Biomolecular self-assembly of micrometer sized silica beads on patterned glass substrates

Alberti, Martin; Yacoub-George, Erwin; Hell, Waltraud; Landesberger, Christof; Bock, Karlheinz

doi:10.1016/j.apsusc.2009.04.165

Cited by 5 publications

(6 citation statements)

References 21 publications

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“…To this end, we fabricated a novel type of particle‐decorated glass substrates, by using microstructured DNA patterns that were produced by microcontact printing. This patterning technique has a higher lateral resolution than the above described ink‐jet spotting (Figure ), which is limited to about 100 µm spot size and, thus, not suitable for single‐cells analyses . Microcontact printing is based on structured stamps made of the elastomer polydimethylsiloxan (PDMS) (Figures S13 and S14, Supporting Information).…”

Section: Cell Adhesion On Surface‐tethered Sinp Structuresmentioning

confidence: 99%

“…While all these methods work very well with the simple‐to‐handle AuNP, the adaptation of such strategies for assembly and patterning of SiNP is much less evolved because the synthesis of tailored DNA‐modified SiNP is not well developed . Proof‐of‐concept studies have demonstrated that micrometer‐sized silica beads are amenable to DNA‐based patterning of glass substrates and quantum‐dot encoded silica nanospheres can be applied for DNA hybridization assays . However, the systematic evaluation of the synthesis of optically traceable DNA‐SiNP and their utility in cell culture experiments has not yet been explored.…”

Section: Introductionmentioning

confidence: 99%

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Biopebbles: DNA‐Functionalized Core–Shell Silica Nanospheres for Cellular Uptake and Cell Guidance Studies

Leidner

Weigel

Bauer

et al. 2018

Adv Funct Materials

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The development of a versatile class of silica nanoparticles for cell studies is reported. The particles contain a fluorescent dye-encoded core and a single-stranded DNA oligonucleotide-displaying shell. They are accessible in arbitrary size and color through robust protocols for Stöber-based colloidal synthesis and sturdy chemical surface functionalization. Silica particles in the size range of 100 nm to 1.5 µm diameter containing fluorescein, Cy3 oder Cy5 dye-encoded cores are synthesized and functionalized with DNA oligonucleotides. These silica biopebbles are conveniently traceable by microscopy and have a high affinity to live cells, which makes them ideal for cell uptake studies, as demonstrated for MCF7 and A431 cancer cells. The biopebbles can be utilized as building blocks for the self-assembled formation of arbitrary surface patterns on glass substrates. With these architectures, the privileged internalization of the biopebbles can be exploited for improved adhesion and guidance of cells because the particles are no longer ingested by adhered cells due to their physical connection with the solid support. It is believed that the biopebble approach will be useful for a variety of applications, fundamental studies in cell biology and tissue engineering.

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Section: Cell Adhesion On Surface‐tethered Sinp Structuresmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Biopebbles: DNA‐Functionalized Core–Shell Silica Nanospheres for Cellular Uptake and Cell Guidance Studies

Leidner

Weigel

Bauer

et al. 2018

Adv Funct Materials

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“…DNA‐directed immobilization of proteins and colloidal compounds on solid substrates bearing single‐stranded capture oligonucleotides provides an efficient means for the fabrication of functionalized surfaces that can be used for applications in sensing and cell culturing . While this approach has been utilized for a variety of AuNP‐based sensor platforms, its exploitation for SiNP is far less developed,7b,12 mainly due to the fact that DNA‐modified SiNP are less accessible than DNA‐modified AuNP . In the case of MSN, DNA‐modification is much better developed due to the fact that capping of MSN with DNA motifs is being utilized to install sophisticated gatekeeper systems that enable stimuli responsiveness and triggered release of encapsulated cargo .…”

Section: Introductionmentioning

confidence: 99%

Biopebble Containers: DNA‐Directed Surface Assembly of Mesoporous Silica Nanoparticles for Cell Studies

