Biomonitoring of DNA Damage in Photocopiers’ Workers From Peshawar, Khyber Pakhtunkhwa, Pakistan

Khisroon, Muhammad; Khan, Ajmal; Hassan, Nazia; Zaidi, Farrah; Farooqi, Javeed

doi:10.1097/jom.0000000000001964

Cited by 3 publications

(3 citation statements)

References 24 publications

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“…The Ag/Au BNPs showed an absorption peak at 542 nm, which is a good covenant with the previous results of the BNPs (Ag:Au) 1:1 ratio. [ 30 ] The temperature of the reaction medium determines the nature of NPs. [ 44 ] In this study, NPs were synthesized at varying temperature ranges (20°C to 100°C).…”

Section: Discussionmentioning

confidence: 99%

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Cytotoxicity and genotoxicity of green synthesized silver, gold, and silver/gold bimetallic NPs on BHK‐21 cells and human blood lymphocytes using MTT and comet assay

Sher

Ahmed

Mushtaq

et al. 2023

Applied Organom Chemis

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In cancer therapy, nanoparticles (NPs) shall be applied possibly by inoculation in the veins of humans. This action will connect them with white blood cells (WBCs) and red blood cells (RBCs) in the bloodstream before they reach their main targeted cancer cells. However, possible cytotoxicity and genotoxic effects of silver, gold, and silver/gold bimetallic NPs (Ag, Au, and Ag/Au BNPs) on baby hamster kidney-21 (BHK-21) cells and human blood lymphocytes were studied here by comet and MTT assays. Here, Ag, Au, and their Ag/Au BNPs were synthesized by using Hippeastrum hybridum (HH) extract. These NPs were studied by UV-visible spectroscopy, FT-IR, XRD, and EDX, and SEM analysis. XRD analysis conferring the crystal structure with an average size of 13.3, 10.72, and 8.34 nm of Ag, Au, and Ag/Au BNPs, respectively. SEM showed that Ag, Au, and Ag/Au BNPs had irregular morphologies, with nano measures calculated sizes of 40, 30, and 20 nm, respectively. EDX spectrometers confirmed the presence of elemental Ag signal of the AgNPs with 22.75%, Au signal of the AuNPs with 48.08%, Ag signal with 12%, and Au signal with 38.26% of the Ag/Au BNPs. The comet assay revealed that H 2 O 2 and BNPs prompt high rates of DNA damage compared to plant, Ag, Au NPs, and nontreated lymphocytes, which are represented as the total comet score (TCS). The BHK-21cells were incubated in the existence of doxorubicin, plant extract, Ag, Au, and Ag/Au BNPs. The cytotoxic effects could be observed in a dosedependent mode; doxorubicin and Ag/Au BNPs were more toxic than plant extract, Ag, and Au NPs. It is demonstrated that NPs interact with blood lymphocytes and BHK-21cells, causing significant DNA damage and reducing cell viability in a concentration-dependent manner. However, to reduce the potential threats of NPs, further studies are recommended.

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Section: Discussionmentioning

confidence: 99%

“…For the alkaline comet assay, [ 30 ] protocol was employed to test the genotoxicity of HH plant and their NPs. About 3 ml of blood samples were taken in K‐EDTA disposable tubes from a normal human.…”

Section: Methodsmentioning

confidence: 99%

Cytotoxicity and genotoxicity of green synthesized silver, gold, and silver/gold bimetallic NPs on BHK‐21 cells and human blood lymphocytes using MTT and comet assay

Sher

Ahmed

Mushtaq

et al. 2023

Applied Organom Chemis

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“…A study by Betha et al (2011) has identified photocopiers as the potential contributors of indoor air pollution and the presence of UFPs and VOCs in printer emissions, which together represent substantial health risks to the workers (20). Many researchers have reported on the effects of exposure to photocopier towards deoxyribonucleic acid (DNA) damage among operators in the printing centers (21)(22)(23). A study found that photocopier operators who were exposed to nanoparticles (NPs) experienced elevated levels of urinary biomarkers associated with oxidative damage, such as 8-hydroxydeoxyguanosine, 8-OHdG; 8-hydroxyguanosine, 8-OHG; and 8-isoprostane; 4-hydroxynonenal, HNE (23).…”

Section: Introductionmentioning

confidence: 99%

Association of Exposure to Indoor Air Pollutants and Respiratory Health Symptoms among Photocopy Workers in Bandar Baru Bangi, Selangor

