Biomonitoring of methomyl pesticide by laccase inhibition on sensor containing platinum nanoparticles in ionic liquid phase supported in montmorillonite

Zapp, Eduardo; Brondani, Daniela; Vieira, Iolanda Cruz; Scheeren, Carla Weber; Dupont, Jaı̈rton; Barbosa, Antonio Marcos Jacques; Ferreira, Valdir Souza

doi:10.1016/j.snb.2011.04.015

Cited by 62 publications

(28 citation statements)

References 38 publications

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“…Moreover, the complete catalysis of the 4-AMP redox process was only observed in the pH range 4-6 when 60 min was applied as optimum incubation time. This optimum working range is in accordance to those found by other authors using electrochemical and spectroscopic techniques [20,46,47]. The optimum pH value was selected as 5.…”

Section: Optimization Of Experimental Electroanalytical Conditionssupporting

confidence: 81%

Biosensor based on multi-walled carbon nanotubes paste electrode modified with laccase for pirimicarb pesticide quantification

Oliveira

Barroso

Lima‐Neto

et al. 2013

Talanta

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This study focused on the development of a sensitive enzymatic biosensor for the determination of pirimicarb pesticide based on the immobilization of laccase on composite carbon paste electrodes. Multi-walled carbon nanotubes (MWCNTs) paste electrode modified by dispersion of laccase (3%, w/w) within the optimum composite matrix (60:40%, w/w, MWCNTs and paraffin binder) showed the best performance, with excellent electron transfer kinetic and catalytic effects related to the redox process of the substrate 4-aminophenol. No metal or anti-interference membrane was added. Based on the inhibition of laccase activity, pirimicarb can be determined in the range 9.90 × 10(-7) to 1.15 × 10(-5) mol L(-1) using 4-aminophenol as substrate at the optimum pH of 5.0, with acceptable repeatability and reproducibility (relative standard deviations lower than 5%). The limit of detection obtained was 1.8 × 10(-7) mol L(-1) (0.04 mg kg(-1) on a fresh weight vegetable basis). The high activity and catalytic properties of the laccase-based biosensor are retained during ca. one month. The optimized electroanalytical protocol coupled to the QuEChERS methodology were applied to tomato and lettuce samples spiked at three levels; recoveries ranging from 91.0 ± 0.1% to 101.0 ± 0.3% were attained. No significant effects in the pirimicarb electroanalysis were observed by the presence of pro-vitamin A, vitamins B1 and C, and glucose in the vegetable extracts. The proposed biosensor-based pesticide residue methodology fulfills all requisites to be used in implementation of food safety programs.

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Section: Optimization Of Experimental Electroanalytical Conditionssupporting

confidence: 81%

Biosensor based on multi-walled carbon nanotubes paste electrode modified with laccase for pirimicarb pesticide quantification

Oliveira

Barroso

Lima‐Neto

et al. 2013

Talanta

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“…The samples were mixed with ethanol and the extract was filtred before the analysis. Results agreed well with those measured by an HPLC official method, confirming the accuracy of the laccase biosensor (Zapp et al, 2011).…”

Section: Real Sample Applicationssupporting

confidence: 81%

Recent advances in biosensors based on enzyme inhibition

Amine

Arduini

Moscone

et al. 2016

Biosensors and Bioelectronics

190

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“…We observed a negative correlation between dissolved organic carbon (DOC) and laccase activity (Pearson, r = À.45, p < .05) as well as between Toxic Units (TU) and laccase activity (Pearson, r = À.50, p < .05). As suggested in others studies, we hypothesised that both DOC quantity and quality (Elisashvili, Penninckx, Kachlishvili, Asatiani, & Kvesitadze, 2006;Mikiashvili, Wasser, Nevo, & Elisashvili, 2006;Roman ı, Artigas, & Ylla, 2012) or the presence of some pesticide compounds could be important in laccase activity regulation (Oliveira et al, 2013;Zapp et al, 2011). Alternatively, shifts in fungal communities structure, highlighted by changes in fungal band richness, can partially explain the differences in laccase activity observed between sites and after translocation (Frey, Knorr, Parrent, & Simpson, 2004;Osono, 2007;Treton, Chauvet, & Charcosset, 2004).…”

Section: Discussionmentioning

confidence: 60%

“…As suggested in others studies, we hypothesised that both DOC quantity and quality (Elisashvili, Penninckx, Kachlishvili, Asatiani, & Kvesitadze, 2006;Mikiashvili, Wasser, Nevo, & Elisashvili, 2006;Roman ı, Artigas, & Ylla, 2012) or the presence of some pesticide compounds could be important in laccase activity regulation (Oliveira et al, 2013;Zapp et al, 2011). Here, differences were neither explained by nitrate concentrations nor by differences in fungal biomass (ergosterol concentration) since no differences were observed between sites.…”

Section: Discussionmentioning

confidence: 72%

Structural and functional responses of leaf‐associated fungal communities to chemical pollution in streams

2017

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Biomonitoring of methomyl pesticide by laccase inhibition on sensor containing platinum nanoparticles in ionic liquid phase supported in montmorillonite

Cited by 62 publications

References 38 publications

Biosensor based on multi-walled carbon nanotubes paste electrode modified with laccase for pirimicarb pesticide quantification

Biosensor based on multi-walled carbon nanotubes paste electrode modified with laccase for pirimicarb pesticide quantification

Recent advances in biosensors based on enzyme inhibition

Structural and functional responses of leaf‐associated fungal communities to chemical pollution in streams

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