Sun

Leidner

Weigel

et al. 2019

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The development of methods for colloidal self‐assembly on solid surfaces is important for many applications in biomedical sciences. Toward this goal, described is a versatile class of mesoporous silica nanoparticles (MSN) that contain on their surface various types of DNA molecules to enable their self‐assembly into micropatterned surface architectures useful for cell studies. Monodisperse dye‐doped MSN are synthesized by biphase stratification and functionalized with an aptamer oligonucleotide that serves as gatekeeper for the triggered release of encapsulated molecular cargo, such as fluorescent dye rhodamine B or the anticancer drug doxorubicin. One or two additional types of oligonucleotides are installed on the MSN surface to enable DNA‐directed immobilization on solid substrates bearing patterns of complementary capture oligonucleotides. It is demonstrated that this strategy can be used for efficient self‐assembly of microstructured surface architectures, which not only promote the adhesion and guidance of cells but also are capable of affecting the fate of adhered cells through triggered release of their cargo. It is believed that this approach is useful for diverse applications in tissue engineering and nanobio sciences.

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“…In this regard, a promising tool is the very specific mechanism of DNA molecular recognition, which was already used for aggregation 29 and surface-pattern-assisted selfassembly 30 of gold nanoparticles, and also of larger spherical objects like micrometer-sized silica beads. 31 For our approach of self-assembly of spatial objects, we first studied the DNA-functionalization on flat substrate surfaces (Si, SiO 2 ) with amino-terminated oligonucleotides on 3-aminopropyltrimethoxysilane (APTMS) SAMs by coupling with glutaric aldehyde. The successful reaction is shown by patterning of the APTMS layers before DNA immobilization by microcontact printing or photolithography and the hybridization with fluorescence labeled complementary oligonucleotides (cDNA) afterward.…”

Section: Introductionmentioning

confidence: 99%

Toward Three-Dimensional Microelectronic Systems: Directed Self-Assembly of Silicon Microcubes via DNA Surface Functionalization

et al. 2013

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The huge and intelligent processing power of three-dimensional (3D) biological "processors" like the human brain with clock speeds of only 0.1 kHz is an extremely fascinating property, which is based on a massively parallel interconnect strategy. Artificial silicon microprocessors are 7 orders of magnitude faster. Nevertheless, they do not show any indication of intelligent processing power, mostly due to their very limited interconnectivity. Massively parallel interconnectivity can only be realized in three dimensions. Three-dimensional artificial processors would therefore be at the root of fabricating artificially intelligent systems. A first step in this direction would be the self-assembly of silicon based building blocks into 3D structures. We report on the self-assembly of such building blocks by molecular recognition, and on the electrical characterization of the formed assemblies. First, planar silicon substrates were functionalized with self-assembling monolayers of 3-aminopropyltrimethoxysilane for coupling of oligonucleotides (single stranded DNA) with glutaric aldehyde. The oligonucleotide immobilization was confirmed and quantified by hybridization with fluorescence-labeled complementary oligonucleotides. After the individual processing steps, the samples were analyzed by contact angle measurements, ellipsometry, atomic force microscopy, and fluorescence microscopy. Patterned DNA-functionalized layers were fabricated by microcontact printing (μCP) and photolithography. Silicon microcubes of 3 μm edge length as model objects for first 3D self-assembly experiments were fabricated out of silicon-on-insulator (SOI) wafers by a combination of reactive ion etching (RIE) and selective wet etching. The microcubes were then surface-functionalized using the same protocol as on planar substrates, and their self-assembly was demonstrated both on patterned silicon surfaces (88% correctly placed cubes), and to cube aggregates by complementary DNA functionalization and hybridization. The yield of formed aggregates was found to be about 44%, with a relative fraction of dimers of some 30%. Finally, the electrical properties of the formed dimers were characterized using probe tips inside a scanning electron microscope.

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Biomolecular self-assembly of micrometer sized silica beads on patterned glass substrates

Cited by 5 publications

References 21 publications

Biopebbles: DNA‐Functionalized Core–Shell Silica Nanospheres for Cellular Uptake and Cell Guidance Studies

Biopebbles: DNA‐Functionalized Core–Shell Silica Nanospheres for Cellular Uptake and Cell Guidance Studies

Biopebble Containers: DNA‐Directed Surface Assembly of Mesoporous Silica Nanoparticles for Cell Studies

Toward Three-Dimensional Microelectronic Systems: Directed Self-Assembly of Silicon Microcubes via DNA Surface Functionalization

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