Biophysical and biochemical characterization of active secondary metabolites from Aspergillus allahabadii

Rajamanikyam, Maheshwari; Gade, Sriramya; Vadlapudi, Varahalarao; Parvathaneni, Sai Prathima; Koude, Dhevendar; Dommati, Anand Kumar; Tiwari, Ashok Kumar; Misra, Sunil; Prabhakar, S.; Amanchy, Ramars; Upadhyayula, Suryanarayana Murty

doi:10.1016/j.procbio.2017.02.010

Cited by 18 publications

(24 citation statements)

References 48 publications

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“…β ‐Sitosterol‐3‐ O ‐ β ‐D‐glucoside (I), p ‐methoxy benzoic acid (II), di‐(2‐Ethylhexyl) phthalate (DEHP) (III), dibutyl phthalate (IV) (Figure ; Supplementary Material, Figures S2–S12; Table S1–S7).…”

Section: Resultsmentioning

confidence: 99%

Cytotoxic Potential of Green Synthesized Silver Nanoparticles of Lampranthus coccineus Extracts, Metabolic Profiling and Molecular Docking Study

et al. 2020

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The green synthesis of silver nanoparticles (SNPs) using plant extracts has gained great attention due to its unique properties. So, in this study, the aqueous and n-hexane extracts of Lampranthus coccineus F. Aizoaceae were used for the synthesis of silver nanoparticles. The early signs of SNPs synthesis were detected by a color change from yellow to reddish-brown color. The formation of SNPs was further confirmed using a transmission electron microscope (TEM), UV-Visible spectroscopy, and zeta potential. The TEM analysis of SNPs showed spherical nanoparticles with mean size ranges between 8.55 nm to 16.44 nm for Lampranthus coccineus aqueous and hexane extracts. The UV-Visible spectrophotometric analysis showed an absorption peak at λ max of 417 nm, which confirms the formation of SNPs. Zeta potential results indicated that the particles are negatively charged and fairly stable due to the electrostatic repulsion. The cytotoxic activity of the green synthesized SNPs as well as the four isolated compounds (β-Sitosterol-3-O-β-D-glucoside, P-methoxy benzoic acid, dibutyl phthalate, and dioctyl phthalate) was evaluated using MTT assay against MCF-7, HepG-2, and HCT-116 cell lines. Metabolic profiling of the annotated twelve compounds from the crude extract of L. coccineus. These compounds, along with the four isolated compounds were subjected to molecular docking study.

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Section: Resultsmentioning

confidence: 99%

Cytotoxic Potential of Green Synthesized Silver Nanoparticles of Lampranthus coccineus Extracts, Metabolic Profiling and Molecular Docking Study

et al. 2020

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“…The purification of ASJ‐M (40 g) on silica‐gel column chromatography using the mobile phase, ethyl acetate in hexane comprising multiple levels of purification provided five compounds, 1 – 5 . Compound 1 is a pale yellow oil, and a comparison of its spectral data with literature values [11] revealed it to be bis(2‐ethylhexyl) phthalate ( 1 ). Compound 2 is a white powder with a melting point of 157 °C.…”

Section: Resultsmentioning

confidence: 93%

A Coumarin−Donepezil Hybrid as a Blood−Brain Barrier Permeable Dual Cholinesterase Inhibitor: Isolation, Synthetic Modifications, and Biological Evaluation of Natural Coumarins

et al. 2022

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Plants have immensely contributed to the drug discovery for neurodegenerative diseases. Herein, we undertook the phytochemical investigation of Nardostachys jatamansi (D.Don) DC. rhizomes followed by semisynthetic modifications to discover cholinesterase (ChE) and beta-site amyloid precursor protein cleaving enzyme 1 (BACE-1) inhibitors. The 8-acetyl-7-hydroxycoumarin isolated from the bioactive extract moderately inhibits acetylcholinesterase (AChE) and BACE-1 with IC 50 values of 22.1 and 17.7 μM, respectively. The semisynthetic trifluoromethyl substituted coumarin chalcone display a 5-fold improvement in BACE-1 inhibition (IC 50 3.3 μM). Another semisynthetic derivative, a coumarin-donepezil hybrid, exhibits dual inhibition of both ChEs with IC 50 values of 1.22 and 3.09 μM, respectively. Molecular modeling and enzyme kinetics revealed that the coumarin-donepezil hybrid is a non-competitive inhibitor of AChE. It crosses the blood-brain barrier and also inhibits Aβ self-aggregation. The results presented herein warrant a detailed investigation of the coumarin-donepezil hybrid in preclinical models of Alzheimer's disease.

