Biomolecular changes and somatic mutations induced by UV laser irradiation at embryonic stage of<i>Bombyx mori</i>

Manjunatha, H.; Hosagoudar, S. R.

doi:10.1080/09553000600917241

Cited by 5 publications

(4 citation statements)

References 13 publications

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“…The strong reasons why we have preferred the egg/embryonic stage of B. mori in the present study are because (a) any modifications that induced at the cellular level of an embryo by UV laser irradiation become visible either at pupal or adult stages or even in both stages [4,5,] but such events has not been reported in B. mori due to HS; (b) the structural changes reported at the pupal and adult stages due to exposure of embryos to high doses of UV laser radiation are well correlated with the manifestation of some important proteins as a function [6,7] and (c) since proteomic changes if any take place in the B. mori embryo due to hot events of the environment remain enigmatic.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, protein and structural changes induced not only at the embryonic stage but also at larval stage due to UV laser treatment [6,7] substantiated the role of embryonic cells in determining and manifestation of the post-embryonic traits of B. mori. Moreover, exposure of rat embryos to the elevated temperature 43C for about 7 min induced craniofacial defects while nonteratogenic heat shock (42C for 10 min) cause embryos to acquire thermotolerance.…”

Section: * Corresponding Authormentioning

confidence: 99%

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Comparative proteome analysis and thermal stress induced changes in the embryo of poly- and bi-voltine strains of Bombyx mori

2017

J App Biol Biotech

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Proteins are the ultimate operating molecules producing the physiological effect in all the stages of an organism. As a consequence, proteome of the embryo play a pivotal role in determining the biological structures besides cellular organization and morphogenetic movements that occur during embryo development. But, changes in the environment temperature for few hours whether that alter the embryo development, protein expression and hatching of infants are unknown in Bombyx mori L. Hence, eggs of poly-and bi-voltine breeds were exposed to heat shock temperature of 35, 40 and 45°C for 2 h to measure tolerance level and differential expression of proteins in different age of the embryos. To facilitate comparative analysis among different breeds a comprehensive proteome map was constructed considering protein profile of different day's embryos. Interestingly, ~10 to 21 protein bands were recorded during embryo development -from ovipostion till hatchingalong with few new protein bands on day-6, which were differ in their intensity in different silkworm strains. Interestingly, no change in the protein pattern was observed in different days embryos exposed to thermal stress at 35 and 40°C. But degradation of 18 and 19 kDa proteins were recorded in all the days of embryos exposed to 45°C while four major protein bands remain unaltered. Further, the percent of hatching was slightly declined in 35 and 40°C and zero hatching was recorded in all the stages of eggs HS at 45°C. This clearly indicates that differential expression/degradation of proteins as a response to thermal stress not only influences the growth and development but also determine hatching of embryo. Accordingly, we suggest that embryonic protein map constructed in the present study shall be a valid reference for comparative analysis and hatching of embryos as one of the key traits to measure the rate of thermotolerance in the existing or new strains/breeds of B. mori.

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Section: Discussionmentioning

confidence: 99%

Section: * Corresponding Authormentioning

confidence: 99%

Comparative proteome analysis and thermal stress induced changes in the embryo of poly- and bi-voltine strains of Bombyx mori

2017

J App Biol Biotech

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“…To this end, Tazima (1941); Datta et al (1981); Sitarama Iyengar et al (1981); Subramanya and Sreerama Reddy (1982); Nangia (1986) and Yusifov et al (1991) induced useful mutations by ionizing and non-ionizing rays and some proved to be useful to the sericulture industry (Murakami & Imai 1974;Haung & Onimaru 1986;Lee et al 1989). Concomitantly, Manjunatha et al (2006) analyzed bimolecular changes induced by UV irradiations in egg and haemolymph of B. mori. The stimulatory effects of low-level energy, the alpha, beta, gamma rays (Abdel Salam & Mahmoud 1995;Petkov et al 1996), ultra violet rays (Chougale & More 1992), ultra sound (Surendra Nath et al 1995), heavy ions (Kotani et al 2002) and laser rays (Kanarev & Chan 1985) have enhanced silk production.…”

