Biomolecule Immobilization in Biosensor Development: Tailored Strategies Based on Affinity Interactions

Prieto‐Simón, Beatriz; Campàs, Mònica; Marty, J.-L.

doi:10.2174/092986608785203791

Cited by 69 publications

(49 citation statements)

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“…A variety of strategies have been reported for the immobilization of bioreceptors to sensor coatings [3,15,50,62,63,210]. Most of them can also be applied to the immobilization of b i o r e c e p t o r s t o a n t i f o u l i n g c o a t i n g s ( s e e a l s o "Immobilization of biorecognition elements" section).…”

Section: Functionalization Of Low-fouling Coatingsmentioning

confidence: 99%

Functionalizable low-fouling coatings for label-free biosensing in complex biological media: advances and applications

2015

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This review focuses on recent advances in the development of functionalizable antifouling coatings and their applications in label-free optical biosensors. Approaches to the development of antifouling coatings, ranging from self-assembled monolayers and PEG derivatives to ultra-low-fouling polymer brushes, are reviewed. Methods of preparation and characterization of antifouling coatings and the functionalization of antifouling coatings with bioreceptors are reviewed, and the effect of functionalization on the fouling properties of biofunctional coating is discussed. Special attention is given to biofunctional coatings for label-free bioanalysis of blood plasma and serum for medical diagnostics.

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Section: Functionalization Of Low-fouling Coatingsmentioning

confidence: 99%

Functionalizable low-fouling coatings for label-free biosensing in complex biological media: advances and applications

2015

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“…The biofunctionalization of chemical modified surfaces can be achieved in several manners that can be grouped in just two approaches: (i) direct adsorption and (ii) physical adsorption [29][30][31][32][33]: in both cases, each immobilization route presents advantages and drawbacks.…”

Section: Chemical Functionalization Proceduresmentioning

confidence: 99%

“…For proteins covalent coupling, amino, carboxylic, or thiol groups are preferred, whereas in the case of nucleic acids, it is possible to take advantage of the versatility of their synthesis to insert reactive groups at the end of the sequence. More difficult is the immobilization of immunoglobulins in a correct orientation, which can be achieved by controlled linkage of carbohydrates groups in the constant region or using affinity proteins (such as A or G Protein) [31]. In all physical adsorption types, a chemical modification of the platform surface is required to the extent that the material properties are tuned to accomplish the best analytical characteristics.…”

Section: Chemical Functionalization Proceduresmentioning

confidence: 99%

Bioengineered Surfaces for Real-Time Label-Free Detection of Cancer Cells

Martucci¹,

Migliaccio²,

ImmacolataRuggiero³

et al. 2016

Lab-on-a-Chip Fabrication and Application

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Biosensing technology is an advancing field that benefits from the properties of biological processes combined to functional materials. Recently, biosensors have emerged as essential tools in biomedical applications, offering advantages over conventional clinical techniques for diagnosis and therapy. Optical biosensors provide fast, selective, direct, and cost-effective analyses allowing label-free and real-time tests. They have also shown exceptional potential for integration in lab-on-a-chip (LOC) devices. The major challenge in the biosensor field is to achieve a fully operative LOC platform that can be used in any place at any time. The choice of an appropriate strategy to immobilize the biological element on the sensor surface becomes the key factor to obtain an applicable analytical tool. In this chapter, after a brief description of the main biofunctionalization procedures on silicon devices, two silicon-based chips that present an (i) IgG antibody or (ii) an Id-peptide as molecular probe, directed against the B-cell receptor of lymphoma cancer cells, will be presented. From a comparison in detecting cells, the Id-peptide device was able to detect lymphoma cells also at low cell concentrations (8.5 × 10 −3 cells/μm 2 ) and in the presence of a large amount of non-specific cells. This recognition strategy could represent a proof-of-concept for an innovative tool for the targeting of patient-specific neoplastic B cells during the minimal residual disease; in addition, it represents an encouraging starting point for the construction of a lab-on-a-chip system for the specific recognition of neoplastic cells in biological fluids enabling the follow-up of the changes of cancer cells number in patients, highly demanded for therapy monitoring applications.

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“…The immobilization of biorecognition elements plays a key role in biosensor construction [25]. Several methods, such as adsorption, entrapment, cross-linking and covalent binding, enable the immobilization of biomolecules on the solid surface of a transducer and are widely used in biosensor development.…”

Section: Introductionmentioning

confidence: 99%

Development of a Novel Optical Biosensor for Detection of Organophoshorus Pesticides Based on Methyl Parathion Hydrolase Immobilized by Metal-Chelate Affinity

Lan

Chen

Cui

et al. 2012

Sensors

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We have developed a novel optical biosensor device using recombinant methyl parathion hydrolase (MPH) enzyme immobilized on agarose by metal-chelate affinity to detect organophosphorus (OP) compounds with a nitrophenyl group. The biosensor principle is based on the optical measurement of the product of OP catalysis by MPH (p-nitrophenol). Briefly, MPH containing six sequential histidines (6× His tag) at its N-terminal was bound to nitrilotriacetic acid (NTA) agarose with Ni ions, resulting in the flexible immobilization of the bio-reaction platform. The optical biosensing system consisted of two light-emitting diodes (LEDs) and one photodiode. The LED that emitted light at the wavelength of the maximum absorption for p-nitrophenol served as the signal light, while the other LED that showed no absorbance served as the reference light. The optical sensing system detected absorbance that was linearly correlated to methyl parathion (MP) concentration and the detection limit was estimated to be 4 μM. Sensor hysteresis was investigated and the results showed that at lower concentration range of MP the difference got from the opposite process curves was very small. With its easy immobilization of enzymes and simple design in structure, the system has the potential for development into a practical portable detector for field applications.

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Biomolecule Immobilization in Biosensor Development: Tailored Strategies Based on Affinity Interactions

Cited by 69 publications

References 0 publications

Functionalizable low-fouling coatings for label-free biosensing in complex biological media: advances and applications

Functionalizable low-fouling coatings for label-free biosensing in complex biological media: advances and applications

Bioengineered Surfaces for Real-Time Label-Free Detection of Cancer Cells

Development of a Novel Optical Biosensor for Detection of Organophoshorus Pesticides Based on Methyl Parathion Hydrolase Immobilized by Metal-Chelate Affinity

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