Biomphalaria glabrata immunity: Post-genome advances

Castillo, Maria G.; Humphries, Judith E.; Mourão, Marina Moraes; Marquez, Joshua; Gonzalez, Adrian; Montelongo, Cesar

doi:10.1016/j.dci.2019.103557

Cited by 43 publications

(41 citation statements)

References 246 publications

(353 reference statements)

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“…Sugar degradation is likely involved in energy generation for the bacteria. Polyamines are linked to bacterial growth and survival by reducing oxidative stress (Murray Stewart et al ., 2018) which could be generated by the presence of the hemocytes (Castillo et al ., 2020). Production of critical metabolites by the hemolymph microbiome could support sustainable production of these metabolites.…”

Section: Discussionmentioning

confidence: 99%

The hemolymph of Biomphalaria snail vectors of schistosomiasis supports a diverse microbiome

Chevalier

Diaz

McDew-White

et al. 2020

Environmental Microbiology

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The microbiome-the microorganism community that is found on or within an organism's body-is increasingly recognized to shape many aspects of its host biology and is a key determinant of health and disease. Microbiomes modulate the capacity of insect disease vectors (mosquitoes, tsetse flies, sandflies) to transmit parasites and disease. We investigate the diversity and abundance of microorganisms within the hemolymph (i.e. blood) of Biomphalaria snails, the intermediate host for Schistosoma mansoni, using Illumina MiSeq sequencing of the bacterial 16S V4 rDNA. We sampled hemolymph from five snails from six different laboratory populations of B. glabrata and one population of B. alexandrina. We observed 279.84 ± 0.79 amplicon sequence variants per snail. There were significant differences in microbiome composition at the level of individual snails, snail populations and species. Snail microbiomes were dominated by Proteobacteria and Bacteroidetes while water microbiomes from snail tank were dominated by Actinobacteria. We investigated the absolute bacterial load using qPCR: hemolymph samples contained 2784 ± 339 bacteria/μl. We speculate that the microbiome may represent a critical, but unexplored intermediary in the snail-schistosome interaction as hemolymph is in very close contact with the parasite at each step of its development.

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Section: Discussionmentioning

confidence: 99%

The hemolymph of Biomphalaria snail vectors of schistosomiasis supports a diverse microbiome

Chevalier

Diaz

McDew-White

et al. 2020

Environmental Microbiology

View full text Add to dashboard Cite

show abstract

“…Sugar degradation is likely involved in energy generation for the bacteria. Polyamines are linked to bacterial growth and survival by reducing oxidative stress [52] which could be generated by the presence of the hemocytes [53]. Production of critical metabolites by the hemolymph microbiome could support sustainable production of these metabolites.…”

Section: Discussionmentioning

confidence: 99%

“…We hypothesize that hemolymph microbiome could be a third player in the interaction between the snail and its schistosome parasite. The hemolymph is known to be involved in snail defense against schistosomes by the action of hemocytes and humoral factors [53]. Snail genetics has also been shown to be a major determinant of snail resistance [15].…”

Section: Discussionmentioning

confidence: 99%

The hemolymph ofBiomphalariasnail vectors of schistosomiasis supports a diverse microbiome

Chevalier

Diaz

McDew-White

et al. 2020

Preprint

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The microbiome – the microorganism community that is found on or within an organism’s body – is increasingly recognized to shape many aspects of its host biology and is a key determinant of health and disease. Microbiomes modulate the capacity of insect disease vectors (mosquitos, tsetse flies, sandflies) to transmit parasites and disease. We investigate the diversity and abundance of microorganisms within the hemolymph (i.e. blood) of Biomphalaria snails, the intermediate host for Schistosoma mansoni, using Illumina MiSeq sequencing of the bacterial 16S V4 rDNA. We sampled hemolymph from 5 snails from 6 different laboratory populations of B. glabrata and one population of B. alexandrina. We observed 281.34 ± 0.81 amplicon sequence variants (ASVs) per snail. There were significant differences in microbiome composition at the level of individual snails, snail populations and species. Snail microbiomes were dominated by Proteobacteria and Bacteroidetes while water microbiomes from snail tank were dominated by Actinobacteria. We investigated the absolute bacterial load using qPCR: hemolymph samples contained 2,620 ± 321 bacteria per μL. We speculate that the microbiome may represent a critical, but unexplored intermediary in the snail-schistosome interaction as hemolymph is in very close contact to the parasite at each step of its development.

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“…Some strains of B. glabrata, such as the NMRI and M line, are also susceptible to the infection by S. mansoni [22,23], while resistant strains such as BS-90 also allow infection with S. mansoni miracidia, though it does not lead to production of cercariae [24,25]. Snails have innate immune defense systems that can specifically or non-specifically target schistosomes for destruction [26,27]. Even following successful infection, miracidia and sporocysts are exposed to humoral factors, and attacked by motile hemocytes in the hemolymph and other tissues of the snail [28], resulting in production failure of cercariae.…”

Section: Discussionmentioning

confidence: 99%

Dynamics of Schistosoma Mansoni Development in The Intermediate Host, Biomphalaria Glabrata, A Freshwater Snail

Ouji¹,

Misu²,

Kitamura³

et al. 2020

Preprint

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Background Schistosomiasis is one of the most significant and prevalent waterborne parasitic diseases. Even though many studies have been reported about schistosomiasis, the dynamics of schistosome in intermediate host snails is little known. In the present study, the dynamics of Schistosoma larvae in infected snails was histologically investigated. Methods To examine the localization of Schistosoma mansoni (S. mansoni) parasites in the snails, Biomphalaria glabrata snails infected with miracidia were harvested and examined by stereoscopic observation. Then, frozen sections were prepared and stained with H&E. Furthermore, immunohistochemical detection of parasites was performed using anti-S. mansoni antibody, and their localization in the snails was analyzed. Results Snails infected with S. mansoni miracidia were harvested at 10 and 56 days post-infection (DPI) and analyzed. In the stereoscopic observations, white spots were observed at 56 DPI, while no spots were observed at 10 DPI. However, histological investigations visualized the larvae specifically in the head-foot area of the snail at 10 DPI. Further, it was observed that the larvae relocated to the hepatopancreas and ovotestis areas at 56 DPI. Conclusions The present study revealed the dynamics of Schistosoma larvae in intermediate snails, shown as the differential localization of S. mansoni larvae at early and late infection stages.

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Biomphalaria glabrata immunity: Post-genome advances

Cited by 43 publications

References 246 publications

The hemolymph of Biomphalaria snail vectors of schistosomiasis supports a diverse microbiome

The hemolymph of Biomphalaria snail vectors of schistosomiasis supports a diverse microbiome

The hemolymph ofBiomphalariasnail vectors of schistosomiasis supports a diverse microbiome

Dynamics of Schistosoma Mansoni Development in The Intermediate Host, Biomphalaria Glabrata, A Freshwater Snail

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