Section: Introductionmentioning

confidence: 99%

“…Application of He-Ne laser on different embryonic stages revealed the negative impact on biological and commercial traits of B. mori . Concomitantly, Manjunatha et al (2006) analyzed bimolecular changes induced by UV irradiations in egg and haemolymph of B. mori. Further, unlike other irradiation, somatic mutations induced by UV laser persisted through larval, pupal and adult stages and were categorically classified as continuous and discontinuous somatic mutations.…”

Section: Introductionmentioning

confidence: 99%

Impact of third and fourth harmonic laser irradiation at embryonic stage on biocommercial traits of Bombyx mori

Hosagoudar¹,

Hooli

Manjunatha

2010

Entomological Research

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In order to study the impact of third and fourth harmonic laser on bio‐commercial traits of silkworm Bombyx mori, silkworm eggs of 2, 8, 16 and 24 h old embryos were exposed for varying durations to both third and fourth harmonic lasers. Computation of data is explicit that the third harmonic laser had a positive influence for shorter duration exposures on all traits, like hatching percentage (15% increase), larval weight (14% increase), cocooning percentage (12% increase), cocoon weight (7% increase), shell weight (9% increase), shell ratio (15% increase) and fecundity (11% increase) with significant increases in the batches exposed for shorter durations (up to 90 sec) with respect to their controls. Thereafter levels decreased as the duration of exposure increased. Notably, fourth harmonic laser irradiation reveals the negative effect on all the traits studied in the present study. Surprisingly the filament length was decreased in all irradiated samples for both the third and fourth harmonic laser, expect for 24 h old embryos irradiated for 30 s by the third harmonic laser which showed a 6% increase over the control.

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Comprehensive analysis of differentially expressed proteins in the male and female Bombyx mori larval instars exposed to thermal stress

Punyavathi

Manjunatha

2020

Arch Insect Biochem Physiol

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Gender sensitivity to ambient heat, despite well known in insect species, how it manifests during young and late larval instars of Bombyx mori is still unclear. To uncover this cryptic feature, different instars male and female larvae were subjected to varied thermal stress separately and sex‐stage specific expression of proteins was investigated. Interestingly, heat shock proteins (HSPs) 90 and 70 were expressed differently in all the instars and also between male and female larvae as confirmed by immunoblot assay. Besides up‐ and downregulation of few HSPs and other normal proteins, discreet expression of protein was noticed in the two‐dimensional gel electrophoresis of male than female larvae which were identified as HSP70 by mass spectrometry. Furthermore, quantitative polymerase chain reaction results show 3.98‐ and 4.86‐fold higher levels of Bmhsp70 and Bmhsp90 transcripts in male and female larvae, respectively, as a response to 40°C heat shock (HS) treatment. Conversely, in spite of the massive production of HSPs due to HS at 45°C, all the larvae were found dead, which is a strong proof of concept for autophagy. Comparatively, female larvae HS at 40°C succeed to spin cocoons with increased weight and silk contents than non‐HS larvae. Comprehensively, in the present study, we have noticed a strong correlation for the first time that Bmhsp90 and Bmhsp70 genes expressions due to thermal stress are not only sex specific but also explicit preferential and coordinated action on survivability and biosynthetic potential of the silkworm, B. mori larvae during different instars.

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Biomolecular changes and somatic mutations induced by UV laser irradiation at embryonic stage ofBombyx mori

Cited by 5 publications

References 13 publications

Comparative proteome analysis and thermal stress induced changes in the embryo of poly- and bi-voltine strains of Bombyx mori

Comparative proteome analysis and thermal stress induced changes in the embryo of poly- and bi-voltine strains of Bombyx mori

Impact of third and fourth harmonic laser irradiation at embryonic stage on biocommercial traits of Bombyx mori

Comprehensive analysis of differentially expressed proteins in the male and female Bombyx mori larval instars exposed to thermal stress

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