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“…Terrei members have also been known for their potential in producing secondary metabolites, such as A. terreus that produced ''Terrein'' (Goutam et al 2017), antimicrobial methyl 3,4,5-trimethoxy-2-(2-(nicotinamido) benzamido) benzoate (Wang et al 2011), and another antimicrobial compound against several fish pathogens (Edwardsiella tarda, Aeromonas hydrophila, Vibrio ordalii, and V. anguillarum) (Barakat & Gohar 2012), A. alabamensis that produced two diketomorpholine derivatives and a highly conjugated ergostane-type steroid that exhibited inhibitions against human pathogens (E. coli and M. luteus) and aquatic bacteria (E. ictaluri and V. alginolyticus) (Yang et al 2018) , A. allahabadii that produced antibacterial allahabadolactone B (2) and ( 22E)-5α,8αepidioxyergosta-6,22-dien-3β-ol against B. cereus (Sadorn et al 2016), and pyrone derivatives: 3-hydroxy 2-methyl 4-pyrone and 5-hydroxy-2-(hydroxymethyl)-4H-pyrone that showed potent antimicrobial, antioxidant, antidiabetic, and mosquito larvicidal activities (Rajamanikyam et al 2017), A. carneus produced a potential antimicrobial compounds prenylated indole alkaloids, carneamides A-C (1-3), quinazolinone derivatives, carnequinazolines A-C (5-7), aryl C-glycosides, carnemycin A, B (8, 9), and a drimane sesquiterpenoid (Zhuravleva et al 2012), A. niveus that produced a high antitumor, but weak antibacterial Aspochalamins A-D (Gebhardt et al 2004), A. microcysticus that produced a well-known antimicrobial compound, Asposterol (Heberle et al 1974).…”

Section: Discussionmentioning

confidence: 99%

Antagonistic activity of three Aspergillus isolates against Fusarium wilt of banana

Hidayat

Dewi

Sukmawati

2019

JMSB

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Endophytic fungi have been known to play a role in protecting their host plants against diseases and extreme environments. The purpose of this study was to determine the antagonistic activity of three Aspergillus isolates against F. oxysporum f. sp. cubense (Foc), and to identify the Aspergillus identity using multilocus phylogeny analysis. Antagonistic assay by dual culture method showed that the Aspergillus sp. strain PD2, strain PD4, and strain PD5 inhibited the growth of Foc isolate by 37.31%, 26.52%, and 12.04%, respectively. Multilocus phylogeny based on ITS rDNA, beta-tubulin, and calmodulin genes sequences showed that the Aspergillus strain PD2 and strain PD4 belong to A. section Terrei, while the Aspergillus sp. strain PD5 was identified as A. sydowii of the A. sect. Versicolores.

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Biophysical and biochemical characterization of active secondary metabolites from Aspergillus allahabadii

Cited by 18 publications

References 48 publications

Cytotoxic Potential of Green Synthesized Silver Nanoparticles of Lampranthus coccineus Extracts, Metabolic Profiling and Molecular Docking Study

Cytotoxic Potential of Green Synthesized Silver Nanoparticles of Lampranthus coccineus Extracts, Metabolic Profiling and Molecular Docking Study

A Coumarin−Donepezil Hybrid as a Blood−Brain Barrier Permeable Dual Cholinesterase Inhibitor: Isolation, Synthetic Modifications, and Biological Evaluation of Natural Coumarins

Antagonistic activity of three Aspergillus isolates against Fusarium wilt of banana

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