Biomonitoring the genotoxic effects of pollutants on Tradescantia pallida (Rose) D.R. Hunt in Dourados, Brazil

Crispim, Bruno do Amaral; Vaini, Jussara Oliveira; Barufatti, Alexéia; Teixeira, Tatiane Zaratini; Mussury, Rosilda Mara; Seno, L. O.

doi:10.1007/s11356-011-0612-3

Cited by 32 publications

(27 citation statements)

References 16 publications

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“…Another plant used in the present study was T. pallida. However, few studies have used this species to evaluate the effects of pesticides (Raino et al, 2010) because it is most commonly used to evaluate environmental samples (Savóia et al, 2009;Crispim et al, 2012;Pereira et al, 2013). The results involving T. pallida indicate significant dose-dependent increases in the frequencies of MN in the groups exposed to the three IMI concentrations.…”

Section: Discussionmentioning

confidence: 99%

Allium cepa and Tradescantia pallida bioassays to evaluate effects of the insecticide imidacloprid

et al. 2015

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The indiscriminate use of pesticides has become a serious environmental concern. Of them, imidacloprid (IMI) is one of the most widely used worldwide. In 2010 in Brazil, 1.934 tonnes of IMI were sold and mainly used for sugarcane crops. Several studies have examined the toxicity of IMI as well as its possible ecological effects. However, few studies have examined its toxicity at the genetic level. This is one of the biggest challenges for the scientific community, which is concerned about the impacts of these contaminants on the environment and human health. In this study, we evaluated the effects of IMI above the genetic material in Allium cepa and Tradescantia pallida following exposure to different concentrations of this insecticide. The results demonstrated that the concentrations tested induced chromosomal alterations and increased the frequency of micronuclei. Therefore, IMI in these concentrations was genotoxic to the tested organisms. These factors should be taken into account when applying this pesticide.

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Section: Discussionmentioning

confidence: 99%

Allium cepa and Tradescantia pallida bioassays to evaluate effects of the insecticide imidacloprid

et al. 2015

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“…Despite technological developments in the production of less polluting fuels, motor vehicles are still considered an important mobile source of air pollution, difficult to monitor and control. In this scenario, biomonitoring can be used as a practical management tool in detecting and assessing air pollution, complementing physical and chemical methods (Crispim et al 2012). This alternative is especially useful when analyzing the accumulation of compounds with known carcinogenic potential, such as some polycyclic aromatic hydrocarbons and trace elements, which are not routinely measured by environmental agencies (Fajersztajn et al 2013).…”

Section: Introductionmentioning

confidence: 99%

“…purpurea Boom is an ornamental herbaceous, with a wide distribution in many countries. Several studies have used this species as an air quality biomonitor, especially for its genetic characteristics favorable for genotoxic analyses, more specific to mutation in the DNA of pollen mother cells, through micronucleus bioassay (micronucleus assay with Tradescantia pollen tetrads (Trad-MN)) (Batalha et al 1999;Carreras et al 2009;Mariani et al 2009;Alves et al 2011;Crispim et al 2012). However, only few of them have used T. pallida as a bioaccumulator of trace elements from urban air pollution (Saiki et al 2003;Sumita et al 2003).…”

Section: Introductionmentioning

confidence: 99%

Traffic-related air pollution biomonitoring with Tradescantia pallida (Rose) Hunt. cv. purpurea Boom in Brazil

Santos

Nadal

Schuhmacher

et al. 2015

Environ Monit Assess

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This study aimed to verify the capacity of Tradescantia pallida in the biomonitoring of air pollution in urban areas with different traffic intensities and under varying environmental conditions. Experiments were carried out in Ribeirão Preto, in the Southeastern Brazil, with more than 660,000 inhabitants and a fleet of more than 485,000 motor vehicles. Ten seedlings of T. pallida were exposed in three areas in the city, differing in traffic vehicle flow, in two seasons (wet and dry). At the end of each sampling period, which lasted 4 months, samples of leaves were collected, and the content of As, Ba, Ca, Cd, Cr, Cu, Fe, Hg, Mg, Mn, P, Pb, S, and Zn was determined by inductively coupled plasma mass spectroscopy (ICP-MS). The same elements were determined in soil samples for a seasonal characterization in conjunction with secondary data of environmental parameters. Additionally, micronucleus assay with early pollen tetrad cells of Tradescantia (Trad-MN) was conducted by collecting flower buds and analyzing the micronuclei frequencies in pollen mother cells. Although pollutant levels in air were below the Brazilian legal limits, plants exposed in the high-traffic flow area presented higher concentrations of elements related to vehicle emissions, especially under dry conditions, and higher micronuclei frequency in pollen mother cells. These results show the sensitivity of T. pallida to low-level urban air pollution and its suitability as bioindicator for trace elements. This alternative tool for biomonitoring can serve as a support methodology for the adoption of more restrictive public environmental policies in Brazil and extendible to other developing countries.

