Bionic Tiger‐Bone Powder Improves Bone Microstructure and Bone Biomechanical Strength of Ovariectomized Rats

Abstract

Objective To study the curative effect of bionic tiger‐bone powder on osteoporosis in ovariectomized rats and investigate its mechanism. Methods Overall, a 120 female Wistar rats were randomly divided into Sham (sham‐operated group), ovariectomy (OVX, ovariectomized group), TB (bionic tiger‐bone powder treatment group after ovariectomy) and TB + VD groups (bionic tiger‐bone powder + vitamin D treatment group after ovariectomy). The osteoporotic rat model was established 3 months after ovariectomy, and rats were intragastrically administrated with the corresponding drugs. Serum and bone tissue samples were collected from 10 rats in each group at weeks 4, 12 and 24 after intragastric administration. The bone microstructure of L6 vertebrae was analyzed by MicroCT, the biomechanical strength of left femurs was measured by the three‐point bending test, and serum bone metabolism markers (P1NP and CTX) were detected by ELISA. Changes in bone collagen were analyzed by Masson's trichrome staining and hydroxyproline detection, and members of the BMP2/SMAD/RUNX2 and OPG/RANKL/RANK signal pathways were detected by immunoblotting. Results Compared with the OVX group, the serum level of P1NP in the TB and TB + VD groups was higher (P < 0.05), while the CTX level was lower (P < 0.05). Bone collagen fiber structures in the TB and TB + VD groups were repaired, and the collagen content was significantly higher than that in the OVX group (P < 0.05). In the TB group, BMP‐2, P‐SMAD1/5, RUNX2 and OPG levels were increased in bone tissue (P < 0.01), RANKL levels were decreased (P < 0.01), and the bone microstructure and biomechanical strength were improved. Conclusion Bionic tiger‐bone powder promotes osteogenesis by activating the BMP2/SMAD/RUNX2 signaling pathway, suppresses osteoclasts by downregulating the OPG/RANK/RANKL signaling pathway, increases bone collagen content, and improves bone microstructure and bone biomechanical strength.

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“…5 for the results. DISCUSSION: TCM formulas not only reduce bone loss by decreasing bone resorption and increasing bone formation through multicomponent and multi-targets 21 , but also regulate the body's function in overall and relieve the pain in back and lumbago 22 . The herbal medicine that possess activity of replenishing kidney are often shown to have estrogen-like 23 , antioxidant activity or regulating the function of hypothalamuspituitary axis to enhance the estrogen level in serum and the herbal medicine that reinforce spleen can intensify the effects of to nifying kidney and herbal medicine activating blood can help active chemical constituents to arrive at the skeletonsite and regulate bone metabolism 24 .…”

Assessment of rhBMP-2-loaded bovine hydroxyapatite granules in the guided bone regeneration of critical bone defect in rat mandible bone

“… 10 The evidence shows that the administration of BMP-2 favours angiogenesis and osteogenesis, contributing to bone regeneration. 11 BMP-2 is an extracellular signalling molecule, an in vivo autocrine/paracrine factor 12 that promotes osteoregeneration and induces the differentiation of osteoblasts and the formation of bone, cartilage, and connective tissue. These outcomes, in turn, lead to an increase in the osteoinductive capacity of BMP-2 at local level.…”

Jintiange Capsule Alleviates Rheumatoid Arthritis and Reverses Changes of Serum Metabolic Profile in Collagen-Induced Arthritic Rats

“…In the middle-dose JTG-360 mg/kg group, the dose was converted from the drug clinical dose for humans, specifically the daily dose for animals = the daily dose for humans × 6.3/60. 24 …”

“…In the middle-dose JTG-360 mg/kg group, the dose was converted from the drug clinical dose for humans, specifically the daily dose for animals = the daily dose for humans × 6.3/60. 24 The CIA model was established in Wistar rats according to the reference methods. 37,38 Briefly, bovine CII was dissolved in 0.1 M acetic acid and emulsified in an equal volume of IFA at 4°C to obtain a final CII concentration of 1 mg/mL.…”

“…19 That bionic TB powder is prepared from the skeletons of several farmed edible animals including pigs, goats and deer. 19 Ample experimental studies and large clinical trials have demonstrated the prominent efficacies of JTG on alleviating osteoporosis in patients, [19][20][21][22][23] improving the bone microstructure, strengthening the bone biomechanical property in ovariectomized rats, 24 and curing patients with aromatase inhibitor-associated musculoskeletal symptoms. 25 Hence JTG has taken place of TB to treat osteoporosis in clinical for years, 26,27 and been listed as an effective treatment in the guidelines for the treatment of osteoporotic fractures since 2017.…”