Biomineralization of N,N-dimethylformamide by Paracoccus sp. strain DMF

Swaroop, Shiv; Sughosh, P.; Ramanathan, G.

doi:10.1016/j.jhazmat.2009.05.138

Cited by 67 publications

(38 citation statements)

References 16 publications

(23 reference statements)

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“…There are three Paracoccus species known to be capable of DMF degradation: (i) P. aminophilus and P. aminovorans, isolated from soils taken from a factory which had produced DMF for about 20 years (43), and (ii) Paracoccus sp. strain DMF, isolated from activated sludge of domestic wastewater treatment unit in India (41).…”

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confidence: 99%

Plasmid pAMI2 of Paracoccus aminophilus JCM 7686 Carries N , N -Dimethylformamide Degradation-Related Genes Whose Expression Is Activated by a LuxR Family Regulator

Dziewit

Dmowski

Baj

et al. 2010

Appl Environ Microbiol

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N,N-Dimethylformamide (DMF), a toxic solvent used in the chemical industry, is frequently present in industrial wastes. Plasmid pAMI2 (18.6 kb) of Paracoccus aminophilus JCM 7686 carries genetic information which is crucial for methylotrophic growth of this bacterium, using DMF as the sole source of carbon and energy. Besides a conserved backbone related to pAgK84 of Agrobacterium radiobacter K84, pAMI2 carries a three-gene cluster coding for the protein DmfR, which has sequence similarities to members of the LuxR family of transcription regulators, and two subunits (DmfA1 and DmfA2) of N,N-dimethylformamidase, an enzyme of high substrate specificity that catalyzes the first step in the degradation of DMF. Genetic analysis revealed that these genes, which are all placed in the same orientation, constitute an inducible operon whose expression is activated in the presence of DMF by the positive transcription regulator DmfR. This operon was used to construct a strain able to degrade DMF at high concentrations that might be used in the biotreatment of DMF-containing industrial wastewaters. To our knowledge, this is the first study to provide insights into the genetic organization and regulation as well as the dissemination in bacteria of genes involved in the enzymatic breakdown of DMF.

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confidence: 99%

Plasmid pAMI2 of Paracoccus aminophilus JCM 7686 Carries N , N -Dimethylformamide Degradation-Related Genes Whose Expression Is Activated by a LuxR Family Regulator

Dziewit

Dmowski

Baj

et al. 2010

Appl Environ Microbiol

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“…These values range from 120 to 250 rpm [5,25]. In this work, two values of stirring speed were tested to check the effect of agitation on the degradation rate of thiocyanate.…”

Section: Selection Of Inoculum Size and Stirring Speedmentioning

confidence: 99%

Conditions and Mechanisms in Thiocyanate Biodegradation

Combarros¹,

Collado²,

Laca³

et al. 2015

JRST

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Presence of thiocyanate (SCN -) is a problem of considerable interest in many industrial wastewaters, where this contaminant often appears accompanied by secondary sources of carbon, nitrogen and/or sulphur. In order to understand the effect of these compounds on the biodegradability of SCN -, this work investigates how the bacterium Pararacoccus thiocyanatus utilises the three elements that form the molecule of thiocyanate and compare this behaviour with those obtained in presence of other sources of N, S and C. Result showed that the bacterium was capable of utilizing thiocyanate as the sole substrate, achieving specific biodegradation rates of approximate 1.20 mg SCN -(mg cell·h) -1 and eliminating initial thiocyanate concentrations up to 5,000 mg L -1 . Experimental data were successfully fitted to a Teisser model, assuming the existence of substrate inhibition obtaining values of μ max of 0.059 h -1 , K s of 790 mg L -1 and K i of 6,520 mg L -1 for free mineral medium. Presence of additional carbon and nitrogen sources implied catabolic repression of the biodegradation of thiocyanate. In this case, only concentrations lower than 3,500 mg L -1 could be treated, obtaining specific degradation rates of around 0.70 mg SCN -·(h·mg cell) -1 . Tessier model values showed a higher maximum specific growth rate (0.344 h -1 ), changing also the values for affinity and inhibition constants (1,150 mg L -1 and 1,730 mg L -1 , respectively).

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“…DMF is biodegradable only in small concentration. Biodegradation with special bacterial strains selected for this particular pollutant can be considered satisfactory [16][17][18]. Alternative methods could be the chemical oxidation, wet oxidation (WO), CWO or one of the Advanced Oxidation Processes (AOP's) [19], followed by biological treatment at a usual wastewater treatment plant (WWTP) [3,8,[20][21][22][23][24][25][26][27][28].…”

Section: Introductionmentioning

confidence: 99%