Biominerlization and Possible Endosulfan Degradation Pathway Adapted by Aspergillus niger

Abstract

Endosulfan is a chlorinated pesticide; its persistence in the environment and toxic effects on biota are demanding its removal. This study aims at improving the tolerance of the previously isolated fungus Aspergillus niger (A. niger) ARIFCC 1053 to endosulfan. Released chloride, dehalogenase activity, and released proteins were estimated along with analysis of endosulfan degradation and pathway identification. The culture could tolerate 1,000 mg/ml of technical grade endosulfan. Complete disappearance of endosulfan was seen after 168 h of incubation. The degradation study could easily be correlated with increase in released chlorides, dehalogenase activity and protein released. Comparative infrared spectral analysis suggested that the molecule of endosulfan was degraded efficiently by A. niger ARIFCC 1053. Obtained mass ion values by GC-MS suggested a hypothetical pathway during endosulfan degradation by A. niger ARIFCC 1053. All these results provide a basis for the development of bioremediation strategies to remediate the pollutant under study in the environment.

Occurrence of persistent organic pollutants and polycyclic aromatic hydrocarbons in sediments of the inner bay of Lake Titicaca, Puno-Perú

“…Studies show that the half-life of endosulfan sulfate is approximately 120 days, (Bhalerao, 2013), so the presence of this compound in the samples analyzed shows the constant use of this molecule in agricultural areas near the Inner Bay of Lake Titicaca.…”

“…All sediment samples analysis show the contamination by endosulfan sulfate in concentrations from 0.0004 to 0.006 μg/g, a stable metabolite of endosulfan (Snedeker & Hay, 2012;Bhalerao, 2013), nevertheless, its use was prohibited in Peru since 2012 by the national agricultural health service SENASA.…”

“…The microorganisms present in sediments are the main degraders of organic pollutants (Polder et al, 2016), but at the same time, these sediments have been receiving important loads of antibiotic residues, compounds that would be affecting the bacterial population in charge of the degradation of other types of organic pollutants (Bhalerao, 2013); causing slow degradation of the organic pollutants to be present in the sediments, impacting the ecosystem that inhabits it; on the other hand, this constant presence of anbiotic residues is facilitating the development of antibiotic resistance genes (ARG) (Mah & Toole, 2001;Cerqueira, Matamoros, Bayona, & Piña, 2019), a greater effect has been evidenced when there is a joint presence of heavy metals, nutrients and organic pollutants that makes the variation and distribution of ARG crucial (Chen et al, 2019), these conditions would be influencing the amount of POPs residues analyzed in sediment samples (Fernandes et al, 2020).…”

Review on Performance of Aspergillus and Penicillium Species in Biodegradation of Organochlorine and Organophosphorus Pesticides

“…A rapid complete mineralization of 1.0 g•L −1 endosulfan within a week by the A. niger ARIFCC 1053 strain was reported by Tejomyee [33]. It was suggested that the degradation was initiated by the oxidation to endosulfan sulfate and its hydrolysis to endosulfan diol.…”