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“…Fonte: Guimarães et al, 2000;de Andrade Júnior et al, 2008;Mariani et al, 2009;Meireles et al, 2009;Savóia et al, 2009;Sisenando et al, 2011;Costa e Droste, 2012;Crispim et al, 2012;Teixeira e Barbério, 2012;Barbério et al, 2013;De Brito et al, 2013;Pereira et al, 2013;Blume et al, 2014;Crispim et al, 2014;Pereira et al, 2014;Yaguinuma et al, 2014;Santos et al, 2015;Sposito et al, 2015;Cassanego et al, 2015;da Costa et al, 2015;Caon et al, 2016;Campos et al, 2016;da Costa et al, 2016;Sposito et al, 2017. Juliana Caroline Vivian Sposito et al O processo de industrialização aumenta significativamente a contaminação atmosférica por misturas complexas de poluentes atmosféricos, como partículas, óxidos de nitrogênio e carbono (Costa e Droste, 2012).…”

Section: Resultsunclassified

Efetividade do ensaio Trad-MCN para avaliação de contaminantes atmosféricos em regiões brasileiras

Spósito¹,

Francisco²,

Barufatti³

2017

Rev. ambiente água

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RESUMOA planta Tradescantia pallida (Rose) D.R. Hunt var. purpurea tem sido utilizada em diversos modelos experimentais para detecção de danos morfofisiológicos e genéticos, por ser uma planta sensível a poluentes atmosféricos. No entanto, dentre os biotestes utilizados para avaliação do potencial mutagênico de contaminantes ambientais, o bioensaio de micronúcleo em Tradescantia (Trad-MCN) é um dos testes mais utilizados em estudos de monitoramento das condições atmosféricas. Diante do exposto, foi realizada uma revisão de literatura, identificando regiões brasileiras que utilizaram o bioensaio de micronúcleo em Tradescantia para monitorar alterações genéticas advindas da contaminação atmosférica. O levantamento de trabalhos indexados foi realizado em bancos de referência, como Portal de Períodicos CAPES, SciELO, ScienceDirect e PubMed, sendo os mesmos investigados quanto às regiões brasileiras biomonitoradas, período, área, exposição, monitoramento ativo ou passivo e planta natural ou clone. Os resultados indicaram que pesquisas baseadas no bioensaio Trad-MCN foram realizadas em diversas regiões brasileiras. Verificou-se que a maioria dos trabalhos são oriundos da região sudeste, especialmente do estado de São Paulo. Esse fato pode ser atribuído ao maior número de indústrias e automóveis em circulação existentes neste estado quando comparado aos demais, podendo interferir diretamente na qualidade do ar, despertando assim o interesse no meio científico. Com base nesta investigação, o bioensaio Trad-MCN revelou eficácia, e a planta Tradescantia demonstrou ser importante indicadora biológica de danos genéticos ocasionados pela exposição aos poluentes atmosféricos, e é amplamente utilizada na região sudeste do país.Palavras-chave: bioindicador, micronúcleos, Tradescantia. Effectiveness of the Trad-MCN assay for the evaluation of atmospheric contaminants in Brazilian regions ABSTRACTThe Tradescantia pallida (Rose) D.R. Hunt var. purpurea plant has been used in several experimental models to detect morphophysiological and genetic damage due its susceptibility to atmospheric pollutants. However, among the bioassays used to evaluate the mutagenic potential of environmental contaminants, the Tradescantia-Micronucleus (Trad-MCN)

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Biomonitoring the genotoxic effects of pollutants on Tradescantia pallida (Rose) D.R. Hunt in Dourados, Brazil

Abstract: Plants growing in localities with more intense vehicular traffic had greater quantities of micronuclei as well as higher frequencies and average numbers of stomata than localities with less traffic, indicating the presence of atmospheric contaminants that damaged their DNA.

Cited by 32 publications

References 16 publications

Allium cepa and Tradescantia pallida bioassays to evaluate effects of the insecticide imidacloprid

Allium cepa and Tradescantia pallida bioassays to evaluate effects of the insecticide imidacloprid

Traffic-related air pollution biomonitoring with Tradescantia pallida (Rose) Hunt. cv. purpurea Boom in Brazil

Efetividade do ensaio Trad-MCN para avaliação de contaminantes atmosféricos em regiões brasileiras

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