Biomineralization of iron phosphate nanoparticles in yeast cells

He, Wen; Zhou, Weijia; Wang, Yingjun; Zhang, Xudong; Zhao, Hongxin; Li, Zhengmao; Yan, Shunpu

doi:10.1016/j.msec.2008.10.030

Cited by 43 publications

(19 citation statements)

References 18 publications

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“…It should be mentioned that the hydrophilic anion groups mainly serve for the following two purposes: (1) they provide oriented nucleation sites for target cations [28], and (2) they accumulate more negative charges on the biotemplate surface. More recently, our previous study confirmed that the yeast cell surface has surplus negative charges as a result of cultivation [29].…”

Section: Resultssupporting

confidence: 54%

High-performance TiO2 from Baker’s yeast

He¹,

Cui²,

Yue³

et al. 2011

Journal of Colloid and Interface Science

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Section: Resultssupporting

confidence: 54%

High-performance TiO2 from Baker’s yeast

He¹,

Cui²,

Yue³

et al. 2011

Journal of Colloid and Interface Science

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“…The IM protein Atm1p exports an unknown species thought to be involved in ISC biosynthesis. Deletion of these and other ISC-related proteins affords a common phenotype characterized by a respiration defect, the constitutive activation of iron regulon genes, the accumulation of Fe(III) phosphate nanoparticles in mitochondria and increased levels of ROS (11–13). …”

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confidence: 99%

Biophysical Investigation of the Iron in Aft1-1^up and Gal-YAH1 Saccharomyces cerevisiae

2011

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Aft1p is a major iron regulator in budding yeast Saccharomyces cerevisiae. It indirectly senses cytosolic Fe status and responds by activating or repressing iron regulon genes. Aft1p within the Aft1-1up strain has a single amino acid mutation which causes it to constitutively activate iron regulon genes regardless of cellular Fe status. This leads to elevated Fe uptake under both low and high Fe growth conditions. Ferredoxin Yah1p is involved in Fe/S cluster assembly, and Aft1p-targeted iron regulon genes are also up-regulated in Yah1p-depleted cells. In this study Mössbauer, EPR, and UV-vis spectroscopies were used to characterize the Fe distribution in Aft1-1up and Yah1p-depleted cells. Aft1-1up cells grown in low-Fe medium contained more Fe than do WT cells. A basal level of Fe in both WT and Aft1-1up cells was located in mitochondria, primarily in the form of Fe/S clusters and heme centers. The additional Fe in Aft1-1up cells was present as HS Fe(III) species. These species are in a non-mitochondrial location, assumed here to be vacuolar. Aft1-1up cells grown in high-Fe medium contained far more Fe than found in WT cells. The extra Fe was present as HS Fe(III) ions, probably stored in vacuoles, and as Fe(III) (phosphate) nanoparticles, located in mitochondria. Yah1p-deficent cells also accumulated nanoparticles in their mitochondria, but they did not contain HS Fe(III) species. Results are interpreted by a proposed model involving three homeostatic regulatory systems, including the Aft1 system, a vacuolar iron regulatory system and a mitochondrial Fe regulatory system.

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“…It should be mentioned that the hydrophilic anion groups mainly serve the following two purposes: (1) they provide oriented nucleation sites for target cations [33] and (2) they accumulate more negative charges on the biotemplate surface. More recently, our previous study confirmed that the yeast cell surface has surplus negative charges as a result of cultivation [34]. When cations were added into the bioemulsion, positively charged cations were combined with the negatively charged -COO -and -OPO 3 2-groups, and were selfassembled to the yeast cells' surface by electrostatic interaction and gene regulation in the yeast cells.…”

Section: Resultsmentioning

confidence: 75%

Large-scale synthesis of hierarchically mesoporous phosphate nanocomposites using yeast cells as the template reactor

He¹,

Zhang²,

Zhang³

et al. 2011

Res Chem Intermed

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Based on the principles of microbial fermentation, cytoarchitectonics, and biomineralization, we have developed a novel synthetic method by which the multilayered nanostructure of yeast cell tissues can be copied by phosphates. Taking living yeast cells as catalytic templates and reactors, a series of hierarchical mesoporous Ca-, Zn-, Mg-, and Ti-phosphate-protein nanocomposites with complex surface shapes are synthesized under ambient conditions, and their scale-up syntheses are carried out. The results show that the structure and composition of the synthesized nanocomposites have good repetition and they can be produced in a large quantity. The mechanism of biomineralization for gene regulation in yeast cells was discussed. The present method has many advantages, including the mild reaction conditions, simple process, no pollution, and ease of industrial production.

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Biomineralization of iron phosphate nanoparticles in yeast cells

Cited by 43 publications

References 18 publications

High-performance TiO2 from Baker’s yeast

High-performance TiO2 from Baker’s yeast

Biophysical Investigation of the Iron in Aft1-1^up and Gal-YAH1 Saccharomyces cerevisiae

Large-scale synthesis of hierarchically mesoporous phosphate nanocomposites using yeast cells as the template reactor

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Biomineralization of iron phosphate nanoparticles in yeast cells

Cited by 43 publications

References 18 publications

High-performance TiO2 from Baker’s yeast

High-performance TiO2 from Baker’s yeast

Biophysical Investigation of the Iron in Aft1-1up and Gal-YAH1 Saccharomyces cerevisiae

Large-scale synthesis of hierarchically mesoporous phosphate nanocomposites using yeast cells as the template reactor

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Product

Resources

About

Biophysical Investigation of the Iron in Aft1-1^up and Gal-YAH1 Saccharomyces cerevisiae