Ghazali,

Jalaludin,

Junaidi

et al. 2023

MJMHS

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Introduction: Indoor air quality (IAQ) has a significant impact on individual well-being, particularly in photocopy industry workers who are exposed to high levels of contaminants. Improper IAQ is linked to various health issues including respiratory symptoms. This study aimed to determine the association between exposure to indoor air pollutants and respiratory health symptoms among photocopy workers in Bandar Baru Bangi, Selangor. Methods: A cross-sectional comparative study was conducted between exposed and control groups consisted of 76 employees who worked in photocopy premises and 76 employees who worked in offices. A set of questionnaires adapted from the American Thoracic Society was used to collect data on respiratory health symptoms, history of exposure, and socio-demographic background of the employees. Area monitoring was performed using various direct reading devices to measure UFP, PM2.5, PM10, CO, CO2, and TVOCs. Results: In all photocopy centers of exposed group, the mean of PM10 and PM2.5 were at 344.16 µg/m3 and 315.24 µg/m3 (p<0.001), which exceeded the acceptable limit of respirable particulates by ICOP at 150 µg/m3; UFP level at 9068.75 pt/cc (p<0.001); and TVOC at 2.32 ppm (p<0.001), below the limit of 3.0 ppm. High PM10 exposures was reported significantly associated with all respiratory symptoms at p<0.001 (cough OR=3.89 (95% CI=1.00–15.07); phlegm OR=4.82 (95% CI=1.19–14.60); wheezing OR=1.77 (95% CI=1.54–5.79); shortness of breath OR=2.13 (95% CI=0.82–5.55); chest tightness OR=0.37 (95% CI=0.14–0.98)); high PM2.5 exposure was significantly associated with all symptoms except for wheezing (cough at p<0.001, OR=6.71 (95% CI=1.41–32.00; phlegm at p<0.001, OR=5.15 (95% CI=1.41–32.00); shortness of breath at p<0.05, OR=2.13 (95% CI=0.85–5.32); and chest tightness at p<0.05, OR=0.37 (95% CI=0.15–0.95); meanwhile high exposure to UFP had significant relationships with shortness of breath at p<0.001, OR=0.66 (95% CI=0.27–1.64) and chest tightness at p<0.001, OR=1.51 (95% CI=0.61–3.74) among respondents. TVOC also had significant relationships with shortness of breath at p<0.001, OR=1.25, 95% CI=0.47–3.35 and chest tightness at p<0.001, OR=1.04, 95% CI=0.23–1.68. Results also showed that symptoms of cough, phlegm and wheezing were influenced by the PM10 and PM2.5 exposure at 49.4% (Nagelkerke R2= 0.494), 60.1% (Nagelkerke R2= 0.601) and 56.3% (Nagelkerke R2=0.563), respectively. However, there were no significant factors that influenced the symptom of shortness of breath and chest tightness among the respondents. Conclusion: This study concluded that exposure to indoor air pollutants increased the risk of respiratory health symptoms among photocopy workers. This study suggests that regular photocopy machine maintenance and daily workplace housekeeping is required, whereas the photocopy workers should be fully trained in terms of technical, health and safety aspects.

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Evaluation of Respiratory, Genotoxic and Cytotoxic Effects from Occupational Exposure to Typography Activities

et al. 2023

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This cross-sectional study was structured to allow the evaluation of the respiratory, genotoxic, and cytotoxic effects of occupational exposure to products resulting from the activity of printers in typographies and, to determine the risk of genotoxicity associated with such exposure. This study comprised 69 subjects, 25 individuals occupationally exposed to the products of typographies (study group), and 44 individuals non-exposed to the environment studied (reference group). The frequency of micronucleated cells and other nuclear anomalies (binucleated, karyolitic, pyknotic, and karyorrhectic cells) in the oral epithelia of each subject were analyzed. The frequency of micronucleated cells was significantly higher in the study group when compared to the reference one (12.96 MN/2000 cells vs. 4MN/2000 cells, respectively). Occupational exposure to products of typography is a risk factor for the occurrence of micronucleated cells in the study group (RR = 3.2; 95% CI, 2.7–3.9; p < 0.001). The results of the spirometry test did not reveal significant respiratory effects between the reference and study groups.

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Biomonitoring of DNA Damage in Photocopiers’ Workers From Peshawar, Khyber Pakhtunkhwa, Pakistan

Cited by 3 publications

References 24 publications

Cytotoxicity and genotoxicity of green synthesized silver, gold, and silver/gold bimetallic NPs on BHK‐21 cells and human blood lymphocytes using MTT and comet assay

Cytotoxicity and genotoxicity of green synthesized silver, gold, and silver/gold bimetallic NPs on BHK‐21 cells and human blood lymphocytes using MTT and comet assay

Association of Exposure to Indoor Air Pollutants and Respiratory Health Symptoms among Photocopy Workers in Bandar Baru Bangi, Selangor

Evaluation of Respiratory, Genotoxic and Cytotoxic Effects from Occupational Exposure to Typography Activities

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