasing and traditional therapy has reached its limits, nanomaterials can provide a new strategy in the regeneration of bones and cartilage. The nanoscale modifies the properties of materials, and many of the recently prepared nanocomposites can be used in tissue engineering as scaffolds for the development of biomimetic materials involved in the repair and healing of damaged tissues and organs. In addition, some nanomaterials represent a noteworthy alternative for treatment and alleviating inflammation or infections caused by microbial pathogens. On the other hand, some nanomaterials induce inflammation processes, especially by the generation of reactive oxygen species. Therefore, it is necessary to know and understand their effects in living systems and use surface modifications to prevent these negative effects. This contribution is focused on nanostructured scaffolds, providing a closer structural support approximation to native tissue architecture for cells and regulating cell proliferation, differentiation, and migration, which results in cartilage and bone healing and regeneration.

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Section: Carbon Nanotubessupporting

confidence: 69%

Advances in Use of Nanomaterials for Musculoskeletal Regeneration

Jampílek

Plachá

2021

Pharmaceutics

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“…Analyzes of bone mineralization evolution were performed by histological analysis 45 days after implantation [ 21 , 22 ].…”

Section: Methodsmentioning

confidence: 99%

Nanocomposite Hydrogel Produced from PEGDA and Laponite for Bone Regeneration

Magalhães

Andrade

Bezerra

et al. 2022

JFB

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Herein, a nanocomposite hydrogel was produced using laponite and polyethylene-glycol diacrylate (PEGDA), with or without Irgacure (IG), for application in bone tissue regeneration. The nanocomposites were characterized by X-ray diffraction (XRD), Fourier-Transform infrared spectroscopy (FTIR), and thermal analysis (TG/DTG). The XRD results showed that the crystallographic structure of laponite was preserved in the nanocomposite hydrogels after the incorporation of PEGDA and IG. The FTIR results indicated that PEGDA polymer chains were entangled on laponite in hydrogels. The TG/DTG found that the presence of laponite (Lap) improved the thermal stability of nanocomposite hydrogel. The toxicity tests by Artemia salina indicated that the nanocomposite hydrogels were not toxic, because the amount of live nauplii was 80.0%. In addition, in vivo tests demonstrated that the hydrogels had the ability to regenerate bone in a bone defect model of the tibiae of osteopenic rats. For the nanocomposite hydrogel (PEGDA + Lap nanocomposites + UV light), the formation of intramembranous bone in the soft callus was more intense in 66.7% of the animals. Thus, the results presented in this study evidence that nanocomposite hydrogels obtained from laponite and PEGDA have the potential for use in bone regeneration.

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“…Priyadarsini et al [ 101 ] demonstrated teratogenic effects of GO nanosheets in Drosophila melanogaster ; however, apart from effects on the development, they carried out the comet assay in the hemocyte cells of the flies’ hemolymph and detected increased DNA damage. Oliveira et al [ 102 ] studied the biocompatibility and toxicity of a nanomaterial composed of graphene nanoribbons and nanohydroxyapatite, which are used as regenerative scaffolds in an in vivo osteopenia model. The standard comet assay was carried out in blood cells, bone marrow, and the liver of animals implanted with the graphene biomaterial and negative results were obtained in all conditions.…”

Section: Resultsmentioning

confidence: 99%

Genotoxicity of Graphene-Based Materials

et al. 2022

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Graphene-based materials (GBMs) are a broad family of novel carbon-based nanomaterials with many nanotechnology applications. The increasing market of GBMs raises concerns on their possible impact on human health. Here, we review the existing literature on the genotoxic potential of GBMs over the last ten years. A total of 50 articles including in vitro, in vivo, in silico, and human biomonitoring studies were selected. Graphene oxides were the most analyzed materials, followed by reduced graphene oxides. Most of the evaluations were performed in vitro using the comet assay (detecting DNA damage). The micronucleus assay (detecting chromosome damage) was the most used validated assay, whereas only two publications reported results on mammalian gene mutations. The same material was rarely assessed with more than one assay. Despite inhalation being the main exposure route in occupational settings, only one in vivo study used intratracheal instillation, and another one reported human biomonitoring data. Based on the studies, some GBMs have the potential to induce genetic damage, although the type of damage depends on the material. The broad variability of GBMs, cellular systems and methods used in the studies precludes the identification of physico-chemical properties that could drive the genotoxicity response to GBMs.

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Biomineralization inspired engineering of nanobiomaterials promoting bone repair

Cited by 19 publications

References 44 publications

Advances in Use of Nanomaterials for Musculoskeletal Regeneration

Advances in Use of Nanomaterials for Musculoskeletal Regeneration

Nanocomposite Hydrogel Produced from PEGDA and Laponite for Bone Regeneration

Genotoxicity of Graphene-Based Materials

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