Biomonitoring of mutagenicity and cytotoxicity in patients undergoing fixed orthodontic therapy

Angelieri, Fernanda; Carlin, Viviane; Martins, Renato A.; Ribeiro, Daniel Araki

doi:10.1016/j.ajodo.2009.06.029

Cited by 33 publications

(36 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…17,[19][20][21][22][23] The results presented by Angelieri et al and Heravi et al showed that the micronucleus frequencies were not significantly different before, during and after orthodontic treatment, and are in contrast with those of Natarajan et al, who found a significantly higher MN count in the test group at the day of debonding as compared to the control group without appliances. 20,22,23 Hafez et al 19 found that the cytotoxicity and genotoxicity of orthodontic appliances remained in the mouth for 6 months. According to Natarajan et al and Hafez et al fixed orthodontic appliances emit metal ions in sufficient quantities to induce a localized genotoxic effect, but these changes were not more evident after a longer time.…”

Section: Discussioncontrasting

confidence: 53%

“…22 There was no separate control group. The cells were collected by scraping the left and right cheek mucosa with a moist wooden spatula.…”

Section: Resultsmentioning

confidence: 99%

“…Additionally, in 3 of the studies, no control group was used. [21][22][23] Finally, ion release from fixed orthodontic devices in vivo is affected by various factors, such as saliva composition, pH, dietary habits, and microflora.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Trace metal ions release from fixed orthodontic appliances and DNA damage in oral mucosa cells by in vivo studies: A literature review

Downarowicz¹,

Mikulewicz²

2017

Adv Clin Exp Med

View full text Add to dashboard Cite

An overview of professional literature referring to the release of metal ions from fixed orthodontic appliances and their influence on oral mucosa in conditions of in vivo are presented, along with a detailed analysis of the exposure of the cells of cheek mucosa epithelium to metal ions. Electronic databases (PubMed, Elsevier, Ebsco) were searched with no language restrictions. The relevant orthodontic journals and reference lists were checked for all eligible studies. A total of 38 scientific articles were retrieved in the initial search. However, only 7 articles met the inclusion criteria. Statistically significant differences in the levels of the amount of nickel ions, cobalt ions and chromium ions were observed in cells of cheek mucosa. The most biocompatible material used in the production of fixed orthodontic appliances is titanium, and the least biocompatible material is steel, which releases the largest amount of nickel and chromium. Metal ions are released from fixed orthodontic appliances only in the first phase of treatment. It is recommended to conduct further, long-term research on a larger number of patients to define the influence of using fixed orthodontic appliances and biological effect they might have on tissues.

show abstract

Section: Discussioncontrasting

confidence: 53%

“…22 There was no separate control group. The cells were collected by scraping the left and right cheek mucosa with a moist wooden spatula.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Trace metal ions release from fixed orthodontic appliances and DNA damage in oral mucosa cells by in vivo studies: A literature review

Downarowicz¹,

Mikulewicz²

2017

Adv Clin Exp Med

View full text Add to dashboard Cite

show abstract

“…Studies in vitro have demonstrated that the RMGICs are capable of inducing cytotoxic effects on oral tissues, due to the presence of substances released during polymerization, such as hydrophilic monomers (Angelieri et al 2011, Corekci et al 2013, Selimović-Dragaš et al 2012, Xie et al 2008. Nevertheless, although these studies have evaluated the biologic properties of RMGICs, in vivo models are required to provide a more critical analysis with regard to the biocompatibility of these resin cements (Zhou et al 2011).…”

Section: Introductionmentioning

confidence: 99%

“…This material has been increasingly used by orthodontists for cementing orthodontic bands, due to the favorable physical and chemical properties capable of providing good retentiveness, fluoride release capacity and being practical to use (Malkoc et al 2010). However, there are indications that this cement may present cytotoxicity, genotoxicity and inadequate biocompatibility (Angelieri et al 2011, Selimović-Dragaš et al 2012.…”

Section: Introductionmentioning

confidence: 99%

Evaluation in vivo of biocompatibility of differents resin-modified cements for bonding orthodontic bands

Mesquita

Santos

Sampaio

et al. 2017

An. Acad. Bras. Ciênc.

View full text Add to dashboard Cite

The focus of this study was to test the hypothesis that there would be no difference between the biocompatibility of resin-modified glass ionomer cements. Sixty male Wistar rats were selected and divided into four groups: Control Group; Crosslink Group; RMO Group and Transbond Group. The materials were inserted into rat subcutaneous tissue. After time intervals of 7, 15 and 30 days morphological analyses were performed. The histological parameters assessed were: inflammatory infiltrate intensity; reaction of multinucleated giant cells; edema; necrosis; granulation reaction; young fibroblasts and collagenization. The results obtained were statistically analyzed by the Kruskal-Wallis and Dunn test (P<0.05). After 7 days, Groups RMO and Transbond showed intense inflammatory infiltrate (P=0.004), only Group RMO presented greater expression of multinucleated giant cell reaction (P=0.003) compared with the control group. After the time intervals of 15 and 30 days, there was evidence of light/moderate inflammatory infiltrate, lower level of multinucleated giant cell reaction and thicker areas of young fibroblasts in all the groups. The hypothesis was rejected. The Crosslink cement provided good tissue response, since it demonstrated a lower level of inflammatory infiltrate and higher degree of collagenization, while RMO demonstrated the lowest level of biocompatibility.

show abstract

Morphological and immunohistochemical analysis of the biocompatibility of resin‐modified cements

Mesquita

Santos

Godoy

et al. 2016

Microscopy Res & Technique

View full text Add to dashboard Cite

The aim of this double-blind randomized study was to evaluate the biocompatibility of resin-modified glass ionomer cements (RMGIC) by means of morphological and immunohistochemical analyses. RMGICs were selected and divided into four groups: Group CK (Crosslink Orthodontic Band Cement); Group RS (Resilience Light Cure Band Cement) Group RMO (RMO Band Cement), Group TP (Transbond Plus Light Cure Band), and Group C (Control-polyethylene). The materials were implanted in rat subcutaneous tissues, randomly selected for this study. After time intervals of 7, 15, and 30 days the tissues were submitted to morphological analysis. In immunohistochemical analysis, the immuno-marking of antibody CD68 was evaluated. The results obtained were statistically analyzed by the Kruskal-Wallis and Dunn tests (p < .05). In the morphological analysis after 7 days, Groups RS, RMO and TP showed more intense inflammatory infiltrate (p = .004) and only Group RMO presented greater intensity of multinucleated giant cells (p = .027). In the immunohistochemical analysis, Groups RMO and RS were observed to present a larger quantity of CD68+ (p = .004) in the time interval of 7 days and only Group RMO presented statistically significant difference for this parameter after 15 days (p = .026). In the time interval of 30 days, Group RMO presented the largest quantity of multinucleated giant cells (p < .004). The RMGICS Crosslink and Transbond Plus provided significantly better tissue biocompatibility than the Resilience and RMO Cements.

show abstract

Biomonitoring of mutagenicity and cytotoxicity in patients undergoing fixed orthodontic therapy

Cited by 33 publications

References 38 publications

Trace metal ions release from fixed orthodontic appliances and DNA damage in oral mucosa cells by in vivo studies: A literature review

Trace metal ions release from fixed orthodontic appliances and DNA damage in oral mucosa cells by in vivo studies: A literature review

Evaluation in vivo of biocompatibility of differents resin-modified cements for bonding orthodontic bands

Morphological and immunohistochemical analysis of the biocompatibility of resin‐modified cements

Contact Info

Product

Resources

About