Biophysical characterization of asolectin-squalene liposomes

Costa, Maria Assunta; Mangione, Maria Rosalia; Santonocito, Radha; Passantino, Rosa; Giacomazza, Daniela; Librizzi, Fabio; Morán, Óscar; Carrotta, Rita

doi:10.1016/j.colsurfb.2018.06.032

Cited by 11 publications

(12 citation statements)

References 36 publications

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“…Naturally, Sq is characterized as a hydrophobic lipid, and its biological properties in cellular function might have been underestimated because of the low solubility. Thus, to overcome this water-insoluble property, a recent study proposed a lysosome-embedded Sq structure and showed its preferential cytotoxic effect on LAN5 cancer cells in their preliminary study ( Costa et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

New Amphiphilic Squalene Derivative Improves Metabolism of Adipocytes Differentiated From Diabetic Adipose-Derived Stem Cells and Prevents Excessive Lipogenesis

Ganbold

Ferdousi

Arimura

et al. 2020

Front. Cell Dev. Biol.

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Squalene (Sq) is a natural compound, found in various plant oils, algae, and larger quantity in deep-sea shark liver. It is also known as an intermediate of cholesterol synthesis in plants and animals including humans. Although evidences demonstrated its antioxidant, anticancer, hypolipidemic, and hepatoprotective and cardioprotective effects, its biological effects in cellular function might have been underestimated because of the water-insoluble property. To overcome this hydrophobicity, we synthesized new amphiphilic Sq derivative (HH-Sq). On the other hand, adipose-derived stem cells (ASCs) are a valuable source in regenerative medicine for its ease of accessibility and multilineage differentiation potential. Nevertheless, impaired cellular functions of ASCs derived from diabetic donor have still been debated controversially. In this study, we explored the effect of the HH-Sq in comparison to Sq on the adipocyte differentiation of ASCs obtained from subjects with type 2 diabetes. Gene expression profile by microarray analysis at 14 days of adipogenic differentiation revealed that HH-Sq induced more genes involved in intracellular signaling processes, whereas Sq activated more transmembrane receptor pathway-related genes. In addition, more important number of down-regulated and up-regulated genes by Sq and HH-Sq were not overlapped, suggesting the compounds might not only have difference in their chemical property but also potentially exert different biological effects. Both Sq and HH-Sq improved metabolism of adipocytes by enhancing genes associated with energy homeostasis and insulin sensitivity, SIRT1 , PRKAA2 , and IRS1 . Interestingly, Sq increased significantly early adipogenic markers and lipogenic gene expression such as PPARG , SREBF1 , and CEBPA , but not HH-Sq. As a consequence, smaller and fewer lipid droplet formation was observed in HH-Sq-treated adipocytes. Based on our findings, we report that both Sq and HH-Sq improved adipocyte metabolism, but only HH-Sq prevented excessive lipogenesis without abrogating adipocyte differentiation. The beneficial effect of HH-Sq provides an importance of synthesized derivatives from a natural compound with therapeutic potentials in the application of cell therapies.

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Section: Introductionmentioning

confidence: 99%

New Amphiphilic Squalene Derivative Improves Metabolism of Adipocytes Differentiated From Diabetic Adipose-Derived Stem Cells and Prevents Excessive Lipogenesis

Ganbold

Ferdousi

Arimura

et al. 2020

Front. Cell Dev. Biol.

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“…In this work, MLVs were prepared by Thin Layer Evaporation (TLE) followed by freezing and thawing (FAT) cycles. Afterword, UVs were obtained by extrusion through polycarbonate filters, following a well-known multistep procedure [ 25 ]. Importantly, the method here proposed for the determination of concentration needs two different and sequential extrusion steps, as follows: (I) the MLV solution, containing the lipid mass M 0 , is loaded into the syringe and the setup is sealed to proceed to the first extrusion (including 31 steps), ending with the recovery of the extruded volume, V 1 ; (II) a volume of solvent is then added into the loading syringe, taking care to not lose the sample and after sealing the setup again, the second extrusion (a few steps are needed) is performed, which gives the extruded volume V 2 .…”

