Biomineralization-Inspired Synthesis of Cerium-Doped Carbonaceous Nanoparticles for Highly Hydroxyl Radical Scavenging Activity

Abstract

Cerium oxide nanoparticles recently have received extensive attention in biomedical applications due to their excellent anti-oxidation performance. In this study, a simple, mild, and green approach was developed to synthesize cerium-doped carbonaceous nanoparticles (Ce-doped CNPs) using bio-mineralization of bull serum albumin (BSA) as precursor. The resultant Ce-doped CNPs exhibited uniform and ultrasmall morphology with an average size of 14.7 nm. XPS and FTIR results revealed the presence of hydrophilic group on the surface of Ce-doped CNPs, which resulted in excellent dispersity in water. The CCK-8 assay demonstrated that Ce-doped CNPs possessed favorable biocompatibility and negligible cytotoxicity. Using H2O2-induced reactive oxygen species (ROS) as model, Ce-doped CNPs showed highly hydroxyl radical scavenging capability. Furthermore, flow cytometry and live-dead staining results indicated that Ce-doped CNPs protected cells from H2O2-induced damage in a dose-dependent effect, which provided a direct evidence for anti-oxidative performance. These findings suggest that Ce-doped CNPs as novel ROS scavengers may provide a potential therapeutic prospect in treating diseases associated with oxidative stress.

Preparation, characterization, and evaluation of antioxidant activity and bioavailability of a self-nanoemulsifying drug delivery system (SNEDDS) for buckwheat flavonoids

“…The hydroxyl radical scavenging activities of buckwheat flavonoid nanoemulsion and suspension and the ascorbic acid solution samples were measured as previously reported [ 19 , 20 ]. In brief, FeSO 4 solution (2.5 mM, 1 ml) was added to the samples and then H 2 O 2 (2.5 mM, 1 ml) and salicylic acid (2.5 mM, 1 ml) were added successively.…”

Nanomaterials Exhibiting Enzyme-Like Properties (Nanozymes): Current Advances and Future Perspectives

“…It has also been reported that CeNPs show an auto-regeneration process, whereby nanoparticles regenerate the Ce +3 oxidation state atoms from oxidized Ce +4 atoms (during the superoxide radical dismutation process), within a few days, which further makes CeNPs ready to neutralize another superoxide radical. Although some initial studies have reported that CeNPs could also scavenge hydroxyl radicals, however, any conclusive data was not shown as their EPR data suggest that CeNPs does not neutralize hydroxyl radicals (Heckert et al, 2008b; Zou et al, 2018). Our group has found that CeNPs have a high affinity for superoxide radicals and therefore eliminate them efficiently.…”

Oxoglaucine mediates Ca2+ influx and activates autophagy to alleviate osteoarthritis through the TRPV5/calmodulin/CAMK‐II pathway

“…Centrifuged agglomerated chondrocytes were fixed by 3% glutaraldehyde for 24 h. Osmium tetroxide was used to stabilize the phospholipid membranes of cells and organelles. The next item was dehydrated by a series of ethanol concentrations (50%, 70%, 80%, 90%, 100%) for 10‐min intervals, and then the samples were embedded in resin and sliced for toluidine blue staining (0.5%) (Zou et al, 2018). Images were captured by a Hitachi H‐7650 Transmission Electron Microscope (Hitachi, Japan).…”