Biophysical and biological investigation of DNA nano-complexes with a non-toxic, biodegradable amine-modified hyperbranched polyester

Reul, Regina; Nguyen, Juliane; Biela, Adam; Marxer, Elena Eva Julianne; Bakowsky, Udo; Klebe, G.; Kissel, Thomas

doi:10.1016/j.ijpharm.2012.06.065

Cited by 17 publications

(7 citation statements)

References 27 publications

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“…Therefore, it is probable that the changes observed here are because of other alterations in the DNA arrangement such as the number of base pairs per helix and base angle relative to the helix axis. 42,43 The changes shown here in the circular dichroism profile of pDNA upon complexation with the cationic CD1 are similar to those reported by other groups. 38,[43][44][45] Differences in circular dichroism profiles may also possibly be attributed to changes in the localisation of the nucleic acid within complexes.…”

Section: Resultssupporting

confidence: 89%

“…42,43 The changes shown here in the circular dichroism profile of pDNA upon complexation with the cationic CD1 are similar to those reported by other groups. 38,[43][44][45] Differences in circular dichroism profiles may also possibly be attributed to changes in the localisation of the nucleic acid within complexes. Further studies, such as surface-enhanced Raman spectroscopy, would be required to fully elucidate this and are beyond the scope of this paper.…”

Section: Resultssupporting

confidence: 89%

See 1 more Smart Citation

Biophysical and Structural Characterisation of Nucleic Acid Complexes with Modified Cyclodextrins Using Circular Dichroism

O’Mahony

Cronin

Mcmahon

et al. 2014

Journal of Pharmaceutical Sciences

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Section: Resultssupporting

confidence: 89%

Section: Resultssupporting

confidence: 89%

Biophysical and Structural Characterisation of Nucleic Acid Complexes with Modified Cyclodextrins Using Circular Dichroism

O’Mahony

Cronin

Mcmahon

et al. 2014

Journal of Pharmaceutical Sciences

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“…The films were subsequently treated with the activated NHS‐diazirine, allowing “capping” of the polyamine surface and creating a poly‐diazirine graft. Thorough capping and an inactivation of the polyamines were important for two reasons: 1) any free amines will base‐catalyse the destruction of the polyester matrix ; and 2) free amines will give an unwanted electrostatic bioadhesion and may cause toxic cell membrane destabilization …”

Section: Resultsmentioning

confidence: 99%

Novel On‐Demand Bioadhesion to Soft Tissue in Wet Environments

Mogal

Papper

Chaurasia

et al. 2013

Macromolecular Bioscience

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Current methods of tissue fixation rely on mechanical-related technologies developed from the clothing and carpentry industries. Herein, a novel bioadhesive method that allows tuneable adhesion and is also applicable to biodegradable polyester substrates is described. Diazirine is the key functional group that allows strong soft tissue crosslinking and on-demand adhesion based on a free radical mechanism. Plasma post-irradiation grafting makes it possible to graft diazirine onto PLGA substrates. When the diazirine-PLGA films, placed on wetted ex vivo swine aortas, are activated with low intensity UV light, lap shear strength of up to 450 ± 50 mN cm(-2) is observed, which is one order of magnitude higher than hydrogel bioadhesives placed on similar soft tissues. The diazirine-modified PLGA thin films could be added on top of previously developed technologies for minimally invasive surgeries. The present work is focused on the chemistry, grafting, and lap shear strength of the alkyl diazirine-modified PLGA bioadhesive films.

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“…Theranostic nanoparticles require long-term stability in the bloodstream and rapid uptake by tumor cells [111]. Other cationic polymers used for gene transfection include HBP-DEAPA 60, a biodegradable amine modified hyperbranched polyester whose transfection efficiency was studied in A549 cells [112].…”

Section: Making Spions Feasible For Gene Therapy In Addition To the mentioning

confidence: 99%

Development of Magnetic Nanoparticles for Cancer Gene Therapy: A Comprehensive Review

2013

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Since they were first proposed as nonviral transfection agents for their gene-carrying capacity, magnetic nanoparticles have been studied thoroughly, bothin vitroandin vivo. Great effort has been made to manufacture biocompatible magnetic nanoparticles for use in the theragnosis of cancer and other diseases. Here we survey recent advances in the study of magnetic nanoparticles, as well as the polymers and other coating layers currently available for gene therapy, their synthesis, and bioconjugation processes. In addition, we review several gene therapy models based on magnetic nanoparticles.

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Biophysical and biological investigation of DNA nano-complexes with a non-toxic, biodegradable amine-modified hyperbranched polyester

Cited by 17 publications

References 27 publications

Biophysical and Structural Characterisation of Nucleic Acid Complexes with Modified Cyclodextrins Using Circular Dichroism

Biophysical and Structural Characterisation of Nucleic Acid Complexes with Modified Cyclodextrins Using Circular Dichroism

Novel On‐Demand Bioadhesion to Soft Tissue in Wet Environments

Development of Magnetic Nanoparticles for Cancer Gene Therapy: A Comprehensive Review

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