Bionanoconjugation via Click Chemistry:  The Creation of Functional Hybrids of Lipases and Gold Nanoparticles

Inorganic colloidal nanoparticles are very small, nanoscale objects with inorganic cores that are dispersed in a solvent. Depending on the material they consist of, nanoparticles can possess a number of different properties such as high electron density and strong optical absorption (e.g. metal particles, in particular Au), photoluminescence in the form of fluorescence (semiconductor quantum dots, e.g. CdSe or CdTe) or phosphorescence (doped oxide materials, e.g. Y 2 O 3 ), or magnetic moment (e.g. iron oxide or cobalt nanoparticles). Prerequisite for every possible application is the proper surface functionalization of such nanoparticles, which determines their interaction with the environment. These interactions ultimately affect the colloidal stability of the particles, and may yield to a controlled assembly or to the delivery of nanoparticles to a target, e.g. by appropriate functional molecules on the particle surface. This work aims to review different strategies of surface modification and functionalization of inorganic colloidal nanoparticles with a special focus on the material systems gold and semiconductor nanoparticles, such as CdSe/ZnS. However, the discussed strategies are often of general nature and apply in the same way to nanoparticles of other materials.

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“…By click-chemistry, lipase has been conjugated to Au nanoparticles (Brennan 2006), as well as peptides to gold rods (Oyelere et al 2007).…”

Section: (Iii) Peptides Proteins Enzymes and Antibodiesmentioning

confidence: 99%

Surface modification, functionalization and bioconjugation of colloidal inorganic nanoparticles

Sperling

Parak

2010

Phil. Trans. R. Soc. A.

1,409

1,092

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“…Due to their unique physical properties (81), they have been used to label antibodies (82), target polynucleotides (83), in cancer cell diagnostics (84), in drug delivery systems (85), etc. Many of these methods, however, rely on the non-covalent attachments of biological molecules, which have important drawbacks (86). For instance, non-covalent interactions are much weaker than covalent bonds, meaning that it is much easier to break apart the nanoparticle-biological molecule conjugate.…”

Section: Gold and Magnetic Nanoparticlesmentioning

confidence: 99%

“…For long-term conjugation, covalent bonds must be used. One study performed by Brennan et al showed that bioconjuga-tions to gold nanoparticles via covalent bonds could occur easily and rapidly with click chemistry (86).…”

Section: Gold and Magnetic Nanoparticlesmentioning

confidence: 99%

Click Chemistry, A Powerful Tool for Pharmaceutical Sciences

2008

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Abstract. Click chemistry refers to a group of reactions that are fast, simple to use, easy to purify, versatile, regiospecific, and give high product yields. While there are a number of reactions that fulfill the criteria, the Huisgen 1,3-dipolar cycloaddition of azides and terminal alkynes has emerged as the frontrunner. It has found applications in a wide variety of research areas, including materials sciences, polymer chemistry, and pharmaceutical sciences. In this manuscript, important aspects of the Huisgen cycloaddition will be reviewed, along with some of its many pharmaceutical applications. Bioconjugation, nanoparticle surface modification, and pharmaceutical-related polymer chemistry will all be covered. Limitations of the reaction will also be discussed.

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“…In these studies, no change in GNP size was observed and hydrophobic, organometallic and hydrophilic moieties could be introduced onto the particles. Brennan et al [34] used a similar biochemical approach to produce biomoleculemodified gold particles. They first produced azide-modified gold particles and 4-pentynoic acid-modified lipase and reacted the two using a copper(II) catalyst with ascorbatereducing agent to generate the active Cu(I) species in situ.…”

Section: Bioorthogonal Labelling For Clem Imagingmentioning

confidence: 99%

The potential of bioorthogonal chemistry for correlative light and electron microscopy: a call to arms

et al. 2015

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With correlative light and electron microscopy (CLEM), the ultrastructural cellular location of a biomolecule of interest can be determined using a combination of light microscopy (LM) and electron microscopy (EM). In many cases, the application of CLEM requires the use of markers that need to be attached to a biomolecule of interest to allow its identification and localization. Here, we review the potential of bioorthogonal chemistry to introduce such markers for CLEM.

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Bionanoconjugation via Click Chemistry: The Creation of Functional Hybrids of Lipases and Gold Nanoparticles

Cited by 245 publications

References 37 publications

Surface modification, functionalization and bioconjugation of colloidal inorganic nanoparticles

Surface modification, functionalization and bioconjugation of colloidal inorganic nanoparticles

Click Chemistry, A Powerful Tool for Pharmaceutical Sciences

The potential of bioorthogonal chemistry for correlative light and electron microscopy: a call to arms

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