Section: Resultsmentioning

confidence: 99%

“…The LS-based method can be applied also when dealing with liposomes encapsulating both hydrophilic or lipophilic molecules, as well as amphiphilic ones, at least when the molecule concentration regime assures solubility in lipids or in the water environment [ 25 ]. In fact, for hydrophilic molecules, only the background may slightly change, as the molecules present as a cosolute inside and outside the vesicles; at difference, lipophilic molecules are trapped in the lipid bilayer and the method can be applied by considering also the contribution to the weight concentration coming from the loaded molecules.…”

Section: Resultsmentioning

confidence: 99%

“…Moreover, it does not imply the use of organic solvents or other chemicals, and it is sensitive to the whole mass, including all the lipid components present in the nanosystem, proteins or drugs, if present. Then, a mass control of the sample could be obtained even though the matrix components are not well specified, like in the case of asolectin, a mixture of lipids often used to prepare liposomes for many different uses, such as for encapsulating active biomolecules [ 25 ]. Furthermore, to be applied, the method simply requires a reliable measurement of the intensity of the light scattered by vesicle dispersions, which can be achieved, of course, by using light scattering devices, but also by using, in an unconventional way, a spectrofluorimeter.…”

Section: Introductionmentioning

confidence: 99%

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Light Scattering as an Easy Tool to Measure Vesicles Weight Concentration

2020

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Over the last few decades, liposomes have emerged as promising drug delivery systems and effective membrane models for studying biophysical and biological processes. For all applications, knowing their concentration after preparation is crucial. Thus, the development of methods for easily controlling vesicles concentration would be of great utility. A new assay is presented here, based on a suitable analysis of light scattering intensity from liposome dispersions. The method, tested for extrusion preparations, is precise, easy, fast, non-destructive and uses a tiny amount of sample. Furthermore, the scattering intensity can be measured indifferently at different angles, or even by using the elastic band obtained from a standard spectrofluorimeter. To validate the method, the measured concentrations of vesicles of different matrix compositions and sizes, measured by light scattering with different angles and instruments, were compared to the data obtained by the standard Stewart assay. Consistent results were obtained. The light scattering assay is based on the assessment of the mass fraction lost in the preparation, and can be applied for methods such as extrusion, homogenization, French press and other microfluidic procedures.

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“…It is also the raw material of perfume and medicine. Squalene, as a nutritional supplement, has the effect of improving liver function and tissue activity [29][30][31]. Some bioactive components were found in benzene extracts which can be used in spices and food additives.…”

Section: Comparison Of Volatile Components In Two Extractsmentioning

confidence: 99%

Bioenergy and bioactive components in leaves of Toona sinensis

2020

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Toona sinensis is an important tree for urban greening construction. Toona sinensis sprouts are widely used because of their high medicinal and edible value. However, the functions and effective components of mature Toona sinensis leaves have not been fully exploited and utilized. In order to explore the value of Toona sinensis leaves in bioactive and bioenergy utilization, Toona sinensis leaves were extracted with benzene and ethanol, and then the components in extracts were analyzed by FT-IR and GC-MS. The results showed that benzene and ethanol extracts from Toona sinensis leaves mainly contained phenols, hydrocarbons, esters, alcohols, acids and other bioactive components. These bioactive components can be used in the fields of bioenergy, biomedicine, chemical row materials, food additives and spices. Among them, the content of bioactive components from benzene extract was higher than that from ethanol extract. These findings indicate that Toona sinensis leaves contain a large number of bioactive components which can be used in bioenergy and other fields, and have broad prospects for development.

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Biophysical characterization of asolectin-squalene liposomes

Cited by 11 publications

References 36 publications

New Amphiphilic Squalene Derivative Improves Metabolism of Adipocytes Differentiated From Diabetic Adipose-Derived Stem Cells and Prevents Excessive Lipogenesis

New Amphiphilic Squalene Derivative Improves Metabolism of Adipocytes Differentiated From Diabetic Adipose-Derived Stem Cells and Prevents Excessive Lipogenesis

Light Scattering as an Easy Tool to Measure Vesicles Weight Concentration

Bioenergy and bioactive components in leaves of Toona sinensis

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